Development of antibody recognition imaging method for cancer diagnosis

• Project/Area Number: 23650271
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Keio University
• Principal Investigator: YANAGAWA Hiroshi 慶應義塾大学, 理工学部, 訪問教授 (40327672)
• Co-Investigator(Kenkyū-buntansha): TABATA Noriko 慶應義塾大学, 理工学研究科, 特任准教授 (60260080)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: iPS細胞 / がん幹細胞 / in vitro virus法 / CD44 / in vivo imaging / CD138

Research Abstract

Since CD44 is considered to be one of the factors required in order to maintain the autonomous replication ability of cancer stem cells as adhesion molecules, we performed to select single strand antibodies which prevent the induced cancer stem

Research Products

• [Journal Article] A phthalimide derivative that inhibits centrosomal clustering is effective on multiple myeloma (2012)
  • Author(s): Shiheido, H., Terada, F., Tabata, N., Hayakawa, I., Matsumura, N., Takashima, H., Ogawa, Y., Du,W., Yamada, T., Shoji, M., Sugai, T., Doi, N.,Iijima, S., Hattori, Y., Yanagawa, H.
  • Journal Title: PLoS ONE Volume: 7 Issue: 6 Pages: e38878-e38878
  • DOI: 10.1371/journal.pone.0038878
  • Peer Reviewed
• [Journal Article] In vitro selection of Fab fragments by mRNA display and gene-linking emulsion PCR (2012)
  • Author(s): Sumida, T., Yanagawa, H., Doi, N.
  • Journal Title: J. Nucleic Acids Volume: 2012 Pages: 371379-371379
  • DOI: 10.1155/2012/371379
  • Peer Reviewed
• [Journal Article] DNA display selection of peptide ligands for a full-length human G protein-coupled receptor on CHO-K1 cells (2011)
  • Author(s): Doi,N.,Yamakawa,N.,Matsumoto,H.,Yamamoto,Y.,Nagano,T.,Matsumura,N.,Horisawa,K.,Yanagawa,H.
  • Journal Title: PLoS ONE Volume: 7 Issue: 1 Pages: 1-8
  • DOI: 10.1371/journal.pone.0030084
  • Peer Reviewed
• [Presentation] In vitroevolution of high-affinity anti-CCR5 single chain Fv via neutral drift libraries (2011)
  • Author(s): Sato, R., Doi,N., Misumi,S., Shoji,S., Matsushima, S., Yokochi, S., Yanagawa, H.
  • Organizer: 34th MBSJ 2011
  • Place of Presentation: Yokohama
  • Year and Date: 2011-12-13
• [Presentation] In vitroselection of anti-CD44v scFv inhibiting CD44v-xCT interaction (2011)
  • Author(s): Umezawa,H., Tabata,N., Sakuma,Y., Takenaka,Y., Matsumoto,Y., Doi,N., Nagano, O., Saya,H., Yanagawa, H.
  • Organizer: 34th MBSJ 2011
  • Place of Presentation: Yokohama
  • Year and Date: 2011-12-13
• [Presentation] In vitroselection of Fc receptor-binding peptides using in vitro virus method (2011)
  • Author(s): Mihara, M., Sumida, T., Sakuma, Y., Tabata, N., Horisawa, K., Yanagawa, H., Doi, N.
  • Organizer: 34th MBSJ 2011
  • Place of Presentation: Yokohama
  • Year and Date: 2011-12-11
• [Presentation] Selection of scFv inhibiting adhesion of induced cancer stem cells by in vitro virus (IVV) method (2011)
  • Author(s): Tabata, N., Sakuma, Y., Shimizu, T., Saya, H., Doi, N., Yanagawa, H.
  • Organizer: 34th MBSJ 2011
  • Place of Presentation: Yokohama
  • Year and Date: 2011-12-11
• [Presentation] In vitro evolution of high-affinity anti-CCR5 single chain Fv via neutral drift libraries (2011)
  • Author(s): 2. Ryota Sato，Nobuhide Doi，Shogo Misumi，Shozo Shoji，Koji Matsushima，Shoji Yokochi，and Hiroshi Yanagawa
  • Organizer: 34th MBSJ 2011
  • Place of Presentation: Yokohama
• [Presentation] In vitro selection of anti-CD44v scFv inhibiting CD44v-xCT interaction (2011)
  • Author(s): 1. Hiroki Umezawa，Noriko Tabata，Yuko Sakuma，Yuma Takenaka，Yoshinori Matsumoto，Nobuhide Doi，Osamu Nagano，Hideyuki Saya，and Hiroshi Yanagawa
  • Organizer: 34th MBSJ 2011
  • Place of Presentation: Yokohama
• [Presentation] In vitro selection of Fc receptor-binding peptides using in vitro virus method
  • Author(s): Mihara M, Sumida T, Sakuma Y, Tabata N, Horisawa K, Yanagawa H, Doi N
  • Organizer: 35th MBSJ 2012
  • Place of Presentation: Fukuoka
• [Presentation] Selection of scFv inhibiting adhesion of induced cancer stem cells by in vitro virus (IVV) method
  • Author(s): Tabata N, Sakuma Y, Shimizu T, Saya H, Doi N, Yanagawa H
  • Organizer: 35th MBSJ 2012
  • Place of Presentation: Fukuoka
• [Book] Chapter 4. Applications of the In Vitro Virus (IVV) Method for Various Protein Functional Analyses, IN:Protein Engineering-Technology and Application, ed. T. Ogawa, pp.85-110 (2012)
  • Author(s): Tabata N, Horisawa K, Yanagawa H
  • Total Pages: 187
  • Publisher: InTech, Rijeka, Croatia
• [Book] Chemical Synthetic Biology (2011)
  • Author(s): Doi, N., Kakukawa, K., Oishi, Y., Yanagawa, H. John Wiley & Sons
  • Publisher: Ltd.
• [Book] Neural Stem Cells and Therapy (2011)
  • Author(s): Horisawa,K., Yanagawa, H
  • Publisher: InTech
• [Book] Chemical Synthetic Biology (2011)
  • Author(s): Nobuhide Doi, Koichi Kakukawa, Yo Oishi, and Hiroshi Yanagawa
  • Total Pages: 8
  • Publisher: High solubility of random-sequence proteins consisting of five kinds of primitive amino acids
• [Book] Drug Delivery System (2011)
  • Author(s): Hiroshi Yanagawa
  • Total Pages: 13
  • Publisher: Development of IVV method into drug discovery
• [Book] Neural Stem Cells and Therapy (2011)
  • Author(s): Kenichi Horisawa and Hiroshi Yanagawa
  • Total Pages: 18
  • Publisher: The Musashi proteins in neural stem cell/progenitor cells