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Analysis of non-coding RNAs regulating neural differentiation of human ES cells.

Research Project

Project/Area Number 23650194
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurochemistry/Neuropharmacology
Research InstitutionKeio University

Principal Investigator

OKADA Yohei  慶應義塾大学, 医学部, 特任講師 (30383714)

Co-Investigator(Renkei-kenkyūsha) TSUNODA Tatsuhiko  理化学研究所, ゲノム医科学研究センター, チームリーダー (10273468)
MIYA Fuyuki  理化学研究所, ゲノム医科学研究センター, 研究員 (50415311)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsヒトES細胞 / ノンコーディングRNA / ノンコーディングRAN / 神経幹細胞 / 神経分化 / ncRNA
Research Abstract

Several non-coding RNAs (ncRNAs) upregulated during neural differentiation of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) were identified by genome-wide transcriptome analysis. In addition, methods for the analysis of iden

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (46 results)

All 2013 2012 2011 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (25 results) (of which Invited: 1 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Steps toward safe cell therapy using induced pluripotent stem cells2013

    • Author(s)
      Okano H
    • Journal Title

      Circulation Research

      Volume: 112 Issue: 3 Pages: 523-33

    • DOI

      10.1161/circresaha.111.256149

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced aggregation of androgen receptor in induced pluripotent stem cell-derived neurons from spinal and bulbar muscular atrophy.2013

    • Author(s)
      Nihei Y, Ito D, Okada Y, Akamatsu W, Yagi T, Yoshizaki T, Okano H, Suzuki N.
    • Journal Title

      J Biol Chem.

      Volume: 288 Issue: 12 Pages: 8043-8052

    • DOI

      10.1074/jbc.m112.408211

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pre-Evaluated Safe Human iPSC-Derived Neural Stem Cells Promote Functional Recovery after Spinal Cord Injury in Common Marmoset without Tumorigenicity2012

    • Author(s)
      Kobayashi Y, Okada Y
    • Journal Title

      PLoS One

      Volume: 7 Issue: 12 Pages: e52787-e52787

    • DOI

      10.1371/journal.pone.0052787

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiple analyses of G-protein coupled receptor (GPCR) expression in the development of gefitinib-resistance in transforming non-small-cell lung cancer.2012

    • Author(s)
      Kuzumaki N
    • Journal Title

      PLoS One

      Volume: 7 Issue: 10 Pages: e44368-e44368

    • DOI

      10.1371/journal.pone.0044368

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficient Derivation of Multipotent Neural Stem/Progenitor Cells from Non-human Primate Embryonic Stem Cells2012

    • Author(s)
      Shimada H, Okada Y, Ibata K, Ebise H, Ota S, Tomioka I, Nomura T, Maeda T, Kohda K, Yuzaki M, Sasaki E, Nakamura M, Okano H
    • Journal Title

      PLoS One

      Volume: 7(11) Issue: 11 Pages: e49469-e49469

    • DOI

      10.1371/journal.pone.0049469

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial dysfunction associated with increased oxidative stress and -synuclein accumulation in PARK2 iPSC-derived neurons and postmortem brain tissue.2012

    • Author(s)
      Imaizumi Y
    • Journal Title

      Mol Brain

      Volume: 5 Issue: 1 Pages: 35-35

    • DOI

      10.1186/1756-6606-5-35

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of induced pluripotent stem cells from centenarians for neurodegenerative disease research2012

    • Author(s)
      Yagi, T.
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 7 Pages: e41572-e41572

    • DOI

      10.1371/journal.pone.0041572

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] RNA-Binding Protein Musashi1 Modulates Glioma Cell Growth through the Post-Transcriptional Regulation of Notch and PI(3) Kinase/Akt Signaling Pathways2012

    • Author(s)
      Muto J, Imai T, Ogawa D, Nishimoto Y, Okada Y, Mabuchi Y, Kawase T, Iwanami A, Mischel PS, Saya H, Yoshida K, Matsuzaki Y, Okano H
    • Journal Title

      PLoS One

      Volume: 7(3) Issue: 3 Pages: e33431-e33431

    • DOI

      10.1371/journal.pone.0033431

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of k-opioid receptor agonist on the growth of non-small cell lung cancer (NSCLC) cells2012

    • Author(s)
      Kuzumaki N, Suzuki A, Narita M, Hosoya T, Nagasawa A, Imai S, Yamamizu K, Morita H, Nagase H, Okada Y, Okano HJ, Yamashita JK, Okano H, Suzuki T, Narita M
    • Journal Title

      Br J Cancer

      Volume: 106(Epub 2012 Feb 16) Issue: 6 Pages: 1148-1152

    • DOI

      10.1038/bjc.2011.574

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Steps toward safe cell therapy using induced pluripotent stem cells2012

    • Author(s)
      Okano, H., Nakamura, M., et al
    • Journal Title

      Circulation Research

      Volume: 印刷中

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Grafted human-induced pluripotent stem-cell-derived neurospheres promotemotor functional recovery after spinal cord injury in mice2011

    • Author(s)
      Nori, S., et al
    • Journal Title

      Proceedings of National Academy of Science U.S.A.

      Volume: 108 Issue: 40 Pages: 16825-16830

    • DOI

      10.1073/pnas.1108077108

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Modeling familial Alzheimer's disease with induced pluripotent stem cells2011

    • Author(s)
      Yagi T, Ito D, Okada Y, Akamatsu W, Nihei Y, Yoshizaki T, Yamanaka S, Okano H, Suzuki N
    • Journal Title

      Human Molecular Genetics

      Volume: 20 Issue: 23 Pages: 4530-9

    • DOI

      10.1093/hmg/ddr394

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Musashi 1 cooperates in abnormal cell LINeage protein 28 (Lin28)-mediated Let-7 family microRNA biogenesis in early neural differentiation2011

    • Author(s)
      Kawahara, H., Okada, Y., et al
    • Journal Title

      J Biol Chem

      Volume: 286 Issue: 18 Pages: 16121-30

    • DOI

      10.1074/jbc.m110.199166

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Generation of human melanocytes from induced pluripotent stem cells2011

    • Author(s)
      Ohta S, Imaizumi Y, Okada Y, Akamatsu W, Kuwahara R, Ohyama M, Amagai M, Matsuzaki Y, Yamanaka S, Okano H, Kawakami Y
    • Journal Title

      PLoS One

      Volume: 6(1) Issue: 1 Pages: e16182-e16182

    • DOI

      10.1371/journal.pone.0016182

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene targeting and subsequent site-specific transgenesis at theβ-actin(ACTB) locus in common marmoset embryonic stem cells2011

    • Author(s)
      Shiozawa, S., Suemizu, H.(12名・8番目)
    • Journal Title

      Stem Cells Dev

      Volume: 20 Issue: 9 Pages: 1587-99

    • DOI

      10.1089/scd.2010.0351

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Modeling familial Alzheimer's disease with induced pluripotent stem cells2011

    • Author(s)
      Yagi, T., Ito, D., et al
    • Journal Title

      Hum. Mol. Genet.

      Volume: 20 Pages: 4530-4539

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Human Decidua-Derived Mesenchymal Cells are a Promising Source for the Generation and Cell Banking, of Human Induced Pluripotent Stem Cells Cell Medicine

    • Author(s)
      Shofuda T, Kanematsu D, Fukusumi H, Yamamoto A, Bamba Y, Yoshitatsu S, Suemizu H, Nakamura M, Sugimoto Y, Furue KM, Kohara A, Akamatsu W, Okada Y, Okano H, Yamasaki M, Kanemura Y
    • Journal Title

      Part B of Cell Transplantation

      Volume: (in press)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Incompletely reprogrammed human iPSCs form glioma-like tumors through genomic instability during neural differentiation2013

    • Author(s)
      Okada Y, Miya, F, Koike M, Tomisato S, Tokura T, Ishihara Y, Shimojo D, Hattori C, Kanematsu D, Kanemura Y, Kohda K, Sobue G, Yamanaka S, Yuzaki M, Uchiyama Y, Ikeda E, Tsunoda T, Okano H
    • Organizer
      CiRA International Symoisum 2013, Raising the Nex Generation of Stem Cell Research, Kyoto March 2013, CiRA International Symoisum 2013, Raising the Nex Generation of Stem Cell Research
    • Place of Presentation
      Kyoto
    • Year and Date
      2013-03-12
    • Related Report
      2012 Final Research Report
  • [Presentation] Modeling dysmyelinating neurological disorders using human iPSC-derived mature oligodendrocytes2013

    • Author(s)
      Numasawa Y, Okada Y, Shibata S, Kuzumaki N, Kishi N, Akamatsu W, Amagai M, Osaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H
    • Organizer
      CiRA International Symoisum 2013, Raising the Nex Generation of Stem Cell Research
    • Place of Presentation
      Kyoto
    • Year and Date
      2013-03-11
    • Related Report
      2012 Final Research Report
  • [Presentation] iPS細胞を用いた神経再生と病態解析2013

    • Author(s)
      岡田洋平
    • Organizer
      第4回岐阜脳神経研究会
    • Place of Presentation
      岐阜
    • Year and Date
      2013-02-02
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 不完全にリプログラミングされたヒトiPS細胞由来神経幹細胞は分化誘導に伴うゲノム不安定化によりグリオーマ様腫瘍を形成する2012

    • Author(s)
      岡田洋平、宮冬樹、小池正人、冨里周太、兼松大介、金村米博、幸田和久、柚崎通介、内山安男、池田栄二、角田達彦、山中伸弥、岡野栄之
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-12
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒトiPS細胞由来神経幹細胞の応用とその問題点2012

    • Author(s)
      岡田洋平、岡野栄之
    • Organizer
      第24回日本脳循環代謝学会総会
    • Place of Presentation
      広島
    • Year and Date
      2012-11-09
    • Related Report
      2012 Final Research Report
  • [Presentation] 多能性幹細胞・神経幹細胞を用いた神経再生と病態解析2012

    • Author(s)
      岡田洋平
    • Organizer
      第13回埼玉パーキンソン病治療研究会
    • Place of Presentation
      大宮
    • Year and Date
      2012-11-01
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Evaluation of human iPS cells byneural differentiation and tumorigenicity2012

    • Author(s)
      Okada Y, Miya F, Tomisato S, Kanemura Y, Koike M, Kohda K, Yuzaki M, Uchiyama Y, Tsunoda T, Yamanaka S, Okano H
    • Organizer
      ISSCR 10th annual Meeting
    • Place of Presentation
      Yokohama
    • Year and Date
      2012-06-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Rapid and easy monolayer neural differentiation of human ES cells2012

    • Author(s)
      Ishihara Y, Okada Y, Koakutsu M, Okano H
    • Organizer
      ISSCR 10th annual Meeting
    • Place of Presentation
      Yokohama
    • Year and Date
      2012-06-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Mitochondrial dysfunction and alfa synuclein accumulation in PARK2 IPSC derived neurons and postmortem brain2012

    • Author(s)
      Imaizumi Y, Okada Y, Akamatsu W, Koike M, Hayakawa H, Nihira T, Suematsu M, Suzuki N, Amagai M, Uchiyama Y, Mochizuki H, Hattori N, Okano H
    • Organizer
      ISSCR 10th annual Meeting
    • Place of Presentation
      Yokohama
    • Year and Date
      2012-06-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Exogenous signals oppositely affect adipocyte differentiation and neural sifferentiation of human ES/iPS cells2012

    • Author(s)
      Koakutsu M, Okada Y, Okano H
    • Organizer
      ISSCR 10th annual Meeting
    • Place of Presentation
      Yokohama
    • Year and Date
      2012-06-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Pre-evaluated safe human iPS clone derived neural stem cells promoted functional recovery after spinal cord injury without tumorigenecity in adult common marmosets2012

    • Author(s)
      Kobayashi Y, Okada Y, Iwai H, Nishimura S, Nori S, Konomi T, Fujiyoshi K, Tsuji O, Toyama Y, Yamanaka S, Nakamura M, Okano H
    • Organizer
      ISSCR 10th annual Meeting
    • Place of Presentation
      Yokohama
    • Year and Date
      2012-06-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Efficient derivation of neural stem cells from common marmoset ES cells and iPS cells.2011

    • Author(s)
      Shimada, H., Okada Y., Tomioka, I., Sasaki, E., Nakamura, M., Okano, H.
    • Organizer
      Society for Neuroscience 41th Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2011-11-15
    • Related Report
      2012 Final Research Report
  • [Presentation] 神経分化と造腫瘍性から迫るヒトiPS細胞の品質評価2011

    • Author(s)
      岡田洋平、宮冬樹、 金村米博、 砂堀毅彦、 小池正人、幸田和久、柚崎通介、内山安男、 角田達彦、山中伸弥、岡野栄之
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2011-09-17
    • Related Report
      2012 Final Research Report
  • [Presentation] 神経前駆細胞特異的レポーターヒトES細胞の樹立2011

    • Author(s)
      吉田賢司、太田真一、原央子、岡田洋平、岡野栄之
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2011-09-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Generation and characterization of human ES cells carrying Sox1-reporter gene for neural differentiation.2011

    • Author(s)
      Yoshida, K., Ota, S.-I., Hara, C., Okano, H., Okada Y.
    • Organizer
      8th IBRO World congress of Neuroscience
    • Place of Presentation
      Florence, Italy
    • Year and Date
      2011-07-18
    • Related Report
      2012 Final Research Report
  • [Presentation] Evaluation of human iPS cells by neural differentiation and tumorigenicity2011

    • Author(s)
      Okada Y, Miya F, Kanemura Y, Koike M, Kohda K, Yuzaki M, Uchiyam Y, Tsunoda T, Yamanaka S, Okano H
    • Organizer
      8th IBRO World congress of Neuroscience
    • Place of Presentation
      Florence, Italy
    • Year and Date
      2011-07-16
    • Related Report
      2012 Final Research Report
  • [Presentation] iPS細胞・神経幹細胞で神経系を再生する2011

    • Author(s)
      岡田洋平
    • Organizer
      兵庫県保険医協会第79回総会特別講演
    • Place of Presentation
      神戸
    • Year and Date
      2011-06-19
    • Related Report
      2012 Final Research Report
  • [Presentation] 神経分化と造腫瘍性から迫るヒトiPS細胞の品質評価2011

    • Author(s)
      岡田洋平、岡野栄之
    • Organizer
      第52回日本神経学会学術大会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-05-20
    • Related Report
      2012 Final Research Report
  • [Presentation] Transplantation of human iPS cell-derived neurospheres for the treatment of spinal cord injury in NOD-scid mice2011

    • Author(s)
      Nori, S., Okada Y., Yasuda, A., Tsuji, O., Takahashi,1Y., Kobayashi,Y., Fujiyoshi, K., Toyama, Y., Yamanaka, S., Nakamura, M., Okano, H.
    • Organizer
      ISSCR 9th annual Meeting
    • Place of Presentation
      Toronto, Canada
    • Related Report
      2012 Final Research Report
  • [Presentation] Evaluation of human iPS cells by neural differentiation and tumorigenicity

    • Author(s)
      Okada Y
    • Organizer
      ISSCR 10th annual Meeting
    • Place of Presentation
      Yokohama
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒトiPS細胞由来神経幹細胞の応用とその問題点

    • Author(s)
      岡田洋平
    • Organizer
      第24回日本脳循環代謝学会総会
    • Place of Presentation
      広島
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 不完全にリプログラミングされたヒトiPS細胞由来神経幹細胞は分化誘導に伴うゲノム不安定化によりグリオーマ様腫瘍を形成する

    • Author(s)
      岡田洋平
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] 神経分化と造腫瘍性から迫るヒトiPS細胞の品質評価

    • Author(s)
      岡田洋平、岡野栄之
    • Organizer
      第52回日本神経学会学術大会(招待講演)
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Evaluation of human iPS cells by neural differentiation and tumorigenicity.

    • Author(s)
      Okada Y, Miya F, et al
    • Organizer
      IBRO World congress of Neuroscience
    • Place of Presentation
      フィレンツェ(イタリア)
    • Related Report
      2011 Research-status Report
  • [Presentation] 神経分化と造腫瘍性から迫るヒトiPS細胞の品質評価

    • Author(s)
      岡田洋平、宮冬樹 他
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Book] Brain Nerve2012

    • Author(s)
      海苔聡、辻収彦、岡田洋平、戸山芳昭、岡野栄之、中村雅也
    • Publisher
      医学書院
    • Related Report
      2012 Final Research Report
  • [Book] 臨床神経学2011

    • Author(s)
      岡田洋平、岡野栄之
    • Publisher
      日本神経学会
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.careerpath-prj.keio.ac.jp/kanrinmaru/scholar/okada/index.html

    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] iPS細胞のクローンの選択方法、及びその選択方法に用いる遺伝子の選択方法2012

    • Inventor(s)
      岡田洋平、岡野栄之、角田達彦、宮冬樹
    • Industrial Property Rights Holder
      学校法人慶應義塾
    • Filing Date
      2012-02-27
    • Related Report
      2012 Final Research Report
    • Overseas

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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