| Project/Area Number |
23650205
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Kyoto University |
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Principal Investigator |
NISHINO Eri 京都大学, 医学(系)研究科(研究院), 助教 (70523992)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | パッチ電極 / オレゴングリーンバプタ / 神経細胞 / 蛍光分光法 / 聴覚機能 / 神経回路 / 蛍光分光 / 蛍光蛋白 / FRET / 電気生理 |
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| Research Abstract |
Photometric patch electrode (PME) recording system is a unique experimental method that enables us a simultaneous recording of electrical activity and optic response of neurons in deep brain tissue. We have applied this PME recording method to auditory system of avian, and found that pattern of Ca response is different depending on the level of integration of auditory information, from a negligible response in the cochlear nucleus, phase locked response in the inferior colliculus to the robust response in the auditory cortex (Field-L). Therefore, this is a challenging, unique and quite powerful new experimental method even international standard. Processing of neuronal information needs continuous plasticity of neural circuits in which Ca ions play pivotal roles; therefore the capability of PME to record both electrical signal and optical Ca signal simultaneously will make PME recording system to play a significant role in the study of neuronal information processing in living animals.
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