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Screening of voltage-sensitive proteins using Voltage-Sensing Phosphatase, VSP

Research Project

Project/Area Number 23650210
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurophysiology and muscle physiology
Research InstitutionOsaka University

Principal Investigator

OKAMURA Yasushi  大阪大学, 医学系研究科, 教授 (80201987)

Co-Investigator(Kenkyū-buntansha) OKOCHI Yoshifumi  大阪大学, 医学系研究科, 助教 (90435818)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords生理学 / 生体分子 / 蛋白質 / 電位センサー / イオンチャネル / ホスファターゼ / G蛋白共役型受容体 / 神経細胞
Research Abstract

We aimed to develop a functional screening system for novel voltage-sensitive membrane protein modules through making a chimeric protein with voltage-sensing phosphatase. First, we identified Ci-VSP as the most suitable among VSP orthologsfor developing the system, since readout of PI(4,5)P2 phosphatase activity was most remarkable. Ci-VSPwas remodeled into a form in which enzyme activity was more remarkablyenhanced by membrane voltage. Other voltage sensors were fused to the cytoplasmic region of Ci-VSP to acquire chimeric voltage sensing phosphatases.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (81 results)

All 2013 2012 2011 Other

All Journal Article (17 results) (of which Peer Reviewed: 9 results) Presentation (57 results) (of which Invited: 4 results) Book (5 results) Remarks (2 results)

  • [Journal Article] Autoimmune disorder phenotypes in Hvcn1-deficient mice.2013

    • Author(s)
      Sasaki M, Tojo A, Okochi Y, Miyawaki N, Kamimura D, Yamaguchi A, Murakami M, Okamura Y
    • Journal Title

      Biochem.

      Volume: 450 Pages: 295-301

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The cytoplasmic coiled-coil mediates cooperative gating temperature sensitivity in the voltage-gated H+ channel Hv1.2013

    • Author(s)
      Fujiwara Y., Nakagawa A, Okamura Y
    • Journal Title

      SPring-8 Research Frontiers.

      Volume: 0 Pages: 0-0

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Autoimmune disorder phenotypes in HVCN1 gene deficient mice2012

    • Author(s)
      Sasaki M, Tojo A, Okochi Y, Miyawaki N, Kamimura D, Yamaguchi A, Murakami M, Okamura Y
    • Journal Title

      Biochem. J

      Volume: 450(2) Pages: 295-301

    • Related Report
      2012 Final Research Report
  • [Journal Article] Gating of the Designed Trimeric/Tetrameric Voltage-Gated H^<+> Channel2012

    • Author(s)
      Fujiwara Y, Kurokawa T, Takeshita K, Nakagawa A, Larsson HP, Okamura Y
    • Journal Title

      J. Physiol

      Volume: 591 Pages: 627-40

    • Related Report
      2012 Final Research Report
  • [Journal Article] 3' phosphatase activity toward PI(3,4)P2 by voltage-sensing phosphatase, VSP2012

    • Author(s)
      Kurokawa T, Takasuga S, Sakata S, Yamaguchi S, Horie S, Homma KJ, Sasaki T & Okamura Y
    • Journal Title

      Proc. Natl. Acad. Sci. U. S. A

      Volume: 109(25) Pages: 10089-94

    • Related Report
      2012 Final Research Report
  • [Journal Article] The cytoplasmic coiled-coil mediates cooperative gating temperature sensitivity in the voltage-gated H+ channel Hv12012

    • Author(s)
      Fujiwara Y, Kurokawa T, Takeshita K, Kobayashi M, Okochi Y, Nakagawa A & Okamura Y
    • Journal Title

      Nat. Communi.

      Volume: 3 Issue: 1 Pages: 816-816

    • DOI

      10.1038/ncomms1823

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gating of the designed trimeric/tetrameric voltage-gated H+ channel.2012

    • Author(s)
      Fujiwara Y, Kurokawa T, Takeshita K, Nakagawa A, Larsson HP, Okamura Y
    • Journal Title

      J. Physiol.

      Volume: 591 Pages: 627-40

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] KCNQ1 subdomains involved in KCNE modulation revealed by an invertebrate KCNQ1 orthologue2011

    • Author(s)
      Nakajo K, Nishino A, Okamura Y, Kubo Y
    • Journal Title

      J. Gen. Physiol

      Volume: 138(5) Pages: 521-35

    • Related Report
      2012 Final Research Report
  • [Journal Article] Crystal structure of the cytoplasmic PTEN-like region of Ci-VSP provides insight into substrate specificity and redox regulation of the phosphoinositide phosphatase activity2011

    • Author(s)
      Matsuda M, Takeshita K, Kurokawa T, Sakata S, Suzuki M, Yamashita E, Okamura Y, Nakagawa A
    • Journal Title

      J. Biol. Chem

      Volume: 286(26) Pages: 23368-77

    • Related Report
      2012 Final Research Report
  • [Journal Article] Coupling of the phosphatase activity of Ci-VSP to its voltage sensor activity over the entire range of voltage sensitivity2011

    • Author(s)
      Sakata S, Hossain MI, Okamura Y
    • Journal Title

      J. Physiol

      Volume: 589(11) Pages: 2687-705

    • Related Report
      2012 Final Research Report
  • [Journal Article] Coupling of the phosphatase activity of Ci-VSP to its voltage sensor activity over the entire range of voltage sensitivity.2011

    • Author(s)
      Sakata S, Hossain MI, Okamura Y
    • Journal Title

      The Journal of Physiology

      Volume: 589 Issue: 11 Pages: 2687-2705

    • DOI

      10.1113/jphysiol.2011.208165

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the cytoplasmic PTEN-like region of Ci-VSP provides insight into substrate specificity and redox regulation of the phosphoinositide phosphatase activity.2011

    • Author(s)
      Matsuda M, Takeshita K, Kurokawa T, Sakata S, Suzuki M, Yamashita E, Okamura Y, Nakagawa A
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 286 Issue: 26 Pages: 23368-23377

    • DOI

      10.1074/jbc.m110.214361

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Voltage-sensing phosphatase: its molecular relationship with PTEN.2011

    • Author(s)
      OkamuraY, Dixon JE
    • Journal Title

      Physiology

      Volume: 26 Issue: 1 Pages: 6-13

    • DOI

      10.1152/physiol.00035.2010

    • Related Report
      2011 Research-status Report
  • [Journal Article] Gene expression profile of Ci-VSP in juveniles and adult blood cells of ascidian.2011

    • Author(s)
      Ogasawara M, Sasaki M, Nakazawa N, Nishino A, OkamuraY
    • Journal Title

      Gene Expression Pattern

      Volume: 11 Issue: 3-4 Pages: 233-238

    • DOI

      10.1016/j.gep.2010.12.004

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Voltage sensitive phosphoinositide phosphatases of Xenopus: their tissue distribution and voltage dependence.2011

    • Author(s)
      Ratzan WJ, Evsikov AV, Okamura Y, Jaffe LA
    • Journal Title

      Journal of Cellular Physiology

      Volume: 226 Issue: 11 Pages: 2740-2746

    • DOI

      10.1002/jcp.22854

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] KCNQ1 subdomains involved in KCNE modulation revealed by an invertebrate KCNQ1 orthologue.2011

    • Author(s)
      Nakajo K, Nishino A, Okamura Y, Kubo Y
    • Journal Title

      J Gen Physiol.

      Volume: 138 Issue: 5 Pages: 521-535

    • DOI

      10.1085/jgp.201110677

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A self-limiting regulation of TRPC3/C6/C7 channels coupled with PI(4,5)P2-diacylglycerol signalling.2011

    • Author(s)
      Imai Y, Itsuki K, Okamura Y, Inoue R, Mori MX
    • Journal Title

      The Journal of Physiology

      Volume: 590 Issue: 5 Pages: 1101-1119

    • DOI

      10.1113/jphysiol.2011.221358

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Regulatory roles of the dimeric structure in the voltage-gated H+ channel2013

    • Author(s)
      Fujiwara Y, Kurokawa T, Takeshita K, Kobayashi M, Nakagawa A, Okamura Y
    • Organizer
      日本生理学会第90回大会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] How excited to talk with PI?: Lesson from voltage-sensing phosphatase2013

    • Author(s)
      Okamura Y
    • Organizer
      日本生理学会第90回大会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Substrate specificity of the voltage-sensing phosphatase (VSP) changes with membrane potential2013

    • Author(s)
      Kurokawa T, Takasuga S, Sakata S, Yamaguchi S, Shigeo H, Homma K, Sasaki T, Okamura Y
    • Organizer
      日本生理学会第90回大会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Graded tuning of phosphatase activity of VSP coupled with the intermediate state of the voltage sensor2013

    • Author(s)
      Sakata S, Okamura Y
    • Organizer
      Biophys. J
    • Related Report
      2012 Final Research Report
  • [Presentation] Calcium transients in muscle fibers expressing voltage-sensitive phosphoinositide phosphatases2013

    • Author(s)
      Loustau T, Legrand C, Berthier C, Okamura Y, Jacquemond V
    • Organizer
      Biophys. J
    • Related Report
      2012 Final Research Report
  • [Presentation] Physiological genomics of amphibian VSP: a potential molecular mechanism of electrical block of fertilization in vertebrates2013

    • Author(s)
      Mutua J, Jinno Y, Ueno S, Sakata S, Okochi Y, Tsutsui H, Iwao Y, Jaffe L, Okamura Y
    • Organizer
      日本生理学会第90回大会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Regulatory roles of the dimeric structure in the voltage-gated H+ channel.2013

    • Author(s)
      Fujiwara Y, Kurokawa T, Takeshita K, Kobayashi M, Nakagawa A, Okamura Y
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京、タワーホール船堀
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] How excited to talk with PI?: Lesson from voltage-sensing phosphatase.2013

    • Author(s)
      Okamura Y
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京、タワーホール船堀
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Rapid evaluation of optical voltage reporters using field-induced transmembrane potential response.2013

    • Author(s)
      Tsutsui H, Okamura Y
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京、タワーホール船堀
    • Related Report
      2012 Annual Research Report
  • [Presentation] Substrate specificity of the voltage-sensing phosphatase (VSP) changes with membrane potential.2013

    • Author(s)
      Kurokawa T, Takasuga S, Sakata S, Yamaguchi S, Shigeo H, Homma K, Sasaki T, Okamura Y
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京、タワーホール船堀
    • Related Report
      2012 Annual Research Report
  • [Presentation] Graded tuning of phosphatase activity of VSP coupled with the intermediate state of the voltage sensor.2013

    • Author(s)
      Sakata S, Okamura Y
    • Organizer
      Biophysical Society 57th Annual Meeting
    • Place of Presentation
      Philadelphia, PA, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Calcium transients in muscle fibers expressing voltage-sensitive phosphoinositide phosphatases.2013

    • Author(s)
      Loustau T, Legrand C, Berthier C, Okamura Y, Jacquemond V
    • Organizer
      Biophysical Society 57th Annual Meeting
    • Place of Presentation
      Philadelphia, PA, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] PIP2 dynamics underlying muscarinic or vasopressin receptor-activated TRPC3 C6 and C7 currents.2013

    • Author(s)
      Itsuki K, Imai Y, Okamura Y, Inoue R, Mori, MX
    • Organizer
      Biophysical Society 57th Annual Meeting
    • Place of Presentation
      Philadelphia, PA, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Structural bases of the gating regulation in the voltage-gated H+ channel.2013

    • Author(s)
      Fujiwara Y, Kurokawa T, Okamura Y
    • Organizer
      Nagoya Symposium -Frontiers in Structural Physiology
    • Place of Presentation
      愛知県 名古屋市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Physiological genomics of amphibian VSP: a potential molecular mechanism of electrical block of fertilization in vertebrates.2013

    • Author(s)
      Mutua J, Jinno Y, Ueno S, Sakata S, Okochi Y, Tsutsui H, Iwao Y, Jaffe L, Okamura Y
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京、タワーホール船堀
    • Related Report
      2012 Annual Research Report
  • [Presentation] Phosphatase activity of VSP is graded in a reflection of the sequential motion of the voltage sensor.2013

    • Author(s)
      Sakata S, Okamura Y
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京、タワーホール船堀
    • Related Report
      2012 Annual Research Report
  • [Presentation] The optimization of the electrophysiological characterization of the voltage-gated proton channel on the artificial membrane.2013

    • Author(s)
      Kawanabe A, Okamura Y
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京、タワーホール船堀
    • Related Report
      2012 Annual Research Report
  • [Presentation] Autoimmune disorder phenotypes in Hvcn1-deficient mice.2013

    • Author(s)
      Sasaki M, Tojo A, Okochi Y, Miyawaki N, Kamimura D, Yamaguchi A, Murakami M, Okamura Y
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京、タワーホール船堀
    • Related Report
      2012 Annual Research Report
  • [Presentation] Subcellular localization of voltage-gated proton channels in primary cultured microglia.2013

    • Author(s)
      Kawai T, Imura Y, Koizumi S, Okochi Y, Sakimura K, Okamura Y
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京、タワーホール船堀
    • Related Report
      2012 Annual Research Report
  • [Presentation] Secret lives of voltage-sensing phosphatases2012

    • Author(s)
      Okamura Y
    • Organizer
      Gordon Re-search Con-ference "Bio-electro-chemistry"
    • Place of Presentation
      Lucca, Italy
    • Year and Date
      2012-07-03
    • Related Report
      2012 Final Research Report
  • [Presentation] Gordon Research Conference "Protons and Membrane Reactions"2012

    • Author(s)
      Okamura Y
    • Organizer
      Invited talk
    • Place of Presentation
      Ventura, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Phosphatase activity toward phosphatidylinositol 3,4-bisphosphate [PI(3,4)P2] by voltage-sensing phosphatase (VSP)2012

    • Author(s)
      Kurokawa T, Takasuga S, Sakata S, Yamaguchi S, Horie S, Homma KJ, Sasaki T, Okamura Y
    • Organizer
      日本生化学会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] Secret lives of voltage-sensing phosphatases.2012

    • Author(s)
      Okamura Y
    • Organizer
      Gordon Research Conference "Bioelectrochemistry"
    • Place of Presentation
      Lucca, Italy
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Molecular properties of voltage-sensing phosphatases and applications to molecular tools.2012

    • Author(s)
      Okamura Y, Tsutsui H
    • Organizer
      The 1st NTU-Osaka U Joint Symposium on Biomedical Science
    • Place of Presentation
      台北 台湾
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 3' Phosphatase activity toward phosphatidylinositol 3,4-bisphosphate [PI(3,4)P2] by voltage-sensing phosphatase (VSP).2012

    • Author(s)
      Kurokawa T, Takasuga S, Sakata S, Yamaguchi S, Horie S, Homma KJ, Sasaki T, Okamura Y
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡県 福岡市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Cytoplasmic Coiled-Coil Mediates the Gating Coupling and the Temperature Sensitivity in the Voltage-gated H+ Channel2012

    • Author(s)
      藤原祐一郎、黒川竜紀、竹下浩平、小林恵、中川敦史、岡村康司
    • Organizer
      HD Physiology国際シンポジウム
    • Place of Presentation
      東大武田ホール(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 細胞膜たんぱく質による膜電位変化のセンシングと情報転換.2012

    • Author(s)
      岡村康司
    • Organizer
      ナノ・バイオICTシンポジウム2012(招待講演)
    • Place of Presentation
      東京ビッグサイト(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] Gating Mechanisms of Voltage-Gated Proton Channels.2012

    • Author(s)
      岡村康司
    • Organizer
      Gordon Research Conference(招待講演)
    • Place of Presentation
      ベンチュラ(米国)
    • Related Report
      2011 Research-status Report
  • [Presentation] Unusual features of chick vsp and its potential role in regulation of cell structure.2012

    • Author(s)
      Yasushi Okamura, Tatsuki Kurokawa, Shinji Yamaguchi, Naoya Aoki, Ikuko Taira, Souhei Sakata, Koichi Homma
    • Organizer
      第55回生物物理学会大会(米国)
    • Place of Presentation
      サンディエゴ(米国)
    • Related Report
      2011 Research-status Report
  • [Presentation] Ci-KCNQ1, an Ortholog of Vertebrate KCNQ1 from Ciona Intestinalis, has Revealed that KCNE1 and KCNE3 Utilize Different Domains of KCNQ1 for the Modulation of Gating.2012

    • Author(s)
      Koichi Nakajo, Atsuo Nishino, Yasushi Okamura, Yoshihiro Kubo
    • Organizer
      第55回生物物理学会大会(米国)
    • Place of Presentation
      サンディエゴ(米国)
    • Related Report
      2011 Research-status Report
  • [Presentation] A Self-limiting Regulation of TRPC3/C6/C7 channels linked with PI(4,5)P2-Diacylglycerol Signaling.2012

    • Author(s)
      Yuko Imai, Kyohei Itsuki, Yasushi Okamura, Ryuji Inoue, Mori X. Masayuki
    • Organizer
      第55回生物物理学会大会(米国)
    • Place of Presentation
      サンディエゴ(米国)
    • Related Report
      2011 Research-status Report
  • [Presentation] Gating of Trimerized or Tetramerized Voltage-Gated H+ Channels 3476-pos2012

    • Author(s)
      藤原祐一郎、マルタペレス、黒川竜紀、ピータラルソン、岡村康司
    • Organizer
      第55回生物物理学会大会(米国)
    • Place of Presentation
      サンディエゴ(米国)
    • Related Report
      2011 Research-status Report
  • [Presentation] 電位依存性プロトンチャネル(VSOP)の構造と機能2012

    • Author(s)
      岡村康司、藤原祐一郎、大河内善史、筒井秀和、坂田宗平、川鍋陽、中川敦史、竹下浩平、松田真
    • Organizer
      ターゲットタンパク研究プログラムシンポジウム
    • Place of Presentation
      東京国際フォーラム(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 生命における電気信号の秘密を探る2012

    • Author(s)
      岡村康司、藤原祐一郎、大河内善史、坂田宗平、筒井秀和 中川敦史、竹下浩平、松田真
    • Organizer
      ターゲットタンパク研究プログラムシンポジウム
    • Place of Presentation
      東京国際フォーラム(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 電位感受性ホスファターゼ(VSP)の構造と機能2012

    • Author(s)
      岡村康司、藤原祐一郎、大河内善史、筒井秀和、坂田宗平、川鍋陽、中川敦史、竹下浩平、松田真
    • Organizer
      ターゲットタンパク研究プログラムシンポジウム
    • Place of Presentation
      東京国際フォーラム(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 電位センサードメイン蛋白の構造基盤:モジュールの共役による分子機能2012

    • Author(s)
      岡村康司、藤原祐一郎、大河内善史、筒井秀和、坂田宗平、川鍋陽、中川敦史、竹下浩平、松田真
    • Organizer
      ターゲットタンパク研究プログラムシンポジウム(招待講演)
    • Place of Presentation
      東京国際フォーラム(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 3,4 量体型へ人工的にデザインした電位依存性プロトンチャネルのゲーティング2012

    • Author(s)
      藤原祐一郎、黒川竜紀、岡村康司
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本文化会館(長野県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 電位依存性ホスファターゼ(VSP)の電位センサーの変異体における電位依存的酵素活性の計測2012

    • Author(s)
      坂田宗平、ホサインモハマド、岡村康司
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本文化会館(長野県)
    • Related Report
      2011 Research-status Report
  • [Presentation] KCNE3 によるゲーティング修飾におけるKCNQ1 チャネルS1 セグメントの機能的役割2012

    • Author(s)
      中條浩一、西野敦雄、岡村康司、久保義弘
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本文化会館(長野県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 人工膜に再構成した電位依存性プロトンチャネルVSOP のチャネル活性2012

    • Author(s)
      川鍋陽、岡村康司
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本文化会館(長野県)
    • Related Report
      2011 Research-status Report
  • [Presentation] DAG 活性化型TRPC チャネルにおける電流、細胞内Ca2+とPI(4,5)P2 の相関2012

    • Author(s)
      齊郷平、今井裕子、岡村康司、井上隆司、森誠之
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本文化会館(長野県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 感染防御機構における電位依存性プロトンチャネルVSOP の役割の解析2012

    • Author(s)
      大河内善史、荒谷康昭、宮脇奈那、 佐々木真理、鈴木和男、岡村康司
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本文化会館(長野県)
    • Related Report
      2011 Research-status Report
  • [Presentation] VSP による細胞形態制御2012

    • Author(s)
      岡村康司、山口真二、黒川竜紀、坂田宗平、青木直哉、平郁子、本間光一
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本文化会館(長野県)
    • Related Report
      2011 Research-status Report
  • [Presentation] PIP2-PLC-DAGシグナルと連動したTRPC3/C6/C7チャネルの自律的制御機構2012

    • Author(s)
      森誠之、今井裕子、齋郷平、岡村康司、井上隆司
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本文化会館(長野県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 電位依存性プロトンチャネルの2量体会合はチャネルの温度感受性を制御する2011

    • Author(s)
      藤原 祐一郎、黒川竜紀、竹下浩平、小林 恵、中川 敦史、岡村 康司
    • Organizer
      第10回蛋白質科学会大会(招待講演)
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Related Report
      2011 Research-status Report
  • [Presentation] 電位依存性プロトンチャネルVSOPの物理化学的手法を用いた解析2011

    • Author(s)
      Akira Kawanabe, Tetsuya Fukuda, Hideki Kandori, Yasushi Okamura
    • Organizer
      日本生物物理学会
    • Place of Presentation
      姫路(兵庫県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Characterizations of chick voltage-sensing phosphatase.2011

    • Author(s)
      Yasushi Okamura, Tatsuki Kurokawa, Souhei Sakata, Shinji Yamaguchi, Naoya Aoki, Taira Ikuko, Kouichi Homma
    • Organizer
      7th FAOPS Congress
    • Place of Presentation
      台北(台湾)
    • Related Report
      2011 Research-status Report
  • [Presentation] 電位依存性ホスファターゼVSPの作動原理:基質特異性とオルソログ分子の解析2011

    • Author(s)
      黒川竜紀、坂田宗平、岡村康司
    • Organizer
      生理学研究所研究会
    • Place of Presentation
      岡崎(愛知県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Characterizations of chick voltage-sensing phosphatase and its possible role in regulation of cell structure.2011

    • Author(s)
      Yasushi Okamura, Shinji Yamaguchi, Ikuko Taira, Tatsuki Kurokawa, Naoya Aoki, Souhei Sakata & Koichi J. Homma
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] ホヤ幼生の尾の運動制御機構2011

    • Author(s)
      西野敦雄、馬場昭次、岡村康司
    • Organizer
      日本動物学会第82回旭川大会
    • Place of Presentation
      旭川(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] 電位依存性プロトンチャネルVSOP のチャネル活性の定量解析2011

    • Author(s)
      川鍋陽、岡村康司
    • Organizer
      第104回近畿生理学会談話会
    • Place of Presentation
      高槻(大阪府)
    • Related Report
      2011 Research-status Report
  • [Presentation] 感染防御機構における電位依存性プロトンチャネルVSOP の役割の解析2011

    • Author(s)
      大河内善史、荒谷康昭、宮脇奈那、佐々木真理、鈴木和男、岡村康司
    • Organizer
      第104回近畿生理学会談話会
    • Place of Presentation
      高槻(大阪府)
    • Related Report
      2011 Research-status Report
  • [Presentation] Molecular mechanisms of voltage-sensor domain proteins.2011

    • Author(s)
      Yasushi Okamura, Tatsuki Kurokawa, Morten H.H. Norholm, Gunnar von Heijne, Declan Dolye,Jack E. Dixon
    • Organizer
      第34回日本分子生物学会年会(招待講演)
    • Place of Presentation
      横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Molecular mechanisms and potential biological roles of voltage-sensing phosphoinositide phosphatases2011

    • Author(s)
      Yasushi Okamura
    • Organizer
      第10回JBSバイオフロンティア国際シンポジウム(招待講演)
    • Place of Presentation
      福岡(福岡県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Characterization of substrate specificity of voltage-sensing phosphoinositide phosphatase, VSP, with relationship to PTEN2011

    • Author(s)
      Tatsuki Kurokawa
    • Organizer
      第10回JBSバイオフロンティア国際シンポジウム
    • Place of Presentation
      福岡(福岡県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Crystal structure of the cytoplasmic PTEN-like region of Ci-VSP provides insight into substrate specificity and redox regulation of the phosphoinositide phosphatase activity2011

    • Author(s)
      Makoto Matsuda
    • Organizer
      第10回JBSバイオフロンティア国際シンポジウム
    • Place of Presentation
      福岡(福岡県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Coupling of the phosphoinositide phosphatase activity of Ci-VSP to its voltage sensor2011

    • Author(s)
      Souhei Sakata
    • Organizer
      第10回JBSバイオフロンティア国際シンポジウム
    • Place of Presentation
      福岡(福岡県)
    • Related Report
      2011 Research-status Report
  • [Book] 生物学辞典 第五版2013

    • Author(s)
      岡村康司
    • Total Pages
      2192
    • Publisher
      岩波書店
    • Related Report
      2012 Annual Research Report
  • [Book] 脳科学辞典「ナトリウムチャネル」 Brain Science Dictionary (on line)2013

    • Author(s)
      坂田宗平、岡村康司
    • Publisher
      脳科学辞典編集委員会
    • Related Report
      2012 Annual Research Report
  • [Book] 脳科学辞典「膜電位センサー」 Brain Science Dictionary (on line)2013

    • Author(s)
      藤原祐一郎、岡村康司
    • Publisher
      脳科学辞典編集委員会
    • Related Report
      2012 Annual Research Report
  • [Book] Comprehensive Biophysics2013

    • Author(s)
      Okamura Y
    • Publisher
      Academic Press
    • Related Report
      2012 Annual Research Report
  • [Book] Voltage-gated proton channels2012

    • Author(s)
      Okamura Y
    • Publisher
      Oxford: Academic Press
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/phys2/okamura/

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/phys2/okamura/research/present.html

    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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