Complehensive search of lifespan-regulating genes via metabolism

Research Project

Project/Area Number	23617033
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Integrated Nutrition Science
Research Institution	Jin-ai University (2014) Kobe Gakuin University (2011-2013)
Principal Investigator	AMAKO Katsumi 仁愛大学, 人間生活学部, 准教授 (90275280)
Project Period (FY)	2011-04-28 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000) Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywords	寿命 / 活性酸素 / 遺伝子 / アスコルビン酸 / ビタミンC / 酵母 / 老化 / 抗酸化物質 / 酸化ストレス / 代謝
Outline of Final Research Achievements	Disruption of cytosolic superoxide dismutase in budding yeast leads decrase in growth rate, oxidative stress resistance, and chloronological lifespan. L-Galactose and D-arabinose make budding yeast accumlate ascorbate and erythroascorbate, and they partically complemented the phenotypes in superoxide dismutase disruption mutant. In addition, the complementation by direct addition of ascorbate to yeast liquid culture was accomplished with different manner to L-galactose and D-arabinose.