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Real-time and multi-color imaging of synapse elimination in the CNS.

Research Project

Project/Area Number 23650160
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neuroscience in general
Research InstitutionThe University of Tokyo

Principal Investigator

UESAKA Naofumi  東京大学, 大学院・医学系研究科, 助教 (70597624)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsシナプス刈り込み / 小脳 / プルキンエ細胞 / 登上線維 / 蛍光タンパク質 / レンチウィルス / イメージング / リアルタイムイメージング / マルチカラー / 蛍光タンパク
Research Abstract

During brain development, immature redundant neural circuitry is refined into functionally mature versions through the process known as “synapse elimination”. However, how synapse elimination proceeds is largely unknown. In this study, we have developed the method for multi-color labeling of cells and synapses by various fluorescent proteins like green fluorescent protein(GFP), and real-time imaging of these cells and synapses.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (17 results)

All 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (11 results) (of which Invited: 1 results) Remarks (2 results)

  • [Journal Article] Organotypic coculture preparation for the study of developmental synapse elimination in mammalian brain2012

    • Author(s)
      Uesaka N, Mikuni T, Hashimoto K, Hirai H, Sakimura K, Kano M
    • Journal Title

      J. Neurosci

      Volume: 32(34) Pages: 11657-11670

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synapse type-independent degradation of the endocannabinoid 2-arachidonoylglycerol after retrograde synaptic suppression2012

    • Author(s)
      Tanimura A, Uchigashima M, Yamazaki M, Uesaka N, Mikuni T, Manabu Abe, Kouichi Hashimoto, Masahiko Watanabe, Kenji Sakimura and Masanobu Kano
    • Journal Title

      Proc Natl Acad Sci U S A

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Organotypic coculture preparation for the study of developmental synapse elimination in mammalian brain2012

    • Author(s)
      Uesaka N
    • Journal Title

      J Neurosci

      Volume: 32 Issue: 34 Pages: 11657

    • DOI

      10.1523/jneurosci.1097-12.2012

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synapse type-independent degradation of the endocannabinoid 2-arachidonoylglycerol after retrograde synaptic suppression2012

    • Author(s)
      Tanimura A
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 109 Issue: 30 Pages: 12195

    • DOI

      10.1073/pnas.1204404109

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 組織培養法と細胞種特異的遺伝子導入法を用いたシナプス刈り込みのメカニズムの解明2013

    • Author(s)
      上阪直史
    • Organizer
      第90回(平成24年度)日本生理学会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] 組織培養法と細胞種特異的遺伝子導入法を用いたシナプス刈り込みのメカニズムの解明2013

    • Author(s)
      上阪 直史
    • Organizer
      第90回日本生理学会
    • Place of Presentation
      東京・タワーホール船堀
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Possible involvement of the Ca2+-dependent gene Arc in activity-dependent synapse elimination in the cerebellum2012

    • Author(s)
      三國貴康
    • Organizer
      北米神経科学学会Neuroscience2012
    • Place of Presentation
      アメリカニューオーリンズ
    • Year and Date
      2012-10-05
    • Related Report
      2012 Final Research Report
  • [Presentation] Synapse type-independent degradation of 2-arachidonoylglycerol after retrograde synaptic suppression in the cerebellum2012

    • Author(s)
      谷村あさみ
    • Organizer
      北米神経科学学会Neuroscience2012
    • Place of Presentation
      アメリカ ニューオーリンズ
    • Year and Date
      2012-10-05
    • Related Report
      2012 Final Research Report
  • [Presentation] Possible involvement of the Ca2+-dependent gene Arc in activity-dependent synapse elimination in the cerebellum2012

    • Author(s)
      Takayasu Mikuni
    • Organizer
      Neuroscience 2012
    • Place of Presentation
      USA・New Orleans
    • Related Report
      2012 Annual Research Report
  • [Presentation] Synapse type-independent degradation of 2-arachidonoylglycerol after retrograde synaptic suppression in the cerebellum2012

    • Author(s)
      Asami Tanimura
    • Organizer
      Neuroscience 2012
    • Place of Presentation
      USA・New Orleans
    • Related Report
      2012 Annual Research Report
  • [Presentation] Postsynaptic Purkinje cell activity accelerates climbing fiber synapse elimination in an organotypic coculture preparation of the cerebellum and medulla oblongata2011

    • Author(s)
      三國貴康
    • Organizer
      北米神経科学学会Neuroscience2011
    • Place of Presentation
      アメリカワシントン
    • Related Report
      2012 Final Research Report
  • [Presentation] 小脳-延髄共培養標本の登上線維シナプス除去におけるプルキンエ細胞活動の役割2011

    • Author(s)
      三國貴康
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜、神奈川
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] 2-アラキドノイルグリセロールリパーゼは小脳皮質においてシナプス非特異的に分解される2011

    • Author(s)
      谷村あさみ
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜、神奈川
    • Related Report
      2012 Final Research Report
  • [Presentation] 2-アラキドノイルグリセロールリパーゼは小脳皮質においてシナプス非特異的に 分解される2011

    • Author(s)
      谷村 あさみ, 山崎 真弥, 内が島 基政, 上阪 直史, 三国 貴康, 橋本 浩一, 渡辺 雅彦, 崎村 建二, 狩野 方伸
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜、神奈川
    • Related Report
      2011 Research-status Report
  • [Presentation] Postsynaptic Purkinje cell activity accelerates climbing fiber synapse elimination in an organotypic coculture preparation of the cerebellum and medulla oblongata2011

    • Author(s)
      三國 貴康, 上阪 直史, 狩野 方伸
    • Organizer
      Neuroscience 2011
    • Place of Presentation
      ワシントン、米国
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://plaza.umin.ac.jp/?neurophy/Kano_Lab_j/Top_j.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 東京大学大学院医学系研究科神経生理学教室

    • URL

      http://plaza.umin.ac.jp/~neurophy/Kano_Lab_j/Top_j.html

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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