• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Transcriptome analysis of cardiac progenitors to identify small RNAs that induce transdifferentiation

Research Project

Project/Area Number 23618009
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Regenerative medicine
Research InstitutionThe University of Kitakyushu

Principal Investigator

HIDAKA KYOKO  北九州市立大学, 基盤教育センター, 教授 (00216681)

Co-Investigator(Renkei-kenkyūsha) NAKAZAWA Koji  北九州市立大学, 国際環境工学部, 教授 (00304733)
SAKURAI Kazuo  北九州市立大学, 国際環境工学部, 教授 (70343431)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordsmiRNA / 分化転換 / 心筋細胞 / 多能性幹細胞 / 細胞分化
Research Abstract

In mammalian heart, cardiac muscle cells (cardiomyocytes) are terminally differentiated and their regenerative capacity is limited. Recent studies have demonstrated that a combination of transcription factors could reprogram fibroblast directly to cardiomyocyte-like cells, albeit at a low efficiency. On the other hand, microRNAs (miRNAs), a family of small RNAs, have also been shown to convert cell fate by regulating gene expression. Here, we identified miRNAs that were expressed in cardiac progenitor cells, by transcriptome analysis of murine ES cell-derived cells. We also found that a combination of these miRNAs could induce myocyte-specific genes in cardiac fibroblasts. These findings may improve direct reprogramming methods towards regenerative cardiac therapy.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (13 results)

All 2014 2013 2012 2011

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (9 results)

  • [Journal Article] The role of the helper lipid dioleoylphosphatidylethanol- amine (DOPE) for DNA transfection cooperating with a cationic lipid bearing ethylenediamine2013

    • Author(s)
      Mochizuki S, Kanegae N, Nishina K, Kamikawa Y, Koiwai K, Masunaga H, Sakurai K
    • Journal Title

      Biochim Biophys Acta

      Volume: Vol.1828, No.2 Issue: 2 Pages: 412-418

    • DOI

      10.1016/j.bbamem.2012.10.017

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of mouse embryoid bodies cultured on microwell chips with different well sizes2013

    • Author(s)
      K. Nakazawa, Y. Yoshiura, H. Koga, Y. Sakai
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: Vol.116, No.5 Issue: 5 Pages: 628-633

    • DOI

      10.1016/j.jbiosc.2013.05.005

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electrophysiological Properties of Prion-Positive Cardiac Progenitors Derived From Murine Embryonic Stem Cells2012

    • Author(s)
      Fujii H, Ikeuchi Y, Kurata Y et al.
    • Journal Title

      Circulation Journal

      Volume: 76 Issue: 12 Pages: 2875-2883

    • DOI

      10.1253/circj.CJ-12-0126

    • NAID

      10031126061

    • ISSN
      1346-9843, 1347-4820
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Embryoid body culture of mouse embryonic stem cells using microwell and micropatterned chips2011

    • Author(s)
      Sakai Y, Yoshiura Y, Nakazawa K
    • Journal Title

      J Biosci Bioeng

      Volume: Vol.111, No.1 Issue: 1 Pages: 85-91

    • DOI

      10.1016/j.jbiosc.2010.08.014

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] マイクロRNAによる心臓繊維芽細胞の心筋様細胞への分化誘導2014

    • Author(s)
      日高京子、中津可道、荒井勇二、森崎隆幸、續輝久
    • Organizer
      第5回Molecular Cardiovascular Conference II
    • Place of Presentation
      神戸ベイシェラトン&タワーズ(神戸市)
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス成体の骨格筋線維組成構築におけるVgll2の機能2013

    • Author(s)
      本多賢彦、日高京子、須川涼、深田宗一朗、住江訓明、森崎隆幸
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス成体の骨格筋線維組成構築におけるVgll2の機能2013

    • Author(s)
      本多賢彦、日高京子、須川涼、深田宗一朗、住江訓明、森崎隆幸
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Presentation] Vgll2は成体骨格筋線維型を制御する2012

    • Author(s)
      本多賢彦、日高京子、須川涼、深田宗一朗、住江訓明、森崎隆幸
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡市)
    • Related Report
      2013 Final Research Report
  • [Presentation] 筋分化にかかわるRNA結合タンパク質Rbm24の機能解析2012

    • Author(s)
      日高京子、金東浩、白井学、森崎隆幸
    • Organizer
      第3回Molecular Cardiovascular Conference II
    • Place of Presentation
      キロロ・ホテルピアノ(余市郡)
    • Related Report
      2013 Final Research Report
  • [Presentation] 筋分化にかかわるRNA結合タンパク質Rbm24の機能解析2012

    • Author(s)
      日高京子、金 東浩、白井 学、森崎 隆幸
    • Organizer
      第3回 Molecular Cardiovascular Conference II
    • Place of Presentation
      キロロ・ホテルピアノ(北海道余市郡)
    • Related Report
      2012 Research-status Report
  • [Presentation] Vgll2は成体骨格筋線維型を制御する2012

    • Author(s)
      本多 賢彦、日高 京子、須川 涼、 深田 宗一朗、住江 訓明、森崎 隆幸
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Vgll2 regulates myosin expression and affects muscle performance2011

    • Author(s)
      本多賢彦、日高京子、須川涼、深田宗一朗、住江訓明、森崎隆幸
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2013 Final Research Report
  • [Presentation] Vgll2 regulates myosin expression and affects muscle performance2011

    • Author(s)
      本多賢彦、日高京子、須川涼、深田宗一郎、住江訓明、森崎隆彦
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi