Super cell-specific gene delivery system using triple securities
| Project/Area Number |
23650290
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 遺伝子送達 / DDS / 遺伝子治療 / がん / プロテインキナーゼ / ナノ粒子 / ナノメディスン |
|---|
| Research Abstract |
In this research, we attempted to improve cell specificity of gene delivery system with multiple securities. Thus, cationic polyethyleneimine and hydrophobic chain were introduced into our previously developed intracellular signal-responsive gene carrier. The carrier improved cancer cell specificity dramatically. Then, PEG chains were also incorporated into the carrier with pH-labile manner to stabilize the polyplex with DNA in blood circulation. Obtained carrier realized 1300 times activation in cancer cell against normal cell condition.
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Report
(3 results)
Research Products
(87 results)
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[Journal Article] A simple set-and-mix assay for screening of protein kinase inhibitors in cell lysates2011
Author(s)
Asami, Y., Oishi, J., Kitazaki, H., Kamimoto, J., Kang, J.-H., Niidome, T., Mori, T., Katayama, Y.
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Journal Title
Analytical Biochemistry
Volume: 418
Issue: 1
Pages: 44-49
DOI
Related Report
Peer Reviewed
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[Journal Article] Fluorescent nanoparticles consisting of lipopeptides and fluorescein-modified polyanions for monitoring of protein kinase activity2011
Author(s)
Koga, H., Toita, R., Mori, T., Tomiyama, T., Kang, J.-H., Niidome, T., Katayama, Y.
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Journal Title
Bioconjugate Chemistry
Volume: 22
Issue: 8
Pages: 1526-1534
DOI
Related Report
Peer Reviewed
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[Journal Article] A hydrophilic polymer grafted with a histone tail peptide as an artificial gene regulator2011
Author(s)
Shiosaki, S., Kuramoto, M., Toita, R., Mori, T.,Niidome, T., Katayama, Y.
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Journal Title
Bioorganic and Medicinal Chemistry
Volume: 19
Issue: 13
Pages: 4101-4105
DOI
Related Report
Peer Reviewed
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[Journal Article] Controlled-release system of single-stranded DNA triggered by the photothermal effect of gold nanorods and its in vivo application2011
Author(s)
Yamashita, S., Fukushima, H., Akiyama, Y., Niidome, Y., Mori, T., Katayama, Y., Niidome, T.
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Journal Title
Bioorganic and Medicinal Chemistry
Volume: 19
Issue: 7
Pages: 2130-2135
DOI
Related Report
Peer Reviewed
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[Journal Article] A solid-in-oil dispersion of gold nanorods can enhance transdermal protein delivery and skin vaccination2011
Author(s)
Pissuwan, D., Nose, K., Kurihara, R., Kaneko, K., Tahara, Y., Kamiya, N., Goto, M., Katayama, Y., Niidome, T.
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Journal Title
Small
Volume: 7
Issue: 2
Pages: 215-220
DOI
Related Report
Peer Reviewed
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[Journal Article] Optimization of Peptide Density on Microarray Surface for Quantitative Phosphoproteomics2011
Author(s)
Shimomura, T., Han, X., Hata, A., Niidome, T., Mori, T., Katayama, Y.
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Journal Title
Analytical Sciences
Volume: 27
Issue: 1
Pages: 13-17
DOI
NAID
ISSN
0910-6340, 1348-2246
Related Report
Peer Reviewed
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[Journal Article] Cancer-specific gene carriers responding to cancer microenvironment: acidosis and hyper-activated protein kinases
Author(s)
S. Kushio, A. Tsuchiya, Y. Nakamura, T. Nobori, C. W. Kim, G. Zhao, D. Funamoto, E. K. Lee, K. Lee, T. Niidome, T. Mor, Y. Katayama
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Journal Title
Biomed. Eng
Volume: (in press)
Related Report
Peer Reviewed
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