Functional analysis of neuronal circuit by synaptic photoinactivation

• Project/Area Number: 23650201
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Hokkaido University
• Principal Investigator: KAMIYA Haruyuki 北海道大学, 大学院・医学研究科, 教授 (10194979)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 海馬 / シナプス / グルタミン酸受容体 / 光操作 / 脳・神経 / 神経科学 / 生理学 / 薬理学 / シグナル伝達

Research Abstract

To clarify the roles of individual input in the complex neuronal circuitry, photochemical inactivation of glutamatergic transmission using a photoreactive AMPA receptor antagonist ANQX was adopted for hippocampal slice preparations. Input (layer) specific block was applicable to the hippocampal slice preparation using local UV illumination in combination with application of ANQX.

Research Products

• [Journal Article] Photochemical inactivation analysis of temporal dynamics of postsynaptic native AMPA receptors in hippocampal slices (2012)
  • Author(s): Kamiya, H.
  • Journal Title: Journal of Neuroscience Volume: 32 Issue: 19 Pages: 6517-6524
  • DOI: 10.1523/jneurosci.0720-12.2012
  • NAID: 120004883854
  • Peer Reviewed
• [Journal Article] Heterosynaptic enhancement of the excitability of hippocampal mossy fibers by long-range spill-over of glutamate (2012)
  • Author(s): Uchida T., Fukuda S., Kamiya H.
  • Journal Title: Hippocampus Volume: 22(2) Issue: 2 Pages: 222-229
  • DOI: 10.1002/hipo.20885
  • Peer Reviewed
• [Journal Article] Assessing the roles of presynaptic ryanodine receptors and adenosine receptors in caffeine-induced enhancement of hippocampal mossy fiber transmission (2011)
  • Author(s): Sato I., Kamiya H.
  • Journal Title: Neuroscience Research Volume: 71(3) Issue: 2 Pages: 183-187
  • DOI: 10.1016/j.neures.2011.07.001
  • Peer Reviewed
• [Presentation] AMPA受容体ダイナミクスの光化学的解析 (2013)
  • Author(s): 神谷温之
  • Organizer: 第90回日本生理学会大会(シンポジウム)
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 2013-03-27
• [Presentation] Photoinactivation of AMPA receptors in hippocampal CA1 synapse at physiological temperature (2012)
  • Author(s): Kamiya H.
  • Organizer: Neuroscience 2012.
  • Place of Presentation: Ernest N. Morial Convention Center (USA)
  • Year and Date: 2012-10-13
• [Presentation] 光反応性ブロッカーを用いたグルタミン酸受容体動態のシナプス「その場」解析 (2012)
  • Author(s): 神谷温之
  • Organizer: 第89回日本生理学会大会(シンポジウム)
  • Place of Presentation: 信州大学(長野)
  • Year and Date: 2012-03-31
• [Presentation] 光反応性ブロッカーを用いたグルタミン酸受容体動態のシナプス「その場」解析 (2012)
  • Author(s): 神谷温之
  • Organizer: 第89回日本生理学会(招待講演)
  • Place of Presentation: 信州大学（松本）
• [Presentation] Photoinactivation analysis of postsynaptic AMPA receptor dynamics during hippocampal long-term potentiation (2011)
  • Author(s): Kamiya H.
  • Organizer: Neuroscience 2011.
  • Place of Presentation: Washington ConventionCenter (USA)
  • Year and Date: 2011-11-16
• [Presentation] 単一神経終末エレクトロポレーション法による海馬苔状線維軸索と神経終末の蛍光観察 (2011)
  • Author(s): 神谷温之
  • Organizer: 第34回日本神経科学学会大会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-09-17
• [Presentation] 単一神経終末エレクトロポレーション法による海馬苔状線維軸索と神経終末の蛍光観察 (2011)
  • Author(s): 神谷温之
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: パシフィコ横浜（神奈川）
• [Presentation] Photoinactivation analysis of postsynaptic AMPA receptor dynamics during hippocampal long-term potentiation (2011)
  • Author(s): Kamiya, H.
  • Organizer: Neuroscience 2011
  • Place of Presentation: USA
• [Presentation] Photoinactivation of AMPA receptors in hippocampal CA1 synapse at physiological temperature
  • Author(s): Kamiya, H.
  • Organizer: Neuroscience 2012
  • Place of Presentation: Ernest N. Morial Convention Center (USA)
• [Presentation] AMPA受容体ダイナミクスの光化学的解析
  • Author(s): 神谷温之
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船堀（東京都）
  • Invited
• [Remarks] プレスリリース海馬神経伝達を光でスイッチ・オフ～記憶形成の時間経過を解明～
  • URL: http://www.hokudai.ac.jp/news/120509_pr_med.pdf
• [Remarks] 北海道大学ホームページ プレスリリース （2012/05/09）
  • URL: http://www.hokudai.ac.jp/news/120509_pr_med.pdf