| Project/Area Number |
23650208
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Neurophysiology and muscle physiology
|
|---|
| Research Institution | Jikei University School of Medicine |
|---|
Principal Investigator |
KATO Fusao 東京慈恵会医科大学, 医学部, 教授 (20169519)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
WATABE M.ayako 東京慈恵会医科大学, 医学部, 准教授 (00334277)
|
|---|
| Research Collaborator |
TAKAHASHI Yukari 東京慈恵会医科大学, 医学部, 助教 (20613764)
SUGIMURA Yae 東京慈恵会医科大学, 大学院・日本学術振興会, 特別研究員
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 光脳科学 / 神経回路 / 光遺伝学 / 脳 / 扁桃体 / 腕傍核 / シナプス / チャネルロドプシン / シナプス伝達 / 脳スライス / 神経投射 / アデノ随伴ウィルス / feed-forward inhibition / 機能コネクトミクス / 神経科学 / コネクトミクス / 機能的コネクトーム / 逆行性標識 |
|---|
| Research Abstract |
The aim was to develop a method applicable to slice-based analysis of synaptic transmission using optogenetics that can determine the origin of fibers being stimulated. Five to eight weeks after transfecting adeno-associated virus vectors for ChR2 expression into the lateral parabrachial nucleus (LPB) in the dorsal pons of rats, blue light pulses robustly triggered CNQX-sensitive EPSCs in the neurons in the capsular part of the central amygdala recorded in acute slices of distinct angles (coronal and horizontal). These light-evoked EPSCs (leEPSCs) were blocked by TTX but re-appeared by addition of 4-AP, suggesting monosynaptic innervation, and was followed by a large and long-lasting IPSC, indicative of the presence of polysynaptic feed-forward inhibition. This "functional connectomics" technique has advantages in identifying the synaptic properties of long-distance connections, which has been otherwise almost impossible in the conventional electrophysiology setups.
|
