Study of cell-cell interaction: Cell manipulation method for tissue regeneration

• Project/Area Number: 23650257
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kyoto University
• Principal Investigator: IWATA Hiroo 京都大学, 再生医科学研究所, 教授 (30160120)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ナノバイオロジー・ナノメディスン / 再生医療 / 単鎖DNA / 接着剤 / 細胞接着 / 細胞凝集体 / リン脂質 / Pottsモデル

Research Abstract

We developed a method for the 2-dimensional patterning of cells via hybridization of membrane-tethered single stranded oligonucleotides to complimentary single stranded oligonucleotides bound to optically transparent glass substrates which allowed us to characterize the long term culture of patterned cells. Patterned substrates immersed in FBS-containing media are shown topermit the adsorption of adhesive serum proteins which allowed for the spreading and engagement of natural cytoskeletal adhesion modes in cells initially attached only through DNA hybridization. We show that the coexisting modes of attachment result in competition between membrane-bound tethers and natural cytoskeletal adhesion machinery as cells attempt to migrate away from their initial points of attachment. This competition ends in the escape of cells from their designated patterns and the ‘winning out’ of cytoskeletal migration forces over the affinity of lipid inserts for the cell membrane.

Research Products

• [Journal Article] Inhibition of instant blood-mediated inflammatory responses by co-immobilization of sCR1 and heparin on islets. (2013)
  • Author(s): Luan NM, Iwata H.
  • Journal Title: Biomaterials Volume: online 9 April Issue: 21 Pages: 5019-5024
  • DOI: 10.1016/j.biomaterials.2013.03.041
  • Peer Reviewed
• [Journal Article] Long term culture of cells patterned on glass via membrane-tethered oligonucleotides. (2013)
  • Author(s): Sakurai, K., Hoffecker, I.T., Iwata, H.
  • Journal Title: Biomaterials Volume: 34(2) Issue: 2 Pages: 361-370
  • DOI: 10.1016/j.biomaterials.2012.09.080
  • Peer Reviewed
• [Journal Article] Cells immobilized on patterns printed in DNA by an inkjet printer (2012)
  • Author(s): Sakurai K, Teramura Y, Iwata H
  • Journal Title: Biomaterials Volume: 32(14) Issue: 14 Pages: 3596-3602
  • DOI: 10.1016/j.biomaterials.2011.01.066
  • NAID: 120002970857
  • Peer Reviewed
• [Presentation] Surface-labeling of pancreatic islets with ssDNA-modified magnetic nanoparticles for MR imaging (2012)
  • Author(s): Kitamura, N., Nakai, R., Iwata, H
  • Organizer: 6th International Symposium on Nanomedicine
  • Place of Presentation: くにびきメッセ島根県立産業交流会館(松江市)
  • Year and Date: 2012-11-30
• [Presentation] ランゲルハンス氏島の移植後 MRI 診断を指向した造影剤の開発 (2012)
  • Author(s): 北村成史,岩田博夫
  • Organizer: 第41回医用高分子シンポジウム
  • Place of Presentation: 東京大学先端科学技術研究センター(東京都)
  • Year and Date: 2012-06-25
• [Presentation] 表面修飾酸化鉄ナノ粒子を用いたランゲルハンス氏島の MR 画像化 (2012)
  • Author(s): 北村成史,岩田博夫
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2012-05-29
• [Presentation] 表面修飾酸化鉄ナノ粒子を用いたランゲルハンス氏島のMR画像化． (2012)
  • Author(s): 北村成史，岩田博夫
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] ランゲルハンス氏島の移植後MRI診断を指向した造影剤の開発． (2012)
  • Author(s): 北村成史，岩田博夫
  • Organizer: 第41回医用高分子シンポジウム
  • Place of Presentation: 東京大学先端科学技術研究センター（東京都）
• [Presentation] Surface-labeling of pancreatic islets with ssDNA-modified magnetic nanoparticles for MR imaging. (2012)
  • Author(s): Kitamura, N., Nakai, R., Iwata, H.
  • Organizer: 6th International Symposium on Nanomedicine
  • Place of Presentation: くにびきメッセ島根県立産業交流会館（松江市）
• [Remarks]
  • URL: http://www.frontier.kyoto-u.ac.jp/te03/index-j.htm