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Development of screening method to isolate molecules that regulate behaviors of neurotransmitter receptors at synaptic membranes

Research Project

Project/Area Number 23650180
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Nerve anatomy/Neuropathology
Research InstitutionNational Institute for Physiological Sciences

Principal Investigator

TABUCHI Katsuhiko  生理学研究所, 大脳皮質機能研究系, 教授(兼任) (20546767)

Co-Investigator(Renkei-kenkyūsha) EGASHIRA Yoshihiro  同志社大学, 脳科学研究科, 研究員 (80582410)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords神経実験形態学 / シナプス / 凍結割断レプリカ免疫電顕 / 子宮内エレクトロポレーション / 子宮内エレクトロポレーション法 / SDS-FRL法
Research Abstract

SDS-digested freeze-fracture replica labeling electronmiroscopy (SDS-FRL EM) is a technique that enables visualization of proteins, such as neurotransmitter receptors, on synapse membrane with maintenance of space structure. However, the technique in combination with gene transfection has not been established because of the difficulty in recognition of transfected neurons. In this project, we developed a new technique to distinguish transfected neurons on replica membranes by refolding membrane tethered fluorescent proteins that are introduced as markers after SDS digestion.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (23 results)

All 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 7 results) Presentation (9 results) (of which Invited: 4 results) Remarks (4 results)

  • [Journal Article]2013

    • Author(s)
      Elaine C. Budreck, Oh-Bin Kwon, Jung Hoon Jung, Stephane Baudouin, Albert Thommen, Hye-Sun Kim, Yugo Fukazawa, Harumi Harada, Katsuhiko Tabuchi, Ryuichi Shigemoto, Peter Scheiffele
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 110(2) Pages: 725-730

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neuroligin-1 controls synaptic abundance of NMDA-type glutamate receptors through extracellular coupling2013

    • Author(s)
      Fukazawa Y(他10名)
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 110 Issue: 2 Pages: 725-730

    • DOI

      10.1073/pnas.1214718110

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of Neuroligin-3 R451C knock-in mice as models for autistic savant2012

    • Author(s)
      Deeba Noreen Baig, Toru Yanagawa, Katsuhiko Tabuchi
    • Journal Title

      Japanese Journal of Biological Psychiatry

      Volume: 23(4) Pages: 281-286

    • NAID

      130005395429

    • Related Report
      2012 Final Research Report
  • [Journal Article] Analysis of Neuroligin-3 R451C knock-in mice as models for autistic savant.2012

    • Author(s)
      Deeba Noreen Baig
    • Journal Title

      Japanese Journal of Biological Psychiatry

      Volume: 23(4) Pages: 281-286

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Autism-linked Neuroligin-3 R451C mutation differentially alters hippocampal and cortical synaptic function2011

    • Author(s)
      Mark Etherton, Csaba Foldy, Manu Sharma, Katsuhiko Tabuchi, Xinran Liu, Mehrdad Shamloo, Robert C, Malenka, Thomas C Sudhof
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 108(33) Pages: 13764-13769

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] An autism-associated point mutation in the Neuroligin cytoplasmic tail selectively impairs AMPA receptor-mediated synaptic transmission in hippocampus2011

    • Author(s)
      Mark R Etherton, Katsuhiko Tabuchi, Manu Sharma, Jaewon Ko, Thomas C Sudhof
    • Journal Title

      EMBO-J

      Volume: 30(14) Pages: 2908-2919

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neuroligin2011

    • Author(s)
      田渕克彦
    • Journal Title

      分子精神医学

      Volume: 11(3) Pages: 207-218

    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Journal Article] Autism-linked neuroligin-3 R451C mutation differentially alters hippocampal and cortical synaptic function.2011

    • Author(s)
      Mark Etherton, Csaba Foldy, Manu Sharma, Katsuhiko Tabuchi, Xinran Liu, Mehrdad Shamloo, Robert C. Malenka, Thomas C. Sudhof
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 10 L8(33) Pages: 13764-13769

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] An autism-associated point mutation in the neuroligin cytoplasmic tail selectively impairs AMPA receptor-mediated synaptic transmission in hippocampus.2011

    • Author(s)
      Mark R Etherton, Katsuhiko Tabuchi, Manu Sharma, Jaewon Ko and Thomas C Sudhof
    • Journal Title

      EMBO-J

      Volume: 30(14) Pages: 2908-2919

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Presynaptic Neurexin-3 Alternative Splicing Trans-Synaptically Controls Postsynaptic AMPA-Receptor Traficking

    • Author(s)
      Jason Aoto, David C Martinelli, Robert C Malenka, Katsuhiko Tabuchi, Thomas C. Sudhof
    • Journal Title

      Cell

      Volume: (in press)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Synapse maturation and autism: The role of synapse adhesion molecules2013

    • Author(s)
      田渕克彦,張文欣,Nur Farehan Mohamed Asgar, Thomas C Sudhof, 重本隆一
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Year and Date
      2013-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] Synapse maturation and autism: The role of synapse adhesion molecules2013

    • Author(s)
      田渕 克彦
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Synapse maturation and autism: The role of Neuroligins and Neurexins2012

    • Author(s)
      Katsuhiko Tabuchi
    • Organizer
      The 11the Biennial meeting of the Asia Pacific Society for Neurochemistry
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2012-10-01
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of Neuroligin mouse models relevant to pathophysiology of autism2012

    • Author(s)
      田渕克彦
    • Organizer
      第34回日本生物学的精神医学会
    • Place of Presentation
      日神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2012-09-30
    • Related Report
      2012 Final Research Report
  • [Presentation] Synapse function and autism: The role of Neuroligins and Neurexins2012

    • Author(s)
      Katsuhiko Tabuchi
    • Organizer
      15t^<th> Annual Neuroscience Symposium The Korean Society for Brain and Neural Science
    • Place of Presentation
      ソウル国立大学(韓国)
    • Year and Date
      2012-09-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Synapse function and autism: The role of Neuroligins and Neurexins2012

    • Author(s)
      Katsuhiko Tabuchi
    • Organizer
      15th Annual Neuroscience Symposium The Korean Society for Brain and Neural Science
    • Place of Presentation
      ソウル国立大学(韓国)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Analysis of Neuroligin mouse models relevant to pathophysiology of autism.2012

    • Author(s)
      田渕 克彦
    • Organizer
      第34回日本生物学的精神医学会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Synapse maturation and autism: The role of Neuroligins and Neurexins2012

    • Author(s)
      Katsuhiko Tabuchi
    • Organizer
      The 11th Biennial Meeting of the Asia Pacific Society for Neurochemistry
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 自閉症関連シナプス接着因子の変異が神経伝達に及ぼす影響2011

    • Author(s)
      田渕克彦
    • Organizer
      こころの発達と傷害の教育研究コンソーシアム第2回シンポジウム(招待講演)
    • Place of Presentation
      東京大学鉄門記念講堂(東京都)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.shinshu-u.ac.jp/faculty/medicine/chair/i-2seiri/ja/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 自然科学研究機構生理学研究所田渕克彦

    • URL

      http://www.nips.ac.jp/dcs/member_tabuchi.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://katsuhikotabuchi.web.fc2.com/

    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.nips.ac.jp/dcs/member_tabuchi.html

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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