Regeneration of islets used tissue engineering

• Project/Area Number: 23618012
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Regenerative medicine
• Research Institution: Fukuoka University
• Principal Investigator: KODAMA Shohta 福岡大学, 医学部, 准教授 (90549338)
• Co-Investigator(Kenkyū-buntansha): NISHINAKAMURA Hitomi 福岡大学, 膵島研究所, ポストドクター (90597692)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 組織工学的膵島 / 膵島再生 / 組織工学 / 再生医学・再生医療 / 膵島移植 / 血管再生 / 生体材料 / 糖尿病 / 再生医学 / 移植・再生医療 / トランスレーショナルリサーチ / 膵島細胞 / 神経再生

Research Abstract

Tissue engineered(TE) islets were reconstructed from dissociated islet cells originated from transgenic mice that expressed green fluorescence protein in mouse insulin promoter area. Although the reconstructive ratio of islets number was improved from 30% to 68%, it was not exceeded as primary number of native islets from one mouse. However, it was surprised that the content of insulin protein per DNA ratio was increased two fold up in TE islets.
Continuously, diabetic mice were transplanted TE islets under kidney capsule. TE islets ameliorated blood glucose levels using half number of islet cells to compare with native islets.

Research Products

• [Journal Article] Treating salivary gland hypofunction at both initial and advanced stages of Sjögren's like disease : a comparative study of bone marrow- versus spleen-cell therapy with a one-year monitoring period (2014)
  • Author(s): Khalili S., Faustman DL, Liu Y., Sumita Y., Blank D., Peterson A., Kodama S., Tran SD.
  • Journal Title: Cytotherapy Volume: 16 Pages: 412-23
  • Peer Reviewed
• [Journal Article] Treating salivary gland hypofunction at both initial and advanced stages of Sjögren’s like disease: a comparative study of bone marrow- versus spleen-cell therapy with a one-year monitoring period. (2014)
  • Author(s): Khalili S., Faustman DL, Liu Y., Sumita Y., Blank D., Peterson A., *Kodama S., *Tran SD. (*Corresponding Author.)
  • Journal Title: Cytotherapy Volume: 16 Pages: 412-23
  • Peer Reviewed
• [Journal Article] Edaravone, a Free Radical Scavenger, Accelerates Wound Healing in Diabetic Mice (2014)
  • Author(s): Naito R., Nishinakamura H., Watanabe T., Nakayama J., *Kodama S. (*Corresponding Author)
  • Journal Title: Wounds Volume: In Press
  • Peer Reviewed
• [Journal Article] HMGB1-mediated early loss of transplanted islets is prevented by anti-IL-6R antibody in mice. (2014)
  • Author(s): Itoh T., Nitta T., Nishinakamura H., Kojima D., Mera T., Ono J., Kodama S., Yasunami Y.
  • Journal Title: Pancreas Volume: In Press
  • Peer Reviewed
• [Journal Article] Pretreatment of donor islets with the Na+/Ca2+ exchanger inhibitor improves the efficiency of islet transplantation (2013)
  • Author(s): Mera T., Itoh T., Kita S., Kodama S. Kojima D., Nishinakamura H., Okamaoto K., Ohkura M., Nakai J., Iyoda T., Iwamoto T., Matsuda T., Baba A., Omori K., MullenY., Ono L., Watari H., Taniguchi M., Yasunami T.
  • Journal Title: Am J Transplant Volume: 13(8) Pages: 2154-60
  • Peer Reviewed
• [Journal Article] Vascular endothelial growth factor-C derived from CD11b+ cells induces therapeutic improvements in a murine model of hind limb ischemia (2013)
  • Author(s): Kuwahara G., Nishinakamura H., Kojima D., Tashiro T., Kodama S.
  • Journal Title: J Vasc Surg Volume: 57(4) Pages: 1090-9
  • Peer Reviewed
• [Journal Article] Vascular endothelial growth factor-C derived from CD11b+ cells induces therapeutic improvements in a murine model of hind limb ischemia. (2013)
  • Author(s): Kuwahara G., Nishinakamura H., Kojima D., Tashiro T., *Kodama S. (*Corresponding Author)
  • Journal Title: J Vasc Surg Volume: 57 Pages: 1090-1099
  • Peer Reviewed
• [Journal Article] Pretreatment of donor islets with the Na+/Ca2+ exchanger inhibitor improves the efficiency of islet transplantation. (2013)
  • Author(s): Mera T., Itoh T., Kita S., Kodama S. Kojima D., Nishinakamura H., Okamaoto K., Ohkura M., Nakai J., Iyoda T., Iwamoto T., Matsuda T., Baba A., Omori K., MullenY., Ono L., Watari H., Taniguchi M., Yasunami T.
  • Journal Title: Am J Transplant Volume: 13 Pages: 2154-2160
  • Peer Reviewed
• [Journal Article] Mesenchymal Stromal Cells Improve Salivary Function and Reduce Lymphocytic Infiltrates in Mice with Sjögren's-Like Disease (2012)
  • Author(s): Khalili S., Liu Y., Kornete M, Roescher N., Kodama S., Peterson A., Piccirillo CA., Tran SD.
  • Journal Title: PLoS ONE Volume: 7(6)
  • Peer Reviewed
• [Journal Article] Prevention of High-Mobility Group Box 1-Mediated Early Loss of Transplanted Mouse Islets in the Liver by Antithrombin III (2012)
  • Author(s): Kojima D., Mera T., Nishinakamura H., Itoh T., Ogata T., Matsuoka N., Kodama S., Yasunami Y.
  • Journal Title: Transplantation Volume: 93(10) Pages: 983-8
  • Peer Reviewed
• [Journal Article] Oriented Collagen Scaffolds for Tissue Engineering (2012)
  • Author(s): Isobe Y., Kosaka T., Kuwahara G., Mikami H., Saku T., Kodama S.
  • Journal Title: Materials Volume: 5(3) Pages: 501-11
  • Peer Reviewed
• [Journal Article] Two-Layered Tissue-Engineered Urethra Using Oral Epithelial and Muscle-Derived Cells (2012)
  • Author(s): Mikami H., Kuwahara G., Nakamura N., Yamato M., Tanaka M., Kodama S.
  • Journal Title: J Urology Volume: 187(5) Pages: 1882-9
  • Peer Reviewed
• [Journal Article] Two-Layered Tissue-Engineered Urethra Using Oral Epithelial and Muscle-Derived Cells. (2012)
  • Author(s): Mikami H., Kuwahara G., Nakamura N., Yamato M., Tanaka M., *Kodama S. (*Corresponding Author)
  • Journal Title: J Urol Volume: 187 Pages: 1882-1889
  • Peer Reviewed
• [Journal Article] Oriented Collagen Scaffolds for Tissue Engineering. (2012)
  • Author(s): Isobe Y., Kosaka T., Kuwahara G., Mikami H., Saku T., *Kodama S. (*Corresponding Author)
  • Journal Title: Materials Volume: 5 Pages: 501-511
  • Peer Reviewed
• [Journal Article] Prevention of High-Mobility Group Box 1-Mediated Early Loss of Transplanted Mouse Islets in the Liver by Antithrombin III. (2012)
  • Author(s): Kojima D., Mera T., Nishinakamura H., Itoh T., Ogata T., Matsuoka N., Kodama S., Yasunami Y.
  • Journal Title: Transplantation Volume: 93 Pages: 983-988
  • Peer Reviewed
• [Journal Article] Mesenchymal Stromal Cells Improve Salivary Function and Reduce Lymphocytic Infiltrates in Mice with Sjogren's-Like Disease. (2012)
  • Author(s): Khalili S., Liu Y., Kornete M, Roescher N., Kodama S., Peterson A., Piccirillo CA., Tran SD.
  • Journal Title: PLoS ONE Volume: 7
  • Peer Reviewed
• [Journal Article] Vascular endothelial growth factor-C derived from CD11b+ cells induces therapeutic improvements in a murine model of hind limb ischemia. (2012)
  • Author(s): Kuwahara G., Nishinakamura H., Kojima D., Tashiro T., *Kodama S. (*Corresponding Author)
  • Journal Title: J Vasc Surg Volume: - Issue: 4 Pages: 1-10
  • DOI: 10.1016/j.jvs.2012.08.121
  • Peer Reviewed
• [Journal Article] Two-Layered Tissue-Engineered Urethra Using Oral Epithelial and Muscle Derived Cells. (2012)
  • Author(s): Mikami H., Kuwahara G., Nakamura N., Yamato M., Tanaka M., *Kodama(*Correspondence)
  • Journal Title: J Urol (Cover Issue) Volume: 187 Pages: 1882-1889
  • Peer Reviewed
• [Journal Article] 移植早期膵島障害機序の解明と新規制御法開発 (2011)
  • Author(s): 米良利之、小玉正太、安波洋一
  • Journal Title: 膵臓 Volume: 26 Pages: 183-9
  • NAID: 10029435721
• [Journal Article] IKKβ regulates essential functions of the vascular endothelium through kinase-dependent and -independent pathways (2011)
  • Author(s): Ashida N, Senbanerjee S, Kodama S, Foo SY, Coggins M, Spencer JA, Zamiri P, Shen D, Li L, Sciuto T, Dvorak A, Gerszten RE, Lin CP, Karin M, Rosenzweig A.
  • Journal Title: Nat Commun Volume: 2 Issue: 1 Pages: 318-318
  • DOI: 10.1038/ncomms1317
  • Peer Reviewed
• [Journal Article] 移植早期膵島障害機構の解明と新規制御法開発 (2011)
  • Author(s): 米良利之、小玉正太、安波洋一
  • Journal Title: 膵臓 Volume: 26 Pages: 183-189
  • Peer Reviewed
• [Presentation] 脂肪組織由来間葉系幹細胞による移植膵島生着率改善と膵島量増加効果 (2014)
  • Author(s): 田中智子、伊東威、松本征仁、小島大望、米良利之、西中村瞳、小玉正太、小野順子、柳瀬敏彦、安波洋一
  • Organizer: 第41回日本膵・膵島移植研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-03-08
• [Presentation] 糖尿病モデルマウスにおけるフリーラディカルスカベンジャー、エダラボンの創傷治癒促進効果 (2014)
  • Author(s): 内藤玲子、西中村瞳、渡辺俊明、中山樹一郎、小玉正太
  • Organizer: 第13回日本再生医療学会総会
  • Place of Presentation: 京都
  • Year and Date: 2014-03-05
• [Presentation] M-CSF・GM-CSF培養CD11b陽性細胞投与によるマウス虚血肢モデルを用いた血流改善効果の検討 (2014)
  • Author(s): 西中村瞳、桑原豪、小島大望、田代忠、小玉正太
  • Organizer: 第13回日本再生医療学会総会
  • Place of Presentation: 京都
  • Year and Date: 2014-03-05
• [Presentation] 単離ドナー膵島のNa+/Ca2+交換体を標的とした移植前治療による肝内移植早期膵島障害の制御 (2013)
  • Author(s): 伊東威、米良利之、喜多紗斗美、小島大望、西中村瞳、岩本隆宏、小玉正太、安波洋一
  • Organizer: 第49回日本移植学会総会
  • Place of Presentation: 京都
  • Year and Date: 2013-09-06
• [Presentation] 細胞移植治療の展望-膵島再生・血管再生プロジェクトを中心に- (2013)
  • Author(s): 小玉正太
  • Organizer: 第6回糖尿病・血管代謝再生研究会
  • Place of Presentation: 金沢
  • Year and Date: 2013-07-12
  • Invited
• [Presentation] 細胞移植治療 (2013)
  • Author(s): 小玉正太
  • Organizer: 第36回烏帽子会北九州支部記念学術講演
  • Place of Presentation: 小倉
  • Year and Date: 2013-06-29
  • Invited
• [Presentation] 細胞移植治療の展望 –膵島再生・血管再生プロジェクトを中心に- (2013)
  • Author(s): 小玉 正太
  • Organizer: 第6回糖尿病・血管代謝再生研究会
  • Place of Presentation: 金沢
  • Invited
• [Presentation] 単離ドナー膵島の Na/Ca 交換体を標的にした移植前治療による肝内移植早期膵等障害の新規制御法 (2013)
  • Author(s): 米良 利之、伊東 威、喜多 紗斗美、小島 大望、西中村 瞳、永石 晴美、小方 貴子、金城 亜哉、岩本 隆宏、小玉 正太、安波 洋一
  • Organizer: 第40回日本膵・膵島移植研究会
  • Place of Presentation: 高松、日本
• [Presentation] マウス虚血肢モデルを用いた、骨髄由来の培養CD11b 陽性細胞投与による血流改善効果の検討 (2013)
  • Author(s): 西中村 瞳、桑原 豪、田代 忠、小玉 正太*
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜、日本
• [Presentation] トロンボモデュリンによる移植早期膵島障害の抑制 (2012)
  • Author(s): 小島大望、米良利之、西中村瞳、伊東威、松岡信秀、小玉正太、安波洋一
  • Organizer: 第39回日本膵・膵島移植研究会
  • Place of Presentation: 旭川
  • Year and Date: 2012-03-10
• [Presentation] Vascular endothelial growth factor-C derived from CD11b positive macrophages induces therapeutic improvements in a murine model of hind limb ischemia. (2012)
  • Author(s): Kuwahara G., Nishinakamura H., Kojima D., Tashiro T., *Kodama S.
  • Organizer: The 2012 Vascular Annual Meeting presented by Society for Vascular Surgery
  • Place of Presentation: Washington DC, MD, U.S.A.
• [Presentation] CD11b 陽性細胞に由来する VEGF-C が誘導したマウス虚血肢改善効果 (2012)
  • Author(s): 桑原 豪、西中村 瞳、田代 忠、小玉 正太*
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜、日本
• [Presentation] トロンボモデュリンによる移植早期膵島障害の抑制 (2012)
  • Author(s): 小島大望、米良利之、西中村瞳、伊東威、松岡信秀、小玉正太、安波洋一
  • Organizer: 第39回日本膵・膵島移植研究会
  • Place of Presentation: 旭川、日本
• [Presentation] Two-layered tissue-engineered urethra using oral epithelial and muscle-derived cells (2011)
  • Author(s): Mikami H., Kuwahara G., Nakamura N., Yamato M.,Tanaka M., Kodama S.
  • Organizer: Tissue Engineering & Regenerative Medicine International Society(TERMIS)
  • Place of Presentation: Granada, Spain
  • Year and Date: 2011-06-07
• [Presentation] Two-layered tissue-engineered urethra using oral epithelial and muscle-derived cells. (Symposium presentation) (2011)
  • Author(s): Mikami H., Kuwahara G., Nakamura N., Yamato M., Tanaka M., *Kodama S.(*Correspondence)
  • Organizer: Tissue Engineering & Regenerative Medicine International Society(TERMIS) -EU
  • Place of Presentation: Granada, Spain
• [Patent(Industrial Property Rights)] 抗アポトーシス又は抗ネクロシース誘導方法
  • Inventor(s): 小玉 正太
  • Industrial Property Rights Holder: 小玉 正太
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-553616
  • Overseas