Molecular basis of epigenetic regulation by ultrasound

• Project/Area Number: 23650303
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Medical systems
• Research Institution: University of Toyama
• Principal Investigator: TABUCHI Yoshiaki 富山大学, 生命科学先端研究センター, 准教授 (20322109)
• Co-Investigator(Kenkyū-buntansha): KONDO Takashi 富山大学, 大学院・医学薬学研究部 (医学), 教授 (40143937) ; WADA Shigehito 富山大学, 大学病院, 講師 (50303219) ; IKEGAME Mika 岡山大学, 医歯(薬)学総合研究科, 准教授 (70282986)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 超音波

Research Abstract

Low-intensity pulsed ultrasound (LIPUS) has been used extensively as an adjuvant to fracture healing. We identified many up-regulated genes that were associated with bone morphology in mouse osteoblast cells treated with a single 20-min LIPUS (1.5 MHz, 30 mW/cm2) using global-scale gene expression analyzing technologies. These results obtained here should help to further clarify the molecular basis of the mechanisms of the LIPUS response in osteoblast cells.

Research Products

• [Journal Article] Development of Oral Epithelial Cell Line ROE2 with Differentiation Potential from Transgenic Rats Harboring Temperature-Sensitive Simian Virus40 Large T-Antigen Gene (2014)
  • Author(s): Tabuchi Y, Wada S, Ikegame M, Kariya A, Furusawa Y, Hoshi N, Yunoki T, Suzuki N, Takasaki I, Kondo T, Suzuki Y
  • Journal Title: Experimental Animals Volume: 63 Issue: 1 Pages: 31-44
  • DOI: 10.1538/expanim.63.31
  • NAID: 130003391601
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] Genes responsive to low-intensity pulsed ultrasound in MC3T3-E1 preosteoblast cells (2013)
  • Author(s): Tabuchi Y, Sugahara Y, Ikegame M, Suzuki N, Kitamura K, Kondo T
  • Journal Title: Int. J. Mol. Sci Volume: Vol.14,No.11 Issue: 11 Pages: 22721-22740
  • DOI: 10.3390/ijms141122721
  • Peer Reviewed
• [Journal Article] Identification of common gene networks responsive to mild hyperthermia in human cancer cells (2013)
  • Author(s): Kariya A, Tabuchi Y, Yunoki T, Kondo T
  • Journal Title: Int. J. Mol. Med Volume: Vol.32,No.1 Issue: 1 Pages: 195-202
  • DOI: 10.3892/ijmm.2013.1366
  • Peer Reviewed
• [Journal Article] Common gene expression patterns responsive to mild temperature hyperthermia in normal human fibroblastic cells. (2013)
  • Author(s): Tabuchi Y, Furusawa Y, Kariya A, Wada S, Ohtsuka K, Kondo T.
  • Journal Title: Int J Hyperthermia Volume: 29 Issue: 1 Pages: 38-50
  • DOI: 10.3109/02656736.2012.753163
  • Peer Reviewed
• [Journal Article] Ultrasound activates ataxia telangiectasia mutated- and Rad-3 related (ATR)-checkpoint kinase 1 (Chk1) pathway in human leukemia Jurkat cells (2012)
  • Author(s): Furusawa Y, Iizumi T, Fujiwara Y, Hassan MA, Tabuchi Y, Nomura T, and Kondo T
  • Journal Title: Ultrason Sonochem Volume: 19 Issue: 6 Pages: 1246-1251
  • DOI: 10.1016/j.ultsonch.2012.04.003
  • Peer Reviewed
• [Journal Article] Inhibition of DNA-dependent protein kinase promotes ultrasound -induced cell death including apoptosis in human leukemia cells (2012)
  • Author(s): Furusawa Y, Fujiwara Y, Hassan MA, Tabuchi Y, Morita A, Enomoto A,and Kondo T
  • Journal Title: Cancer Lett Volume: 322 Issue: 1 Pages: 107-112
  • DOI: 10.1016/j.canlet.2012.02.020
  • Peer Reviewed
• [Journal Article] Gene networks related to the cell death elicited by hyperthermia (2012)
  • Author(s): Tabuchi Y
  • Journal Title: Int J Mol Med Volume: 29 Pages: 380-386
  • DOI: 10.3892/ijmm.2011.862
  • Peer Reviewed
• [Journal Article] DNA double-strand breaks induced by cavitational mechanical effects of ultrasound in cancer cell lines (2012)
  • Author(s): Furusawa Y., Y. Fujiwara, P. Campbell, Q. L. Zhao, R. Ogawa, M. A. Hassan, Y. Tabuchi, I. Takasaki, A. Takahashi, T. Kondo
  • Journal Title: PLoS ONE Volume: 7 Issue: 1 Pages: e29012-e29012
  • DOI: 10.1371/journal.pone.0029012
  • Peer Reviewed
• [Journal Article] Indetification of biological functions and gene networks regulated by heat stress in U937 human lymphoma cells (2011)
  • Author(s): Furusawa Y., et al.
  • Journal Title: Int.J.Mol.Med. Volume: 28 Pages: 143-151
  • DOI: 10.3892/ijmm.2011.702
  • Peer Reviewed
• [Presentation] MC3T3-E1 前骨芽細胞様細胞において低出力パルス超音波に応答する遺伝子の同定 (2013)
  • Author(s): 田渕圭章, 菅原有希, 池亀美華, 鈴木信雄, 北村敬一郎, 近藤 隆
  • Organizer: 第12回超音波治療研究会
  • Place of Presentation: 東京
  • Year and Date: 2013-11-30
• [Presentation] Identification of genes responsive to low-intensity pulsed ultrasound in mouse preosteoblast cells (2013)
  • Author(s): Tabuchi Y, Sugahara Y, Ikegame M, Suzuki N, Kitamura KI, Kondo T
  • Organizer: Int. Conference on Biomed. Ultrasound 2013
  • Place of Presentation: Taipei
• [Presentation] MC3T3-E1前骨芽細胞様細胞において低出力パルス超音波に応答する遺伝子の同定 (2013)
  • Author(s): 田渕圭章，菅原有希，池亀美華，鈴木信雄，北村敬一郎，近藤 隆
  • Organizer: 第12回超音波治療研究会
  • Place of Presentation: 東京
• [Remarks]
  • URL: http://www.lsrc.u-toyama.ac.jp/mgrc/html/laboratory.html