| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
A tagging system was developed to sort induced pluripotent stem (iPS) cells according to the expression levels of each of the four reprogramming factors. Using this system, the effective ratio (Oct3/4-high, Sox2-low, Klf4-high, c-Myc-high) was. To investigate the molecular basis, microarray analysis was performed. Pathway analysis revealed that the G protein-coupled receptor (GPCR) pathway was up-regulated significantly under the high efficiency condition and treatment with the chemokine, C-C motif ligand 2, a member of the GPCR family, enhanced somatic cell reprogramming. Furthermore, the genetic modifier, Whsc1l1 (variant 1), also improved the efficiency of somatic cell reprogramming.
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