| Project/Area Number |
23650175
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIOKI Hiroyuki 京都大学, 医学(系)研究科(研究院), 助教 (00402850)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | レンチウイルス / アデノ随伴ウイルス / 神経細胞 / 局所神経回路 / 定量的解析 / ウイルスベクター / 遺伝子改変動物 / 神経シナプストレーサー / 経シナプストレーサー |
|---|
| Research Abstract |
1In the present study, we developed viral vectors, which express transsynaptic tracer protein, tetanus toxin C fragment (TTC) or wheat germ agglutinin (WGA). We then injected the viral vectors into the neocortex of the wild rats or Cre Tg mice, and observed the immunoreactivity for TTC or WGA. We could observe the transsynaptic transfer of TTC or WGA, but unfortunately the signal intensities were too week to analyze the cell types and numbers even with high-sensitive detection method.
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