Comprehensive analysis of in vivo substrates of protein kinase C

• Project/Area Number: 23650214
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Fusional basic brain science
• Research Institution: The University of Tokyo
• Principal Investigator: AIBA Atsu 東京大学, 大学院・医学系研究科, 教授 (20271116)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脳分子プロファイリング / 遺伝子操作マウス / プロテインキナーゼC / プロテオミクス

Research Abstract

Protein kinase C (PKC) γ is involved in cerebellar functions such as motor coordination and elimination of climbing fiber-Purkinje cell synapses. Molecular mechanisms of PKCγ-regulating cerebellar processes are elusive, due to the heretofore undescribed in vivo substrates of PKCγ. In this study, a series of candidate molecules for PKCγ substrates in mouse cerebellum was identifiedby quantitative phospho-proteomic analysis.

Research Products

• [Journal Article] Ras-related C3 botulinum toxin substrate 1 (RAC1) regulates glucose-stimulated insulin secretion via modulation of F-actin (2013)
  • Author(s): Asahara S
  • Journal Title: Diabetologia Volume: なし Issue: 5 Pages: 1088-1097
  • DOI: 10.1007/s00125-013-2849-5
  • Peer Reviewed
• [Journal Article] Guidepost neurons for the lateral olfactory tract : expression of metabotropic glutamate receptor 1 and linnervation by glutamatergic olfactory bulb axons (2012)
  • Author(s): Hirata, T., Kumada, T., Kawasaki, T., Furukawa, T., Aiba, A., Conquet, F., Saga, Y. and Fukuda, A.
  • Journal Title: Dev. Neurobiol Volume: (in press) Issue: 12 Pages: 1559-1576
  • DOI: 10.1002/dneu.22030
  • Peer Reviewed
• [Journal Article] Functional coupling of the metabotropic glutamate receptor, InsP3 receptor and L-type Ca2+ channel in mouseCA1 pyramidal cells. (2012)
  • Author(s): Kato HK, Kassai H, Watabe AM, Aiba A, Manabe T
  • Journal Title: Journal of Physiology Volume: 590 Issue: 13 Pages: 3019-3034
  • DOI: 10.1113/jphysiol.2012.232942
  • Peer Reviewed
• [Journal Article] In vivo imaging visualizes discoid platelet aggregations without endothelium disruption and implicates contribution of inflammatory cytokine and integrin signaling (2012)
  • Author(s): Nishimura S, Manabe I, Nagasaki M, Kakuta S, Iwakura Y, Takayama N, Ooehara J, Otsu M, Kamiya A, Petrich BG, Urano T, Kadono T,Sato S, Aiba A, Yamashita H, Sugiura S, Kadowaki T, Nakauchi H, Eto K, Nagai R
  • Journal Title: Blood Volume: 119
  • Peer Reviewed
• [Journal Article] Cdc42 is required for chondrogenesis and interdigital programmed cell death during limb development (2011)
  • Author(s): Aizawa R, Yamada A, Suzuki D, Iimura T, Kassai H, Harada T, Tsukasaki M, Yamamoto G, Tachikawa T, Nakao K, Yamamoto M, Yamagchi A, Aiba A, Kamijo R
  • Journal Title: AMech Dev Volume: (In press) Issue: 1-4 Pages: 38-50
  • DOI: 10.1016/j.mod.2012.02.002
  • Peer Reviewed
• [Journal Article] Suppression of a neocortical potassium channel activity by intracellular amyloid-ss and its rescue with Homer1a (2011)
  • Author(s): Yamamoto K, Ueta Y, Wang L, Yamamoto R, Inoue N, Inokuchi K, Aiba A, Yonekura H, Kato N
  • Journal Title: Journal of Neuroscience Volume: 31 Pages: 11100-11109
  • Peer Reviewed
• [Journal Article] In vivo imaging visualizes discoid platelet aggregations without endothelium disruption and implicates contribution of inflammatory cytokine and integrin signaling (2011)
  • Author(s): S.Nishimura
  • Journal Title: Blood Volume: 119 Issue: 8 Pages: 128-131
  • DOI: 10.1182/blood-2011-09-381400
  • Peer Reviewed
• [Journal Article] Suppression of a neocortical potassium channel activity by intracellular amyloid-β and its rescue with Homer1a. (2011)
  • Author(s): Yamamoto K, Ueta Y, Wang L, Yamamoto R, Inoue N, Inokuchi K, Aiba A, Yonekura H, Kato N.
  • Journal Title: Journal of Neuroscience Volume: 31 Issue: 31 Pages: 11100-11109
  • DOI: 10.1523/jneurosci.6752-10.2011
  • Peer Reviewed
• [Presentation] 中枢神経系において恒常的活性化型mTORを発現するマウスの作製と解析 (2012)
  • Author(s): 饗場篤
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-13
• [Presentation] 中枢神経系において恒常的活性化型mTORを発現するマウスの作製と解析 (2012)
  • Author(s): 饗場 篤
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] Genetic dissection of mGluR1 function in cerebellar Purkinje cells (2011)
  • Author(s): Aiba A
  • Organizer: 7th International Meeting on Metabotropic Glutamate Receptors
  • Place of Presentation: Grande Albergo Capotaormina (Italy)
  • Year and Date: 2011-10-03
• [Presentation] Genetic dissection of mGluR1 function in cerebellar Purkinje cells (2011)
  • Author(s): Aiba, A
  • Organizer: 7th International Meeting on Metabotropic Glutamate Receptors(招待講演)
  • Place of Presentation: Capotaormina (Italy)
• [Book] 「mTORシグナルによる脳機能の調節と破綻」細胞工学 (2012)
  • Author(s): 葛西秀俊,饗場篤
  • Publisher: 学研メディカル秀潤社
• [Remarks]
  • URL: http://lar.cdbim.m.u-tokyo.ac.jp/
• [Remarks] 東京大学大学院医学系研究科疾患生命工学センター動物資源学部門ホームページ
  • URL: http://lar.cdbim.m.u-tokyo.ac.jp/index.html