One-pot preparation of dendrimer-gold nanohybrids with controlled photothermal property and their application to cancer therapy

• Project/Area Number: 23650293
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Osaka Prefecture University
• Principal Investigator: KONO Kenji 大阪府立大学, 大学院・工学研究科, 教授 (90215187)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ナノバイオ / デンドリマー / 金ナノ粒子 / 癌治療

Research Abstract

In this study, we attempted to develop dendrimer-gold nanorod hybrids with controlled size and photothermal property. The hybrids with small size and high photothermal property were prepared by formation of gold nanorod in the presence of polyethyelen glycol-modified polyamidoamine dendrimers with disulfide bond at the core. Cancer cells taking up the hybrids were killed effectively under near infrared light irradiation and hence these hybrids might be potentially useful as a nanodevice for photothermal therapy for cancer.

Research Products

• [Journal Article] PAMAM dendrimers with oxyethylene unit-enriched surface as biocompatible temperature-sensitive dendrimers (2013)
  • Author(s): X. Li, Y. Haba, K. Ochi, E. Yuba, A. Harada, K. Kono
  • Journal Title: Bioconjugate Chemistry Volume: 24 Pages: 282-290
  • Peer Reviewed
• [Journal Article] PAMAM dendrimers with oxyethylene unit-enriched surface as biocompatible temperature-sensitive dendrimers (2013)
  • Author(s): X. Li, Y. Haba, K. Ochi, E. Yuba, A. Harada, K. Kono
  • Journal Title: Bioconjugate Chem. Volume: 24 Issue: 2 Pages: 282-290
  • DOI: 10.1021/bc300190v
  • Peer Reviewed
• [Journal Article] Assemblies of polyamidoamine dendron-bearing lipids with various generations: their morphologies and abilities of gene transfection (2012)
  • Author(s): S. Iwashita, Y. Hiramatsu, T. Otani, C. Amano, M. Hirai, H. Minematsu, H. Kitagawa, K. Oie, E. Yuba, K. Kono, M. Miyamoto, K. Igarashi
  • Journal Title: J. Biomaterials Applications Volume: 27 Pages: 445-456
  • Peer Reviewed
• [Journal Article] Dendrimer-based bionanomaterials produced by surface modification, assembly, and hybrid formation (2012)
  • Author(s): K. Kono
  • Journal Title: Polymer Journal Volume: 44 Pages: 531-540
  • NAID: 10030598956
  • Peer Reviewed
• [Journal Article] Polyamidoamine dendron-bearing lipids as a non-viral vector: influence of dendron generation (2012)
  • Author(s): K. Kono, R. Ikeda, K. Tsukamoto, E. Yuba, C. Kojima, A. Harada
  • Journal Title: Bioconjugate Chemistry Volume: 23 Pages: 871-879
  • Peer Reviewed
• [Journal Article] Effect of unsaturated alkyl chains on transfection activity of poly(amidoamine)dendron-bearing lipids (2012)
  • Author(s): E. Yuba, Y. Nakajima, K. Tsukamoto, S. Iwashita, C. Kojima, A. Harada, K. Kono
  • Journal Title: J. Control. Release Volume: 160 Pages: 552-560
  • Peer Reviewed
• [Journal Article] Thermosensitive molecular assemblies from PAMAM dendron-based lipids (2011)
  • Author(s): K.Kono, E.Murakami, Y.Hiranaka, E.Yuba, C.Kojima, A.Harada, K.Sakurai
  • Journal Title: Angew.Chem.Int.Ed. Volume: 50 Issue: 28 Pages: 6332-6336
  • DOI: 10.1002/anie.201101007
  • Peer Reviewed
• [Journal Article] Design of a novel drug carrier with photo-responsive properties: drug-encapsulated and alkanethiol-modified gold nanoparticle-loaded PEGylated dendrimer
  • Author(s): C. Kojima, H. Kawabata, A. Harada, H. Horinaka, K. Kono
  • Journal Title: Chem. Lett Volume: (in press)
  • NAID: 10031176025
  • Peer Reviewed
• [Presentation] Dually Functionalized Dendrimers by Temperature-Sensitive Surface Modification and Gold Nanoparticles Loading for Photothermal Therapy (2013)
  • Author(s): K. Kono, K. Takeda, X. Li, E. Yuba and A. Harada
  • Organizer: 2nd International Conference on Biomaterials Science in Tsukuba
  • Place of Presentation: つくば
  • Year and Date: 2013-03-21
• [Presentation] Dually Functionalized Dendrimers by Temperature-Sensitive Surface Modification and gold nanoparticles loading for photothermal therapy (2013)
  • Author(s): K. Kono, K. Takeda, X. Li, E. Yuba, and A. Harada
  • Organizer: ICBS2013
  • Place of Presentation: Tsukuba, Japan
• [Presentation] Preparation of poly(ethylene glycol)-modified PAMAM dendrimers-gold nanorod conjugates and their photothermal properties (2012)
  • Author(s): X. Li, E. Yuba, A. Harada, K. Kono
  • Organizer: 9th SPSJ International Polymer Conference
  • Place of Presentation: 神戸
  • Year and Date: 2012-12-14
• [Presentation] PAMAM デンドリマーと金ナノロッドとのハイブリッド形成 (2012)
  • Author(s): 是枝李香, 李小杰, 武田啓志, 弓場英司, 原田敦史, 河野健司
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-11
• [Presentation] デンドリマーと金ナノ粒子の複合化による光熱治療のための機能性ナノハイブリッドの構築 (2012)
  • Author(s): 河野健司, 李小杰, 是枝李香, 武田啓志, 弓場英司, 原田敦史
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-11
• [Presentation] Biocompatible temperature-sensitive dendrimers encapsulating gold nanoparticles for photothermal therapy (2012)
  • Author(s): X. Li, K. Takeda, E. Yuba, A. Harada, K. Kono
  • Organizer: IUPAC 8th International Conference on Novel Materials and Synthesis
  • Place of Presentation: America
  • Year and Date: 2012-06-26
• [Presentation] Stimuli-sensitive liposomes for drug delivery (2012)
  • Author(s): Kenji Kono
  • Organizer: CIMTEC2012
  • Place of Presentation: Italy
  • Year and Date: 2012-06-14
• [Presentation] オリゴエチレングリコール修飾PAMAM デンドロン-金ナノロッドコンジュゲートの作製と光熱特性 (2012)
  • Author(s): 李小杰, 武田啓志, 弓場英司, 原田敦史, 河野健司
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: 横浜
  • Year and Date: 2012-05-30
• [Presentation] オリゴエチレングリコール修飾PAMAM デンドロン－金ナノロッドコンジュゲートの作製と光熱特性 (2012)
  • Author(s): 李小杰, 武田啓志, 弓場英司, 原田敦史, 河野健司
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: 横浜
• [Presentation] Biocompatible Temperature-sensitive Dendrimers Encapsulating Gold Nanoparticles for Photothermal Therapy (2012)
  • Author(s): X. Li, K. Takeda, E. Yuba, A. Harada and K. Kono
  • Organizer: IUPAC World Polymer Congress
  • Place of Presentation: Blacksburg, USA
• [Presentation] デンドリマーと金ナノ粒子の複合化による光熱治療のための機能性ナノハイブリッドの構築 (2012)
  • Author(s): 河野健司, 李小杰, 是枝李香, 武田啓志, 弓場英司, 原田敦史
  • Organizer: 第61回高分子討論会
  • Place of Presentation: 名古屋
• [Presentation] PAMAM デンドリマーと金ナノロッドとのハイブリッド形成 (2012)
  • Author(s): 是枝李香, 李小杰, 武田啓志, 弓場英司, 原田敦史,河野健司
  • Organizer: 第61回高分子討論会
  • Place of Presentation: 名古屋
• [Presentation] Preparation of Poly(ethylene glycol)-modified PAMAM Dendrimers-gold Nanorod Conjugates and Their Photothermal Properties (2012)
  • Author(s): X. Li, E. Yuba, A. Harada and K. Kono
  • Organizer: The 9th SPSJ International Polymer Conference
  • Place of Presentation: Kobe, Japan
• [Presentation] Preparation of Stimuli-Responsive Dendrimer-Gold Nanoparticle Hybdirs for Biomedical Applications (2011)
  • Author(s): K. Kono, K. Takeda, E. Yuba, A. Harada
  • Organizer: The 12th Pacific Polymer Conference
  • Place of Presentation: Korea
  • Year and Date: 2011-11-15
• [Presentation] Design of Stimuli-sensitive Dendrimers, Dendrimer-gold Hybrids and Dendrimer-based Assemblies for Biomedical Applications (2011)
  • Author(s): K. Kono
  • Organizer: The 7th International Dendrimer Symposium
  • Place of Presentation: America
  • Year and Date: 2011-06-28
• [Presentation] 刺激応答性ナノキャリアの開発 (2011)
  • Author(s): 河野健司
  • Organizer: 第60回高分子学会年次大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-05-27
• [Presentation] 金ナノロッドコア PEG 修飾 PAMAM デンドリマーの作製と光発熱特性 (2011)
  • Author(s): 武田啓志, 弓場英司, 原田敦史, 河野健司
  • Organizer: 第60回高分子学会年次大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-05-27
• [Presentation] 金ナノロッドコアPEG修飾PAMAMデンドリマヸの作製と光発熱特性 (2011)
  • Author(s): 武田、弓場、原田、河野
  • Organizer: 第60回高分子学会年次大会
  • Place of Presentation: 大阪
• [Presentation] Design of Stimuli-sensitive Dendrimers, (2011)
  • Author(s): Kenji Kono
  • Organizer: 7th International Dendrimer Symposium(招待講演)
  • Place of Presentation: Washington DC
• [Presentation] 金ナノロッドコアPEG 修飾PAMAM デンドリマーの光熱特性制御 (2011)
  • Author(s): 武田、弓場、原田、河野
  • Organizer: 第60回高分子討論会
  • Place of Presentation: 岡山
• [Book] 先端バイオマテリアル (2012)
  • Author(s): 河野健司
  • Publisher: エヌ・ティー・エス
• [Book] 機能性デンドリマーとＤＤＳ (2012)
  • Author(s): 河野健司
  • Total Pages: 580
  • Publisher: エヌ・ティー・エス
• [Remarks]
  • URL: http://www.chem.osakafu-u.ac.jp/ohka/ohka9/index.htm
• [Remarks] 大阪府大（工）応用化学 生体高分子化学研究室
  • URL: http://www.chem.osakafu-u.ac.jp/ohka/ohka9/index.htm