Establishment of the diagnostics of Alzheimer' s disease using A・43

• Project/Area Number: 23650185
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SAITO Takashi 独立行政法人理化学研究所, 神経蛋白制御研究チーム, 副チームリーダー (90360552)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: アルツハイマー病 / トランスレーショナルリサーチ / 早期診断

Research Abstract

Establishment of an early diagnosis for Alzheimer’s disease (AD) is one of the important missions in an aging society. Recently, we found that Ass43 is generated under an age-dependent manner, thereby it might be a good candidate as a marker for an early diagnosis of AD. Herein, we established a high-sensitive and quantitative method to detect Ass43 specifically in the sample from mice and human materials. Finally, we concluded that Ass43 has a potent pathogenicity, and would be an useful candidate for an early diagnosis for AD.

Research Products

• [Journal Article] Potent amyloidogenicity and pathogenicity of Ass43 (2011)
  • Author(s): Takashi Saito, Takahiro Suemoto, Nathalie Brouwers, Kristel Sleegers, Satoru Funamoto, Naomi Mihira, Yukio Matsuba, Kazuyuki Yamada, Per Nilsson, Jiro Takano, Masaki Nishimura, Nobuhisa Iwata, Christine V. Broeckhoven, Yasuo Ihara, Takaomi C. Saido
  • Journal Title: Nature Neuroscience 14 Pages: 1023-1032
• [Journal Article] Potent amyloidgenecity and pathogenicity of Aβ43 (2011)
  • Author(s): Saito T, Suemoto T, Brouwers N, Sleegers K, Funamoto S, Mihira N, Matsuba Y, Yamada K, Nilsson P, Takano J, Nishimura M, Iwata N, Van Broeckhoven C, Ihara Y, Saido TC
  • Journal Title: Nature Neuroscience Volume: 14 Pages: 1023-1032
  • Peer Reviewed
• [Presentation] The role of intracellular domain in neprilysin for the metabolic pathway and enzymatic activity of neprilysin (2012)
  • Author(s): 垣矢直雅、斉藤貴志、Per Nilsson、松葉由紀夫、津吹聡、西道隆臣
  • Organizer: 日本生化学会(ポスター発表)
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-15
• [Presentation] アミロイドss43によるアルツハイマー病の病態発症・促進機構に関する研究 (2012)
  • Author(s): 斉藤 貴志
  • Organizer: 日本生化学会(日本生化学会奨励賞 受賞講演)
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-14
• [Presentation] 新規APPノックインマウスの作製と応用 (2012)
  • Author(s): 斉藤 貴志、松葉 由紀夫、三平 尚美、岩田 修永、高野 二郎、Per Nilsson、西道 隆臣
  • Organizer: 日本認知症学会学術集会(口頭・ポスター発表)
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2012-10-26
• [Presentation] Novel type and more relevant model mouse for Alzheimer's disease (2012)
  • Author(s): Takashi Saito, Yukio Matsuba, Naomi Mihira, Nobuhisa Iwata, Jiro Takano, Per Nilsson and Takaomi C. Saido
  • Organizer: Annual meeting of Society for Neuroscience 2012(ポスター発表)
  • Place of Presentation: ニューオリンズ(アメリカ)
  • Year and Date: 2012-10-15
• [Presentation] APP knock-in mouse: a novel and more relevant model mouse for Alzheimer's disease (2012)
  • Author(s): Takashi Saito, Yukio Matsuba, Naomi Mihira, Nobuhisa Iwata, Jiro Takano, Per Nilsson and Takaomi C. Saido
  • Organizer: Alzheimer's Association International Conference 2012(ポスター発表)
  • Place of Presentation: バンクーバー(カナダ)
  • Year and Date: 2012-07-15
• [Presentation] Analysis of the regulatory mechanisms of neprilysin activity (2011)
  • Author(s): 垣矢直雅、斉藤貴志、津吹聡、西道隆臣
  • Organizer: 日本生化学会(ポスター発表)
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2011-09-24
• [Presentation] Analysis of the regulatory mechanisms of neprilysin activity (2011)
  • Author(s): Naomasa Kakiya, Takashi Saito, Satoshi Tsubuki, Takaomi C. Saido
  • Organizer: 日本生化学会
  • Place of Presentation: 京都国際会議場
• [Presentation] アミロイドβ43によるアルツハイマー病の病態発症・促進機構に関する研究
  • Author(s): 斉藤貴志
  • Organizer: 日本生化学会
  • Place of Presentation: 福岡国際会議場
  • Invited
• [Presentation] The role of intracellular domain in neprilysin for the metabolic pathway and enzymatic activity of neprilysin
  • Author(s): 垣矢直雅、斉藤貴志、Per Nilsson、松葉由紀夫、津吹聡、西道隆臣
  • Organizer: 日本生化学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 新規APPノックインマウスの作製と応用
  • Author(s): 斉藤貴志、松葉由紀夫、三平尚美、岩田修永、高野二郎、Per Nilsson、西道隆臣
  • Organizer: 日本認知症学会
  • Place of Presentation: つくば国際会議場
• [Presentation] Novel type and more relevant model mouse for Alzheimer's disease
  • Author(s): 斉藤貴志、松葉由紀夫、三平尚美、岩田修永、高野二郎、Per Nilsson、西道隆臣
  • Organizer: Annual meeting of Society for Neuroscience
  • Place of Presentation: ニューオリンズ（アメリカ）
• [Presentation] APP knock-in mouse: a novel and more relevant model mouse for Alzheimer's disease
  • Author(s): 斉藤貴志、松葉由紀夫、三平尚美、岩田修永、高野二郎、Per Nilsson、西道隆臣
  • Organizer: Alzheimer's Association International Conference 2012
  • Place of Presentation: バンクーバー（カナダ）
• [Remarks] 所属研究室のホームページ
  • URL: http://www.brain.riken.jp/labs/pns/
• [Remarks] 平成24年度 日本生化学会奨励賞、および平成25年度 文部科学大臣表彰 若手科学者賞を受賞した。 受賞
• [Remarks] アルツハイマー病の重要な原因物質としてAβ43が浮上
  • URL: http://www.riken.go.jp/pr/press/2011/20110704/