Development of cell culture methods using sea cucumber collagen fibrils

• Project/Area Number: 23650294
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kogakuin University
• Principal Investigator: IMAMURA Yasutada 工学院大学, 工学部, 教授 (40201339)
• Co-Investigator(Renkei-kenkyūsha): SHIN Yong Chol 工学院大学, 工学部, 准教授 (20291734)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: コラーゲン / コラーゲン線維 / 細胞培養 / ナマコ / 血管内皮細胞 / 骨形成 / 管腔形成 / アパタイト / 石灰化 / 細胞隗 / フィブロネクチン / 血管新生 / 微小血管 / 線維芽細胞

Research Abstract

We have developed newer methods to culture cells by using sea cucumber collagen fibrils as culture substrates. Various conditions were examined for extraction and purification of the collagen fibrils from the body wall dermis in the animal and it became possible to use the fibrils for cell culture. Chemical properties were also demonstrated by showing micro morphology of the fibrils and factors affecting interactions between the fibrils. Some higher order aggregates from the collagen fibrils were formed on the basis of our knowledge about the inter-fibrillar interaction. The collagen fibrils were found to be a good substrate for cell adhesion with various cells examined. Cellular clumps or cellular networks such as capillary like structure were formed under the new culture condition. The collagen fibrils promoted bone differentiation demonstrated by the deposition of apatite with cell culture system. The result indicates the unique surface property of the collagen fibrils.

Research Products

• [Journal Article] Synthesis and evaluation of a cyclopropane derivative of DHMEQ (2014)
  • Author(s): E. Yasui, K. Takayama, T . Nakago, N. Takeda, Y . Imamura, S. Nagumo
  • Journal Title: Chem. Pharm. Bull Volume: 62 Pages: 304-307
  • NAID: 130003381822
• [Journal Article] Synthesis and evaluation of a cyclopropane derivative of DHMEQ (2014)
  • Author(s): Yasui E, Takayama K, Nakago T, Takeda N, Imamura Y, Nagumo S.
  • Journal Title: Chemical and Pharmaceutical Bulletin Volume: 62 Pages: 304-307
  • NAID: 130003381822
  • Peer Reviewed
• [Journal Article] Reconstituted Type V Collagen Fibrils with the Chain Composition of α1(V)α3(V)α3(V) Respective of the D-Periodic Banding Pattern (2013)
  • Author(s): K. Mizuno, Y. Imamura(5人中3番目)
  • Journal Title: Connective Tissue Research Volume: 54(1) Pages: 41-48
• [Journal Article] Fragility of reconstituted type V collagen fibrils with the chain composition of α1(V) α2(V) α3(V) respective of the D-periodic banding pattern (2013)
  • Author(s): K. Mizuno, H. P. Bachinger, Y. Imamura, T. Hayashi, and E. Adachi
  • Journal Title: Connect Tissue Volume: 54 (1) Issue: 1 Pages: 41-48
  • DOI: 10.3109/03008207.2012.734876
  • Peer Reviewed
• [Journal Article] Concerted and adaptive alignment of decorin dermatan sulfate filaments in the graded organization of collagen fibrils in the equine superficial digital flexor tendon (2012)
  • Author(s): T. Watanabe, Y. Imamura(7人中2番目)
  • Journal Title: Journal of Anatomy Volume: 220 Pages: 156-163
• [Presentation] ナマココラーゲン線維によるアパタイトの沈着促進 (2014)
  • Author(s): 五十嵐淳, 今村保忠(6人中6番目)
  • Organizer: 第46回日本結合組織学会・第61回マトリックス研究会大会合同学術集会
  • Place of Presentation: 名古屋
• [Presentation] IV型コラーゲンの3本らせん形成に及ぼす細胞培養条件の影響 (2014)
  • Author(s): 五十嵐淳, 今村保忠(5人中5番目)
  • Organizer: 第46回日本結合組織学会・第61回マトリックス研究会大会合同学術集会
  • Place of Presentation: 名古屋
• [Presentation] Sea Cucumber Collagen Fibrils as Cell Culture Substrates (2013)
  • Author(s): Y . Imamura(他6名)
  • Organizer: 9th Pan Pacific Connective Tissue Societies Symposium
  • Place of Presentation: 香港
• [Presentation] ナマコ由来コラーゲン線維を用いた血管内皮細胞の管腔形成 (2012)
  • Author(s): 新保貴久, 辛英哲, 今村保忠
  • Organizer: 第44回日本結合組織学会・第59回マトリックス研究会大会合同学術集会
  • Place of Presentation: 東京
• [Presentation] ナマコ由来コラーゲン線維を用いた血管内皮細胞の管腔形成 (2012)
  • Author(s): 新保貴久,辛英哲,今村保忠
  • Organizer: 第44回日本結合組織学会学術集会 第59回日本マトリックス研究会大会 合同学術集会
  • Place of Presentation: 東京
• [Presentation] Sea Cucumber Collagen Fibrils As Cell Culture Substrates
  • Author(s): Imamura, Y., Shinbo, T., Amano, A., Kodama, M., Iseki, Y., Miyazaki, H., Shin, Y.
  • Organizer: 9th Pan Pacific Connective Tissue Societies Symposium
  • Place of Presentation: 香港