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Establishment and application of the indicator amino acid oxidation method as a new protein requirement research method

Research Project

Project/Area Number 23617016
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Integrated Nutrition Science
Research InstitutionKyoto Prefectural University

Principal Investigator

KIDO Yasuhiro  京都府立大学, 生命環境科学研究科(系), 教授 (50195319)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsたんぱく質 / 代謝要求量 / 指標アミノ酸酸化法 / タンパク質
Research Abstract

The purpose of this study was to validate the conformity of the indicator amino acid oxidation method (IAAO) with the nitrogen balance method, and gather data on the protein metabolism using the IAAO method.
We established the IAAO method as a new protein requirement research method, conducted protein quality evaluations, evaluated the nutritional state of the proteins employed, and estimated the amount of metabolic demand for protein under various conditions using rats. In addition, we calculated protein requirements in adult and elderly Japanese subjects using the IAAO method. Furthermore, we clarified the relationship between the protein requirement for adult Japanese females and the estrus cycle.
Theses results suggested that the IAAO method conformed with results obtained using the nitrogen balance method. We expect that our results will be useful in the establishment of dietary reference intakes for protein for the Japanese, and contribute widely to health policies for Japanese.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (25 results)

All 2014 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 1 results) Presentation (19 results) Book (1 results)

  • [Journal Article] An evaluation of protein intake for metabolic demands and the quality of dietary protein in rats using an indicator amino acid oxidation method2011

    • Author(s)
      Aki Ogawa, Yuka Naruse, Yasutaka Shigemura, Yukiko Kobayashi, Isao Suzuki, Sayori Wada, Kohsuke Hayamizu, Masashi Kuwahata, and Yasuhiro Kido
    • Journal Title

      J. Nutr. Sci. Vitaminol

      Volume: 57(6) Pages: 418-425

    • NAID

      130002103052

    • Related Report
      2013 Final Research Report
  • [Journal Article] ラットにおける指標アミノ酸酸化法によるタンパク質必要量算出とタンパク質の質の評価2011

    • Author(s)
      小川亜紀,廣瀬太洋,桑波田雅士,鈴木公,木戸康博
    • Journal Title

      外科と代謝・栄養

      Volume: 45(5) Pages: 175-180

    • Related Report
      2013 Final Research Report
  • [Journal Article] 指標アミノ酸酸化法によるタンパク質必要量2011

    • Author(s)
      木戸康博,小川亜紀,廣瀬太洋,小林ゆき子,桑波田雅士
    • Journal Title

      アミノ酸研究

      Volume: 5(1) Pages: 7-11

    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Journal Article] An evaluation of protein intake for metabolic demands and the quality of dietary protein in rats using an indicator amino acid oxidation method2011

    • Author(s)
      Aki Ogawa, Yuka Naruse, Yasutaka Shigemura, Yukiko Kobayashi, Isao Suzuki, Sayori Wada, Kohsuke Hayamizu, Masashi Kuwahata, Yasuhiro Kido
    • Journal Title

      Journal of Nutritional Science and Vitaminology

      Volume: 57 Pages: 418-425

    • NAID

      130002103052

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] ラットにおける指標アミノ酸酸化法によるタンパク質必要量算出とタンパク質の質の評価2011

    • Author(s)
      小川亜紀, 廣瀬太洋, 桑波田雅士, 鈴木公, 木戸康博
    • Journal Title

      外科と代謝・栄養

      Volume: 45(5) Pages: 175-179

    • Related Report
      2011 Research-status Report
  • [Presentation] Protein requirement of young Japanese men using the indicator amino acid oxidation technique2013

    • Author(s)
      Yasuhiro Kido, Tahiro Hirose, Chikage Goto, Naoko Araki, Mamiko Sumi, Aki Ogawa, Kohsuke Hayamizu, Yukiko Kobayashi, Masashi Kuwahata
    • Organizer
      8th Asia pacific conference on clinical nutrition
    • Place of Presentation
      (Symposium 16-2)
    • Related Report
      2013 Final Research Report
  • [Presentation] 指標アミノ酸酸化法によるタンパク質の質の簡易的評価法への応用2012

    • Author(s)
      小川亜紀、速水耕介、川端二功、和田小依里、小林ゆき子、鈴木公、桑波田雅士、木戸康博
    • Organizer
      第66回日本栄養・食糧学会大会
    • Place of Presentation
      仙台(2J-5a)
    • Related Report
      2013 Final Research Report
  • [Presentation] A simple evaluation method for the quality of dietary protein in rats using an indicator amino acid oxidation method2012

    • Author(s)
      Aki Ogawa, Haruka Murayama, Chikage Goto, Yukiko Kobayashi, Kohsuke Hayamizu, Sayori Wada, Masashi Kuwahata, Yasuhiro Kido
    • Organizer
      16th International Congress of Dietetics 2012 (ICD 2012)
    • Place of Presentation
      Sydney Australia(1176)
    • Related Report
      2013 Final Research Report
  • [Presentation] 指標アミノ酸酸化法によるタンパク質の質の簡易的評価法の検討2012

    • Author(s)
      小川亜紀、村山陽香、小林ゆき子、桑波田雅士、木戸康博
    • Organizer
      第59回日本栄養改善学会学術総会
    • Place of Presentation
      名古屋(03-224-122)
    • Related Report
      2013 Final Research Report
  • [Presentation] 指標アミノ酸酸化法によるタンパク質の簡易的評価法の検討2012

    • Author(s)
      小川亜紀、村山陽香、速水耕介、横井香里、辻智子、桑波田雅士、木戸康博
    • Organizer
      日本アミノ酸学会第6回学術大会
    • Place of Presentation
      千葉(P-2)
    • Related Report
      2013 Final Research Report
  • [Presentation] 指標アミノ酸酸化法によるタンパク質の質の簡易的評価法の検討2012

    • Author(s)
      小川亜紀、村山陽香、速水耕介、横井香里、辻智子、桑波田雅士、木戸康博
    • Organizer
      日本アミノ酸学会第6回学術大会
    • Place of Presentation
      千葉大学
    • Related Report
      2012 Research-status Report
  • [Presentation] ラットにおける指標アミノ酸酸化法による食事タンパク質の質の評価2011

    • Author(s)
      小川亜紀、廣瀬太洋、小林ゆき子、桑波田雅士、木戸康博
    • Organizer
      第65回日本栄養・食糧学会大会
    • Place of Presentation
      東京(3Q-15p)
    • Related Report
      2013 Final Research Report
  • [Presentation] 指標アミノ酸酸化法を用いた日本人成人男性のたんぱく質必要量の算出2011

    • Author(s)
      廣瀬太洋、荒木直子、小川亜紀、和田小依里、小林ゆき子、桑波田雅士、木戸康博
    • Organizer
      第65回日本栄養・食糧学会大会
    • Place of Presentation
      東京(3Q-16p)
    • Related Report
      2013 Final Research Report
  • [Presentation] 指標アミノ酸酸化法を用いた日本人成人男性のたんぱく質代謝の測定2011

    • Author(s)
      廣瀬太洋、荒木直子、小川亜紀、和田小依里、小林ゆき子、桑波田雅士、木戸康博
    • Organizer
      日本外科代謝栄養学会第48回学術集会
    • Place of Presentation
      名古屋(AAS-07)
    • Related Report
      2013 Final Research Report
  • [Presentation] An evaluation of the quality of dietary protein using an IAAO method2011

    • Author(s)
      Aki Ogawa, Tahiro Hirose, Yukiko Kobayashi, Kohsuke Hayamizu, Masashi Kuwahata, Yasuhiro Kido
    • Organizer
      11th Asian Congress of Nutrition 2011 (ACN 2011)
    • Place of Presentation
      Singapore(PD1-002)
    • Related Report
      2013 Final Research Report
  • [Presentation] Estimation of protein requirement for young Japanese men using the indicator amino acid oxidation method2011

    • Author(s)
      Tahiro Hirose, Naoko Araki, Aki Ogawa, Yukiko Kobayashi, Sayori Wada, Masahi Kuwahata, Yasuhiro Kido
    • Organizer
      11th Asian Congress of Nutrition 2011 (ACN 2011)
    • Place of Presentation
      Singapore(PD3-069)
    • Related Report
      2013 Final Research Report
  • [Presentation] 指標アミノ酸酸化法を用いた日本人成人男性のたんぱく質代謝要求量の算出2011

    • Author(s)
      廣瀬太洋、荒木直子、小川亜紀、和田小依里、小林ゆき子、桑波田雅士、速水耕介、木戸康博
    • Organizer
      日本アミノ酸学会第5回学術大会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Final Research Report
  • [Presentation] ラットにおける指標アミノ酸酸化法による食事タンパク質の質の評価2011

    • Author(s)
      小川亜紀、廣瀬太洋、小林ゆき子、桑波田雅士、木戸康博
    • Organizer
      第65回日本栄養・食糧学会大会
    • Place of Presentation
      お茶の水女子大学(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] An evaluation of the quality of dietary protein using an IAAO method2011

    • Author(s)
      Aki Ogawa, Tahiro Hirose, Yukiko Kobayashi, Kohsuke Hayamizu, Masashi Kuwahata, Yasuhiro Kido
    • Organizer
      11th Asian Congress of Nutrition 2011 (ACN 2011)
    • Place of Presentation
      シンガポール
    • Related Report
      2011 Research-status Report
  • [Presentation] タンパク質・アミノ酸の必要量に関する研究(学会賞受賞講演)2011

    • Author(s)
      木戸康博
    • Organizer
      第58回日本栄養改善学会学術総会(招待講演)
    • Place of Presentation
      広島国際会議場(広島)
    • Related Report
      2011 Research-status Report
  • [Presentation] Protein requirement of young Japanese men using the indicator amino acid oxidation technique

    • Author(s)
      Yasuhiro Kido, Tahiro Hirose, Chikage Goto, Naoko Araki, Mamiko Sumi, Aki Ogawa, Kohsuke Hayamizu, Yukiko Kobayashi, Masashi Kuwahata
    • Organizer
      8th Asia Pacific Conference on Clinical Nutrition
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 指標アミノ酸酸化法によるタンパク質の質の簡易的評価法への応用

    • Author(s)
      小川亜紀、速水耕介、川端二功、和田小依里、小林ゆき子、鈴木公、桑波田雅士、木戸康博
    • Organizer
      第66回日本栄養・食糧学会大会
    • Place of Presentation
      東北大学(仙台)
    • Related Report
      2012 Research-status Report 2011 Research-status Report
  • [Presentation] A simple evaluation method for the quality of dietary protein in rats using an indicator amino acid oxidation method

    • Author(s)
      Aki Ogawa, Haruka Murayama, Chikage Goto, Yukiko Kobayashi, Kohsuke Hayamizu, Sayori Wada, Masashi Kuwahata, Yasuhiro Kido
    • Organizer
      16th International Congress of Dietetics 2012 (ICD 2012)
    • Place of Presentation
      シドニー(オーストラリア)
    • Related Report
      2012 Research-status Report
  • [Presentation] 指標アミノ酸酸化法によるタンパク質の質の簡易的評価法の検討

    • Author(s)
      小川亜紀、村山陽香、小林ゆき子、桑波田雅士、木戸康博
    • Organizer
      第59回日本栄養改善学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Book] アミノ酸科学の最前線—基礎研究を活かした応用戦略—, 2章9節アミノ酸の必要量2014

    • Author(s)
      木戸康博, (監修(鳥居邦夫,門脇基二))
    • Total Pages
      281
    • Publisher
      シーエムシー出版
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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