Live imaging of energy supply for synaptic protein transport
| Project/Area Number |
23650169
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neuroscience in general
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 分子 / 細胞神経科学 / ミトコンドリア / 軸索輸送 / シナプス / ＡＴＰ / 蛍光蛋白 / イメージング / シナプス前終末 / 蛋白 / イメ－ジング |
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| Research Abstract |
Preparing synaptic contacts in developing (three weeks in culture) and mature (6 weeks in culture) superior cervical ganglion (SCG) neurons, I performed live image of axonal transport of CFP-labeled mitochondria, RFP-labeled synaptophysin,a synaptic vesicle protein, and GFP-labeled CAST, an active zone protein, applying variousaction potential firing pattern to a presynaptic neuron. Their conduction velocity in the axon and their accumulation in the axon or into the terminal were impaired with dysfunction of a motor adaptor protein.
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Report
(3 results)
Research Products
(34 results)
