Real-time and multi-color imaging of synapse elimination in the CNS.

• Project/Area Number: 23650160
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: The University of Tokyo
• Principal Investigator: UESAKA Naofumi 東京大学, 大学院・医学系研究科, 助教 (70597624)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: シナプス刈り込み / 小脳 / プルキンエ細胞 / 登上線維 / 蛍光タンパク質 / レンチウィルス / イメージング / リアルタイムイメージング / マルチカラー / 蛍光タンパク

Research Abstract

During brain development, immature redundant neural circuitry is refined into functionally mature versions through the process known as “synapse elimination”. However, how synapse elimination proceeds is largely unknown. In this study, we have developed the method for multi-color labeling of cells and synapses by various fluorescent proteins like green fluorescent protein(GFP), and real-time imaging of these cells and synapses.

Research Products

• [Journal Article] Organotypic coculture preparation for the study of developmental synapse elimination in mammalian brain (2012)
  • Author(s): Uesaka N, Mikuni T, Hashimoto K, Hirai H, Sakimura K, Kano M
  • Journal Title: J. Neurosci Volume: 32(34) Pages: 11657-11670
  • Peer Reviewed
• [Journal Article] Synapse type-independent degradation of the endocannabinoid 2-arachidonoylglycerol after retrograde synaptic suppression (2012)
  • Author(s): Tanimura A, Uchigashima M, Yamazaki M, Uesaka N, Mikuni T, Manabu Abe, Kouichi Hashimoto, Masahiko Watanabe, Kenji Sakimura and Masanobu Kano
  • Journal Title: Proc Natl Acad Sci U S A
  • Peer Reviewed
• [Journal Article] Organotypic coculture preparation for the study of developmental synapse elimination in mammalian brain (2012)
  • Author(s): Uesaka N
  • Journal Title: J Neurosci Volume: 32 Issue: 34 Pages: 11688-11699
  • DOI: 10.1523/jneurosci.1097-12.2012
  • Peer Reviewed
• [Journal Article] Synapse type-independent degradation of the endocannabinoid 2-arachidonoylglycerol after retrograde synaptic suppression (2012)
  • Author(s): Tanimura A
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Issue: 30 Pages: 12195-12200
  • DOI: 10.1073/pnas.1204404109
  • Peer Reviewed
• [Presentation] 組織培養法と細胞種特異的遺伝子導入法を用いたシナプス刈り込みのメカニズムの解明 (2013)
  • Author(s): 上阪直史
  • Organizer: 第90回(平成24年度)日本生理学会
  • Place of Presentation: 東京
• [Presentation] 組織培養法と細胞種特異的遺伝子導入法を用いたシナプス刈り込みのメカニズムの解明 (2013)
  • Author(s): 上阪 直史
  • Organizer: 第90回日本生理学会
  • Place of Presentation: 東京・タワーホール船堀
  • Invited
• [Presentation] Possible involvement of the Ca2+-dependent gene Arc in activity-dependent synapse elimination in the cerebellum (2012)
  • Author(s): 三國貴康
  • Organizer: 北米神経科学学会Neuroscience2012
  • Place of Presentation: アメリカニューオーリンズ
  • Year and Date: 2012-10-05
• [Presentation] Synapse type-independent degradation of 2-arachidonoylglycerol after retrograde synaptic suppression in the cerebellum (2012)
  • Author(s): 谷村あさみ
  • Organizer: 北米神経科学学会Neuroscience2012
  • Place of Presentation: アメリカ ニューオーリンズ
  • Year and Date: 2012-10-05
• [Presentation] Possible involvement of the Ca2+-dependent gene Arc in activity-dependent synapse elimination in the cerebellum (2012)
  • Author(s): Takayasu Mikuni
  • Organizer: Neuroscience 2012
  • Place of Presentation: USA・New Orleans
• [Presentation] Synapse type-independent degradation of 2-arachidonoylglycerol after retrograde synaptic suppression in the cerebellum (2012)
  • Author(s): Asami Tanimura
  • Organizer: Neuroscience 2012
  • Place of Presentation: USA・New Orleans
• [Presentation] Postsynaptic Purkinje cell activity accelerates climbing fiber synapse elimination in an organotypic coculture preparation of the cerebellum and medulla oblongata (2011)
  • Author(s): 三國貴康
  • Organizer: 北米神経科学学会Neuroscience2011
  • Place of Presentation: アメリカワシントン
• [Presentation] 小脳-延髄共培養標本の登上線維シナプス除去におけるプルキンエ細胞活動の役割 (2011)
  • Author(s): 三國貴康
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、神奈川
• [Presentation] 2-アラキドノイルグリセロールリパーゼは小脳皮質においてシナプス非特異的に分解される (2011)
  • Author(s): 谷村あさみ
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、神奈川
• [Presentation] 2-アラキドノイルグリセロールリパーゼは小脳皮質においてシナプス非特異的に 分解される (2011)
  • Author(s): 谷村 あさみ, 山崎 真弥, 内が島 基政, 上阪 直史, 三国 貴康, 橋本 浩一, 渡辺 雅彦, 崎村 建二, 狩野 方伸
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、神奈川
• [Presentation] Postsynaptic Purkinje cell activity accelerates climbing fiber synapse elimination in an organotypic coculture preparation of the cerebellum and medulla oblongata (2011)
  • Author(s): 三國 貴康, 上阪 直史, 狩野 方伸
  • Organizer: Neuroscience 2011
  • Place of Presentation: ワシントン、米国
• [Remarks]
  • URL: http://plaza.umin.ac.jp/?neurophy/Kano_Lab_j/Top_j.html
• [Remarks] 東京大学大学院医学系研究科神経生理学教室
  • URL: http://plaza.umin.ac.jp/~neurophy/Kano_Lab_j/Top_j.html