Development of rat ES cells carrying rat Nanog reporter gene

• Project/Area Number: 23650229
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: University of Tsukuba
• Principal Investigator: SUGIYAMA Fmihiro 筑波大学, 医学医療系, 准教授 (90226481)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ラット / ES細胞 / Nanog

Research Abstract

To investigate the establishment of germline-competent rat ES cells, BAC transgenic rats carrying Venus gene and puromycin-resistant gene under the control of rat Nanog gene were generated successfully. Furthermore, we showed that theBAC DNA was inserted into a single endogenous Nanog allele in rat ES cells, suggesting the usefulness of BAC homologous recombination in rat ES cells.

Research Products

• [Journal Article] Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination (2013)
  • Author(s): Hasegawa Y, Daitoku Y, Sekiguchi K, Tanimoto Y, Iijima S, Mizuno S, Ema M, Miwa Y, Mekada K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K
  • Journal Title: Exp Anim Volume: 62(in press)
  • NAID: 130003382716
  • Peer Reviewed
• [Journal Article] Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination (2013)
  • Author(s): Hasegawa G
  • Journal Title: Exp Anim Volume: 62
  • Peer Reviewed
• [Presentation] Establishment of germline-competent ES cells from NZB/BINJ mice (2012)
  • Author(s): Iijima S
  • Organizer: International Society for Stem Cell Research
  • Place of Presentation: Pacific Yokohama, Yokohama
  • Year and Date: 2012-06-13
• [Presentation] 内在性ラットNanog遺伝子発現モニター可能なBACベクターの作製 (2012)
  • Author(s): 飯島沙織
  • Organizer: 日本実験動物科学・技術
  • Place of Presentation: 別府コンベンションセンター、別府市
  • Year and Date: 2012-05-24
• [Presentation] Rosa26 遺伝子座へのCAG/EGFP-tdsRed 遺伝子挿入による新規Cre-reporterマウスの開発 (2012)
  • Author(s): 関口渓人
  • Organizer: 日本実験動物科学・技術
  • Place of Presentation: 別府コンベンションセンター、別府市
  • Year and Date: 2012-05-24
• [Presentation] 内在性ラットNanog遺伝子発現をモニター可能なBACベクターの作製 (2012)
  • Author(s): 飯島沙織
  • Organizer: 日本実験動物学会
  • Place of Presentation: 別府
• [Presentation] Establishment of germline-competent ES cells from NZB/BINJ nice (2012)
  • Author(s): Iijima S
  • Organizer: International Society for Stem Cell Research 10th Annual Meeting
  • Place of Presentation: Yokohama