Predicting the fate of lymphocytes by the initial Ca response pattern
| Project/Area Number |
23650258
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyoto University |
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Principal Investigator |
MATSUOKA Satoshi 京都大学, 大学院・医学研究科, 特定准教授 (00263096)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Ayako 京都大学, 大学院・医学研究科, 助教 (00378704)
KIM Bongju 京都大学, 大学院・医学研究科, 特定研究員 (80511823)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | リンパ球 / カルシウム / 抗原受容体 / 細胞死 / 細胞増殖 |
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| Research Abstract |
Lymphocyte cell lines (A20 B cells, etc.) and mouse spleen B lymphocytes were used for analyses. These cells were loaded with Ca2+fluorescent indicators (Fluo-4, etc.). Cytoplasmic Ca2+responses to the stimulation of surface antigen receptors were recorded from individual cells using a fluorescence microscope and the data were collected to make a database. Additionally, systems were developed to detect cell death, apoptosis and mitochondrial membrane potential from individual cells. Statistical analysis and correlational analysis were performed using these digitalized data.
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Report
(3 results)
Research Products
(38 results)
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[Journal Article] Basophils are required for the induction of Th2 immunity to haptens and peptide antigens.2013
Author(s)
Otsuka A, Nakajima S, Kubo M, Egawa G, Honda T, Kitoh A, Nomura T, Hanakawa S, Sagita Moniaga C, Kim B, Matsuoka S, Watanabe T, Miyachi Y , Kabashima K.
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Journal Title
Nature Communications
Volume: 4
Issue: 1
Pages: 1739-1739
DOI
Related Report
Peer Reviewed
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[Journal Article] Impairment of Ubiquitin-Proteasome System by E334K cMyBPC Modifies Channel Proteins, Leading to Electrophysiological Dysfunction2011
Author(s)
Bahrudin U, Morikawa K, Takeuchi A, Kurata Y, Miake J, Mizuta E, Adachi K, Higaki K, Yamamoto Y, Shirayoshi Y, Yoshida A, Kato M, Yamamoto K, Nanba E, Morisaki H, Morisaki T, Matsuoka S, Ninomiya H, Hisatome I
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Journal Title
J Mol Biol
Volume: 413(4)
Issue: 4
Pages: 857-78
DOI
Related Report
Peer Reviewed
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