• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effect of hypoxia on cerebral cortex differentiation of iPC cells

Research Project

Project/Area Number 23618004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Regenerative medicine
Research InstitutionUniversity of Tsukuba

Principal Investigator

TAKASAKI Mami  筑波大学, 医学医療系, 特任助教 (80392009)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords低酸素応答 / 神経 / 胚性幹細胞 / iPS細胞 / 分化 / 神経外胚葉 / 再生医学 / 発生
Research Abstract

Embryonic stem (ES) cells are useful for elucidating the molecular mechanisms of cell fate decision in the early development of mammals. It has been shown that aggregate culture of ES cells efficiently induces neuroectoderm differentiation. However, the molecular mechanism that leads to selective neural differentiation in aggregate culture is not fully understood.
In this study, we demonstrate that the oxygen-sensitive hypoxia-inducible transcription factor, Hif-1a, is an essential regulator for neural commitment of ES cells. However, we could not observe Hif-1a activation in aggregate culture of human iPS cells at the timing of neural commitment. Further analysis might be necessary to elucidate molecular mechanism of neuroectoderm differentiation for iPS cells.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (7 results)

All 2014 2012 2011 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results) Remarks (3 results)

  • [Journal Article] Dual Functions of Hypoxia-Inducible Factor 1 Alpha for the Commitment of Mouse Embryonic Stem Cells Toward a Neural Lineage2014

    • Author(s)
      Zhao Y, Matsuo-Takasaki M, Tsuboi I, Kimura K, Salazar GT, Yamashita T, Ohneda O.
    • Journal Title

      Stem Cells and Development

      Volume: 印刷中 Issue: 18 Pages: 2143-2155

    • DOI

      10.1089/scd.2013.0278

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] The role of hypoxia inducible factor-1a in neural commitment of mouse embryonic stem cells2012

    • Author(s)
      高崎(松尾)真美
    • Organizer
      第10回国際幹細胞学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2012-06-15
    • Related Report
      2013 Final Research Report
  • [Presentation] The role of hypoxia inducible factor-1a in neural commitment of mouse embryonic stem cells2012

    • Author(s)
      高崎 真美
    • Organizer
      10th Annual Meeting, International Society for Stem Cell Research
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2012 Research-status Report
  • [Presentation] Hypoxia inducible factor 1a is positive regulator of mouse embryonic stem cell differentiation into neural progenitors2011

    • Author(s)
      高崎(松尾)真美
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-13
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/stemcell/paper.html

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://wakate.biol.tsukuba.ac.jp/index.html

    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/younginit/takasaki/ESteam/

    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi