| Project/Area Number |
23650216
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Fusional basic brain science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 電位 / 磁気共鳴 / 非侵襲測定 / 神経科学 |
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| Research Abstract |
In this study, we examined cytotoxicity and hazardous dose of pH responsive compounds and found these molecules are low toxic when the concentrations are lower than several mM. We also made a sensitive detecting device which allows a sophisticated analysis of the compounds which has very wide ESR linewidth and low S/N ration.With the use of X-band ESR spectroscopy or Overhauser enhanced MRI, the pH changes between 6 to 9 was analyzed in 0.1 resolutions. We also synthesized five new molecules that are sensitive to pH changes, and physical properties of the compounds were investigated. Based on the results, we are able to measure potential changes both in vitro and in vivo. Since it is necessary to deliver the molecule to region of interest, the effectiveness of the present method can be improved further by advancing the molecular design, such as increasing the tissues and organelles delivery properties.
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