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APE1/ref1 gene provides anti-oxidative fate to Sca-1 positive cardiac progenitor cells and promotes cardiac repair via macrophage transition in ischemic environment

Research Project

Project/Area Number 23618002
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Regenerative medicine
Research InstitutionAsahikawa Medical College

Principal Investigator

TAKEHARA Naofumi  旭川医科大学, 医学部, その他 (90374793)

Co-Investigator(Kenkyū-buntansha) KAWABE Jun-ichi  旭川医科大学, 医学部, 特任准教授 (10400087)
HASEBE Naoyuki  旭川医科大学, 医学部, 教授 (30192272)
Co-Investigator(Renkei-kenkyūsha) MATSUBARA Hiroaki   (10239072)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
KeywordsApe1 / 心筋再生 / 抗炎症 / 虚血耐性 / 細胞移植 / 抗酸化 / 炎症 / 血管新生 / 国際情報交換 / 再生医学
Research Abstract

The ROS production and apoptosis stimulated by H2O2 were significantly reduced in Ape1-CPCs compared with DsRed-CPCs. At 4 weeks, the absolute change in the LVEF was significantly greater in Ape1-CPCs injected mouse, but not DsRed-CPCs, than in the placebo group, that was associated with reduced infarcted size. Ape1-CPCs injected mouse were significantly enhanced the engraft cells compared with DsRed-CPCs injected mouse. In infarct myocardium of Ape1-CPCs injected mouse, M1 macrophages, but not M2 macrophages, were significantly reduced compared with that of DsRed-CPCs injected mouse. APE1/ref1 gene enhances the survival of engraft Sca1-CPC accompanied with the redox effect against oxidative stress in ischemic myocardium. Great survived Sca1-CPCs repaired the loss of LV function, which were associated with reduced infarcted myocardium and inflammation via macrophage transition. These results may provide an APE1/ref1 gene as a novel target to innovate the cardiac cell-therapy.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (2 results)

All 2014

All Presentation (2 results)

  • [Presentation] Apurinic/apyrimidinic endonuclease/redox factor-1 gene provides anti-oxidative state to sca-1 positive cardiac progenitor cells and promotes cardiac repair in ischemic environment2014

    • Author(s)
      Tatsuya Aonuma, Naofumi Takehara, Maki Kabara, Kouki Matsuki, Atsushi Yamauchi, Jun-ichi Kawabe and Naoyuki Hasebe
    • Organizer
      ESC congress 2014
    • Year and Date
      2014-08-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Antioxidant effects of Apurinic/apyrimidinic endonuclease/redox factor-1 in Sca-1 positive cardiac progenitor cells promote cardiac repair via macrophage transition in an ischemic environment2014

    • Author(s)
      Tatsuya Aonuma*, Naofumi Takehara†, Maki Kabara*, Kouki Matsuki*, Atsushi Yamauchi*, Jun-ichi Kawabe† and Naoyuki Hasebe*
    • Organizer
      ヨーロッパ心臓病学会
    • Place of Presentation
      バルセロナ
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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