| Project/Area Number |
23650258
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyoto University |
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Principal Investigator |
MATSUOKA Satoshi 京都大学, 大学院・医学研究科, 特定准教授 (00263096)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Ayako 京都大学, 大学院・医学研究科, 助教 (00378704)
KIM Bongju 京都大学, 大学院・医学研究科, 特定研究員 (80511823)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | リンパ球 / カルシウム / 抗原受容体 / 細胞死 / 細胞増殖 |
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| Research Abstract |
Lymphocyte cell lines (A20 B cells, etc.) and mouse spleen B lymphocytes were used for analyses. These cells were loaded with Ca2+fluorescent indicators (Fluo-4, etc.). Cytoplasmic Ca2+responses to the stimulation of surface antigen receptors were recorded from individual cells using a fluorescence microscope and the data were collected to make a database. Additionally, systems were developed to detect cell death, apoptosis and mitochondrial membrane potential from individual cells. Statistical analysis and correlational analysis were performed using these digitalized data.
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