Biomolecular analysis on disturbance of glomerular filtration at the early stage of diabetes
| Project/Area Number |
23650278
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YADA Toyotaka 川崎医科大学, 医学部, 講師 (00210279)
小笠原 康夫 川崎医科大学, 医学部, 准教授 (10152365)
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| Co-Investigator(Renkei-kenkyūsha) |
OGASAWARA Yasuo 川崎医科大学, 医学部, 准教授 (10152365)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 糖尿病 / 糸球体濾過 / C-peptide, ポドサイト / スリット膜蛋白 / ネフリン / ポ ドシン / CD2AP / C-peptide / ポドサイト / ポドシン / スリットダイアフラム / 濾過 |
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| Research Abstract |
We have visualised the early augmented glomerular filtration in-vivo that may be related to the development of diabetic nephropathy with albuminuria by multi-photon microscopy. We hypothesised that there are structural and component protein distributional changes, which may be associated with albuminuria.We used control rats and diabetic Wistar rats. After kidneys were extracted, slit membrane component proteins. Stained images of each component were binarised and the distributions of slit membrane proteins were analysed by area ratio. Area ratio was found decreasing linearly with diabetic duration. Amongst pair combinations of 3 proteins, the best correlation was between the diabetic duration and the area ratio of nephrin and podocin (r=0.871). This means discrepancy of slit membrane protein distribution deteriorates progressively.
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Report
(3 results)
Research Products
(16 results)
