| Project/Area Number |
23650235
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Osaka University |
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Principal Investigator |
HORIE Kyoji 大阪大学, 医学系・研究科, 准教授 (30333446)
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| Project Period (FY) |
2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | 発生工学 / ES細胞 / バーコード / 変異体 |
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| Research Abstract |
We previously developed a method for rapid isolation of a large number of homozygous mutant mouse ES cell lines. However, we realized that phenotypic analysis of each mutant cell line is a labor-intensive process. In the current study, we have constructed a barcode vector library in which each vector contains a unique nucleotide sequence, and labeled each homozygous mutant cell line with different barcode sequence. This allowed us to evaluate the ratio of each mutant cell line among entire population of a pool of mutant cells using each barcode sequence as an index to represent the corresponding mutant cell line, leading to a rapid phenotypic analysis with pooled cells.
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