Raman spectrum analysis of cell differentiation dynamics

• Project/Area Number: 23650280
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biological material science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SAKO Yasushi 独立行政法人理化学研究所, 佐甲細胞情報研究室, 主任研究員 (20215700)
• Co-Investigator(Renkei-kenkyūsha): MORITA Shinichi 独立行政法人理化学研究所, 佐甲細胞情報研究室, 協力研究員 (40462741)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 1細胞計測 / 多成分解析 / 分光 / 細胞状態 / 細胞分化 / ラマン分光 / 顕微計測

Research Abstract

A human breast cancer cell line, MCF-7 differentiates into mammalian grand-like cells upon addition of heregulin to the culture medium. In this study, we traced changes of cellular internal states by means of Raman spectrum measurement, which is a potent method to detect multi-dimensional intracellular dynamics in single cells with minimal disruption. Analysis of the Raman dynamics up to 12 days though the differentiation process has revealed pseud-circular trajectory of the major intracellular components.

Research Products

• [Journal Article] Raman imaging of the diverse states of the filamentous cyanobacteria. (2013)
  • Author(s): Ishihara, J.
  • Journal Title: Proc. SPIE Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Raman spectroscopic analysis of H2O2-stimulated three-dimensional human skin models. (2013)
  • Author(s): Morita, S.-i.
  • Journal Title: J. Spectrosopy. Volume: 2013 Pages: 9034501-6
  • Peer Reviewed
• [Journal Article] Raman spectroscopic analysis of H2O2-stimulated three-dimensional human skin models (2012)
  • Author(s): Morita, S.-i., Takanezawa, S., Date, A., Watanabe, S., Murakami, T. N., Kawashima, N., Sato, H., and Sako, Y
  • Journal Title: J. Spectrosopy 2013 Volume: 903450 Pages: 6-6
  • Peer Reviewed
• [Journal Article] Single-molecule kinetic analysis of RAF activation in the ternary complex among RAF, RasGTP, and the kinases on the plasma membrane of living cells. (2011)
  • Author(s): Hibino, K., Shibata, T., Yanagida, T., and Sako, Y.
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 36460-36468
• [Journal Article] Live cell single-molecule detection in systems biology (2011)
  • Author(s): Sako, Y, Hiroshima, M., Pack, C.-G., Okamoto, K., Hibino, K., Yamamoto, A.
  • Journal Title: WIREs Systems Biology and Medicine Volume: 4 Issue: 2 Pages: 183-192
  • DOI: 10.1002/wsbm.161
  • Peer Reviewed
• [Journal Article] Raman imaging of the diverse states of the filamentous cyanobacteria
  • Author(s): Ishihara, J., Tachikawa, M., Mochizuki, A., Sako, Y., Iwasaki, H., and Morita, S
  • Journal Title: Proc. SPIE Volume: (in press)
  • Peer Reviewed
• [Presentation] Measurements of single cell dynamics upon stimulation with a differentiation factor using Raman micro-spectroscopy (2012)
  • Author(s): Takanezawa, S., Morita, S.-i., Ozaki, Y., and Sako, Y
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-22
• [Presentation] ヒト皮膚表皮における振動分光法によるシミの超早期発見の検討(2) 新規可視化法の開発 (2012)
  • Author(s): 伊達朗、盛田伸一、遠山和美、佐甲靖志
  • Organizer: 日本薬学会第132年会(ハイライト講演)
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-29
• [Presentation] 細胞運命決定のゆらぎ (2012)
  • Author(s): 佐甲靖志
  • Organizer: 自然科学研究機構プロジェクト合同シンポジウム「脳神経情報の階層的構造」「機能生命科学における揺らぎと決定」
  • Place of Presentation: 岡崎
  • Year and Date: 2012-02-15
• [Presentation] 細胞分化ダイナミクスのラマン計測と相関分光 (2012)
  • Author(s): 高根沢聡太、盛田伸一、佐甲靖志、尾崎幸洋
  • Organizer: 第72回分析化学討論会
  • Place of Presentation: 鹿児島
• [Presentation] ラマン分光法による細胞分化の識別 (2012)
  • Author(s): 高根沢聡太、盛田伸一、廣島通夫、三井敏之、尾崎幸洋、佐甲靖志
  • Organizer: 2012年春季 第59回 応用物理学関係連合講演会
  • Place of Presentation: 東京
• [Presentation] ヒト皮膚色素化のラマン計測 (2012)
  • Author(s): 盛田伸一、高根沢聡太、伊達朗、尾崎幸洋、佐甲靖志
  • Organizer: 2012年春季 第59回 応用物理学関係連合講演会
  • Place of Presentation: 東京
• [Presentation] A phase diagram of cell differentiation based on Raman spectroscopic measurements (2011)
  • Author(s): Morita, S.-i., Takanezawa, S., Hiroshima, M., Mitsui, T., and Sako, Y
  • Organizer: The 49th Annual Meeting of the Biophysical Society
  • Place of Presentation: Japan, Himeji
  • Year and Date: 2011-09-16
• [Presentation] 細胞内反応とそのゆらぎ (2011)
  • Author(s): 佐甲靖志
  • Organizer: 光エレクトロニクス第130委員会 創立50周年記念シンポジウム「光による新しい科学技術・産業の創成」
  • Place of Presentation: 東京
  • Year and Date: 2011-05-13
• [Presentation] A phase diagram of cell differentiation based on Raman spectroscopic measurements. (2011)
  • Author(s): Morita, S.-i., Takanezawa, S., Hiroshima, M., Mitsui, T., and Sako, Y.
  • Organizer: The 49th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Himeji
• [Presentation] Measurements of single cell dynamics upon stimulation with a differentiation factor using Raman micro-spectroscopy.
  • Author(s): Takanezawa, S.
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋
• [Presentation] 細胞分化ダイナミクスのラマン計測と相関分光
  • Author(s): 高根沢聡太
  • Organizer: 第72回分析化学討論会
  • Place of Presentation: 鹿児島
• [Book] Static and dynamic disorder in in vitro reconstituted receptor/adaptor interaction. (2011)
  • Author(s): Takagi, H., Morimatsu, M., and Sako, Y.
  • Publisher: Wiely
• [Remarks]
  • URL: http://www.riken.go.jp/cell-info/
• [Remarks]
  • URL: http://www.riken.go.jp/research/labs/chief/cell_inf/