| Project/Area Number |
23650280
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SAKO Yasushi 独立行政法人理化学研究所, 佐甲細胞情報研究室, 主任研究員 (20215700)
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| Co-Investigator(Renkei-kenkyūsha) |
MORITA Shinichi 独立行政法人理化学研究所, 佐甲細胞情報研究室, 協力研究員 (40462741)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 1細胞計測 / 多成分解析 / 分光 / 細胞状態 / 細胞分化 / ラマン分光 / 顕微計測 |
|---|
| Research Abstract |
A human breast cancer cell line, MCF-7 differentiates into mammalian grand-like cells upon addition of heregulin to the culture medium. In this study, we traced changes of cellular internal states by means of Raman spectrum measurement, which is a potent method to detect multi-dimensional intracellular dynamics in single cells with minimal disruption. Analysis of the Raman dynamics up to 12 days though the differentiation process has revealed pseud-circular trajectory of the major intracellular components.
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