| Project/Area Number |
23650262
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Okayama University |
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Principal Investigator |
MATSUURA Koji 岡山大学, 異分野融合先端研究コア, 助教 (70443223)
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| Research Collaborator |
OKITSU Osamu 三宅医院, 生殖医療科
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ヒト血清アルブミン / 受精卵培養 / ビリルビン / 重金属 / 重金属イオン |
|---|
| Research Abstract |
To evaluate small molecules non-specifically or specifically bound to the human serum albumin (HSA) including commercially available samples and human serum, we evaluated the small molecules before and after purification of HSA from human serum. The signals derived from bilirubin and cholesterol were detected. We found that a purification method using ethanol was necessary in order to remove bilirubin. 2-cell mouse embryo developed to blastocyst when purified HSA was added to the medium. Furthermore, when the mouse embryo is cultured in culture medium supplemented with fluorescence labeled bovine serum albumin (BSA), fluorescence of the BSA was observed in mouse blastocyst. Based on these results, we considered that fertilized oocyte could uptake the HSA in mouse embryonic development. Even if organic molecules bound to HSA, mouse embryonic development was observed. Because removal of binding molecules such as bilirubin and cholesterol is difficult without addition of salt and ethanol that affect the human embryo culture, we concluded that the HSA purified from human serum was not able to use for the embryo culture.
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