| Project/Area Number |
23650260
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Osaka University |
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Principal Investigator |
ARAKI Tsutomu 大阪大学, 基礎工学研究科, 教授 (50136214)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUSHIMA Shuichiro 大阪大学, 大学院・基礎工学研究科, 助授 (40362644)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 老化 / 蛍光 / コラーゲン / 皮膚 / 分子間架橋 / 象牙質 / 蛍光寿命 / 生体計測 / 歯 |
|---|
| Research Abstract |
Aging is unavoidable phenomenon in human body. In aged persons, their collagen tissue such as the skin is harder than that in young ones. The collagen reacts with blood glucose non-enzymatically, resulting in production of advanced glycosylation endroducts (AGEs). The AGEs bind to collagen and acts as a cross-link between collagen fibrils, and resultant cross-link causes achage of mechanical property of the collagen tissue.
Aim of this research project is to develop a simple and effective method that can sense AGEs optically. For this purpose, we have focused on nanosecond time-resolved fluorescence of AGEs. Observed fluorescence lifetime of collagen tissue informs degree of accumulation of AGEs in the tissue. To cofirm the accumulation of AGEs, we also carried out immuno-staining of AGE. Using these technique we have examined the aging of dentinal collagen.
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