Study on the role of extracellular deubiquitinating enzymes
| Project/Area Number |
23650199
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | National Center of Neurology and Psychiatry |
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Principal Investigator |
WADA Keiji 独立行政法人国立精神・神経医療研究センター, 神経研究所疾病研究第四部, 部長 (70250222)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 蛋白質 / 脳神経疾患 / 神経科学 / 生理活性 / 細胞 / 組織 / 脱ユビキチン化酵素 / 神経細胞 / ネットワーク / 生体情報 / 分泌 / パーキンソン病 / モデル動物 / 脳科学 |
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| Research Abstract |
We aimed to elucidate the biological role of extracellular UCH-L1. To achieve this purpose, we used cultured cells and studied whether the protein was secreted or not into the media. We observed that UCH-L1 was secreted into the media via a unconventional pathway. Interestingly, exosome contained UCH-L1. I93M UCH-L1 mutant was less secreted compared with wild type of UCH-L1. Then we studied a possible interaction of UCH-L1 with other disease-related proteins in the secretory event. We found that the secretion of UCH-L1 was modified by the presence of mutated alpha-synuclein and huntingtin. These results suggest that secretion of UCH-L1 into extracellular space might be related to the pathophysiological conditions of neurodegenerative disorders.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Ubiquitin C-terminal hydrolase L1 (UCH-L1) act as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation, independent of its hydrolase activity2013
Author(s)
Kabuta, T., Mitsui, T., Takahashi, M., Fujiwara, Y., Kabuta, C., Konya, C., Tsuchiya, Y., Hatanaka, Y., Uchida, K., Hohjoh, H., Wada, K
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Journal Title
J. Biol. Chem
Volume: 288
Issue: 18
Pages: 12615-12626
DOI
Related Report
Peer Reviewed
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[Journal Article] Sulfatide accumulation in the dystrophic terminals of gracile axonal dystrophy mice: lipid analysis using matrix-assisted laser desorption/ionization imaging mass spectrometry2013
Author(s)
Onishi, K., Tasumi, Y., Wada, K., Yang, H.J., Sugiura, Y., Setou, M., Yoshikawa, H
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Journal Title
Med. Mol. Morphol
Volume: (Epub ahead of print)
NAID
Related Report
Peer Reviewed
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