How do vascular endothelial cells ameliorate white matter damage?

• Project/Area Number: 23650159
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: Gunma University
• Principal Investigator: ISHIZAKI Yasuki 群馬大学, 大学院・医学系研究科, 教授 (90183003)
• Co-Investigator(Renkei-kenkyūsha): SHIBASAKI Koji 群馬大学, 大学院・医学系研究科, 准教授 (20399554) ; IMAI Hideaki 東京大学, 医学部附属病院, 特任講師 (70359587) ; NARUSE Masae 群馬大学, 大学院・医学系研究科, 助教 (60455219)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 移植・再生医療 / 神経科学 / 脳神経疾患 / 再生医学 / 細胞・組織 / 虚血 / 白質梗塞 / 内皮細胞 / オリゴデンドロサイト / 再生 / 脱随 / 前駆細胞

Research Abstract

I found that transplantation of cerebral microvascular endothelial cells ameliorates white matter damage. I also found that cerebral microvascular endothelial cells stimulate proliferation of oligodendrocyte precursor cells in vitro. The conditioned medium from microvascular endothelial cell culture also stimulates proliferation of oligodendrocyte precursor cells, indicating that a humoral factor produced and released by endothelial cells acts as a mitogen for oligodendrocyte precursor cells.

Research Products

• [Journal Article] DynamicChanges of CD44 Expression from Progenitors to Subpopulations of Astrocytes and Neurons in Developing Cerebellum (2013)
  • Author(s): Naruse, M., Shibasaki, K., Yokoyama, S., Kurachi, M., Ishizaki, Y.
  • Journal Title: PloS one Volume: 8
  • Peer Reviewed
• [Journal Article] Dynamic Changes of CD44 Expression from Progenitors to Subpopulations of Astrocytes and Neurons in Developing Cerebellum. (2013)
  • Author(s): Masae Naruse, Koji Shibasaki, Shuichi Yokoyama, Masashi Kurachi, Yasuki Ishizaki
  • Journal Title: PloS one Volume: 8 Issue: 1 Pages: e53109-e53109
  • DOI: 10.1371/journal.pone.0053109
  • Peer Reviewed
• [Journal Article] Localization of acetylcholine-related molecules in the retina: implication of the communication from photoreceptor to retinal pigment epithelium (2012)
  • Author(s): Matsumoto, H., Shibasaki, K., Uchigashima, M., Koizumi, A., Kurachi, M., Moriwaki, Y., Misawa, H., Kawashima, K., Watanabe, M., Kishi, S., Ishizaki, Y.
  • Journal Title: PloS one Volume: 7
  • Peer Reviewed
• [Journal Article] Brain microvascular endothelial celltransplantation ameliorates ischemic white matter damage (2012)
  • Author(s): Puentes, S., Kurachi, M., Shibasaki, K., Naruse, M., Yoshimoto, Y., Mikuni, M., Imai, H., Ishizaki, Y.
  • Journal Title: Brain Res Volume: 1469 Pages: 43-53
  • Peer Reviewed
• [Journal Article] Methionine excess in diet induces acute lethal hepatitis in mice lacking cystathionine gamma-lyase, an animal model of cystathioninuria. Free Radic (2012)
  • Author(s): Yamada, H., Akahoshi, N., Kamata, S., Hagiya, Y., Hishiki, T., Nagahata, Y., Matsuura, T., Takano, N., Mori, M., Ishizaki, Y., Izumi, T., Kumagai, Y., Kasahara, T., Suematsu, M., Ishii, I.
  • Journal Title: Biol. Med Volume: 52 Pages: 1716-1726
  • Peer Reviewed
• [Journal Article] CD44-positive cells are candidates forastrocyte precursor cells in developing mouse cerebellum (2012)
  • Author(s): Cai, N., Kurachi, M., Shibasaki, K., Okano-Uchida, T., Ishizaki, Y.
  • Journal Title: Cerebellum Volume: 11 Pages: 181-193
  • Peer Reviewed
• [Journal Article] Methionine excess in diet induces acute lethal hepatitis in mice lacking cystathionine gamma-lyase, an animal model of cystathioninuria. (2012)
  • Author(s): Yamada H, Akahoshi N, Kamata S, Hagiya Y, Hishiki T, Nagahata Y, Matsuura T, Takano N, Mori M, Ishizaki Y, Izumi T, Kumagai Y, Kasahara T, Suematsu M, Ishii I
  • Journal Title: Free Radic Biol Med Volume: 52 Issue: 9 Pages: 1716-1726
  • DOI: 10.1016/j.freeradbiomed.2012.02.033
  • Peer Reviewed
• [Journal Article] Brain microvascular endothelial cell transplantation ameliorates ischemicwhite matter damage. (2012)
  • Author(s): Puentes S, Kurachi M, Shibasaki K, Naruse M, Yoshimoto Y, Mikuni M, Imai H, Ishizaki Y.
  • Journal Title: Brain Res Volume: 1469 Pages: 43-53
  • DOI: 10.1016/j.brainres.2012.06.042
  • Peer Reviewed
• [Journal Article] Localization of acetylcholine-related molecules in the retina: implication of the communication from photoreceptor to retinal pigment epithelium. (2012)
  • Author(s): Hidetaka Matsumoto
  • Journal Title: PloS one Volume: 7 Issue: 8 Pages: e42841-e42841
  • DOI: 10.1371/journal.pone.0042841
  • Peer Reviewed
• [Journal Article] Cd44-Positive Cells Are Candidates for Astrocyte Precursor Cells in Developing Mouse Cerebellum. (2012)
  • Author(s): Na Cai, Masashi Kurachi, Koji Shibasaki, Takayuki Okano-Uchida, Yasuki Ishizaki
  • Journal Title: Cerebellum Volume: 11 Issue: 1 Pages: 181-193
  • DOI: 10.1007/s12311-011-0294-x
  • Peer Reviewed
• [Presentation] 脳微小血管内皮細胞移植は白質梗塞巣における再髄鞘化を促進する (2012)
  • Author(s): 石崎泰樹
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-15
• [Presentation] 脳微小血管内皮細胞移植は白質梗塞巣における再髄鞘化を促進する (2012)
  • Author(s): 石崎泰樹
  • Organizer: 第８５回日本生化学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Astrocyte precursor cells in the developing mouse cerebellum (2011)
  • Author(s): Ishizaki, Y.
  • Organizer: the 23^<rd> Biennial Joint Meeting of the International Society for Neurochemistry (ISN) and the European Society for Neurochemistry (ESN)
  • Place of Presentation: アテネ国際会議場(アテネ、ギリシア)
  • Year and Date: 2011-08-29
• [Presentation] ASTROCYTE PRECURSOR CELLS IN THE DEVELOPING MOUSE CEREBELLUM (2011)
  • Author(s): Yasuki Ishizaki, Na Cai, Masashi Kurachi, Koji Shibasaki, Takayuki Okano-Uchida
  • Organizer: the 23rd Biennial Joint Meeting of the International Society for Neurochemistry (ISN) and the European Society for Neurochemistry (ESN)
  • Place of Presentation: Athens, Greece
• [Remarks]
  • URL: http://www.med.gunma-u.ac.jp/med-organization/neurosci/neurosci-development/109.html/