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Promotion of the 4Rs in animal experiments by the development of a production process for polyclonal antibodies using a goldfish

Research Project

Project/Area Number 23650227
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionTohoku University

Principal Investigator

ASANO Ryutaro  東北大学, 大学院・工学研究科, 准教授 (80323103)

Co-Investigator(Kenkyū-buntansha) UMETSU Mitsuo  東北大学, 大学院・工学研究科, 准教授 (70333846)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords実験動物福祉 / 免疫学 / 生体分子 / 蛋白質 / ポリクローナル抗体 / キンギョ / リ ンパ液 / 魚類抗体 / 魚類 / 金魚
Research Abstract

In this study, we tried to the development of a production process for polyclonal antibodies using a particular kind of goldfish, in which lymph can be sustainably and easily collected from its enlarged corneas containing antibodies. We successfully purified polyclonal antibodies in lymph extracted from goldfish and established the detection system for them based on ELISA. Further, we confirmed it by dot blotting that specific antibodies in lymph can be produced by examining adjuvants at the immunization.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (20 results)

All 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 2 results) Presentation (7 results) Book (5 results)

  • [Journal Article] Construction and humanization of a functional bispecific EGFR × CD16 diabody using a refolding system.2012

    • Author(s)
      Ryutaro Asano
    • Journal Title

      FEBS J.

      Volume: 279 Issue: 2 Pages: 223-233

    • DOI

      10.1111/j.1742-4658.2011.08417.x

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 熊谷泉,進化する抗体医薬2012

    • Author(s)
      浅野竜太郎
    • Journal Title

      リウマチ科

      Volume: 47(6) Pages: 700-706

    • Related Report
      2012 Final Research Report
  • [Journal Article] 進化する抗体医薬2012

    • Author(s)
      浅野竜太郎
    • Journal Title

      リウマチ科

      Volume: 47 Pages: 700-706

    • Related Report
      2012 Annual Research Report
  • [Journal Article] In vitro and in vivo antitumor effects of recombinant bispecific antibodies based on humanized anti-EGFR antibody2011

    • Author(s)
      Watanabe Y, Yasui H, et al
    • Journal Title

      Oncol Rep

      Volume: 26(4) Pages: 949-55

    • DOI

      10.3892/or.2011.1382

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] タンパク質工学を駆使した次世代抗体医薬の創製2011

    • Author(s)
      浅野竜太郎,熊谷泉
    • Journal Title

      医工学治療

      Volume: 23(3) Pages: 218-223

    • Related Report
      2012 Final Research Report
  • [Journal Article] 低分子抗体と抗体様スキャフォールドに基づく次世代抗体医薬の開発2011

    • Author(s)
      浅野竜太郎,熊谷泉
    • Journal Title

      BIOINDUSTRY

      Volume: 28(7) Pages: 22-27

    • Related Report
      2012 Final Research Report
  • [Journal Article] タンパク質工学を駆使した次世代抗体医薬の創製2011

    • Author(s)
      浅野竜太郎, 熊谷 泉
    • Journal Title

      医工学治療

      Volume: 23 Pages: 218-223

    • Related Report
      2011 Research-status Report
  • [Journal Article] 低分子抗体と抗体様スキャフォールドに基づく次世代抗体医薬の開発2011

    • Author(s)
      浅野竜太郎, 熊谷 泉
    • Journal Title

      BIO INDUSTRY

      Volume: 28 Pages: 22-27

    • Related Report
      2011 Research-status Report
  • [Presentation] Study on functionalization of anti-EGFR scFv multimers for cancer therapy2012

    • Author(s)
      浅野竜太郎,小山典明,萩原康世,鉞陽介,古本祥三,梅津光央,熊谷泉
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2012-10-26
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] シーエムシー出版2012

    • Author(s)
      浅野竜太郎,熊谷泉
    • Organizer
      次世代医薬開発に向けた抗体工学の最前線
    • Place of Presentation
      137-143
    • Year and Date
      2012-10-05
    • Related Report
      2012 Final Research Report
  • [Presentation] 低分子がん治療二重特異性抗体の機能の向上を目指した変異導入と機能評価2012

    • Author(s)
      細川勝洸,浅野竜太郎,瀧慎太郎,梅津光央,熊谷泉
    • Organizer
      生物工学会若手研究者の集い夏のセミナー2012
    • Place of Presentation
      宮城
    • Year and Date
      2012-06-30
    • Related Report
      2012 Final Research Report
  • [Presentation] 低分子がん治療二重特異性抗体の機能の向上を目指した変異導入と機能評価2012

    • Author(s)
      細川勝洸
    • Organizer
      生物工学会若手研究者の集い 夏のセミナー 2012
    • Place of Presentation
      宮城
    • Related Report
      2012 Annual Research Report
  • [Presentation] Detailed Analysis on an effective multimeric molecule of small recombinant bispecific antibody2012

    • Author(s)
      浅野竜太郎
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] In vivo antitumor effect of anti-EGFR scFvs induced by multimerization2011

    • Author(s)
      浅野竜太郎,古本祥三,熊谷泉
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-10-05
    • Related Report
      2012 Final Research Report
  • [Presentation] 多量体化がもたらす抗EGFR一本鎖抗体のin vivo抗腫瘍効果

    • Author(s)
      浅野竜太郎, 熊谷 泉
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Research-status Report
  • [Book] 次世代医薬開発に向けた抗体工学の最前線2012

    • Author(s)
      浅野竜太郎,熊谷泉
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Final Research Report
  • [Book] 新機能抗体開発ハンドブック2012

    • Author(s)
      浅野竜太郎,熊谷泉
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2012 Final Research Report
  • [Book] 第III編微生物を用いた製造第5章低分子治療抗体の開発.バイオ医薬品製造の効率化と生産基材の開発2012

    • Author(s)
      浅野竜太郎,熊谷泉
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Final Research Report
  • [Book] 次世代医薬開発に向けた抗体工学の最前線2012

    • Author(s)
      浅野竜太郎
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Annual Research Report
  • [Book] 新機能抗体開発ハンドブック2012

    • Author(s)
      浅野竜太郎
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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