| Project/Area Number |
23650337
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Tokai University |
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Principal Investigator |
TSUDA Michio 東海大学, 医学部, 非常勤教授 (00102848)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | リハビリテーション医学 / 筋萎縮 / 血流 / ヒラメ筋 / 長指伸筋 / 遺伝子発現 / 廃用性萎縮 / ギプス / RNA解析 |
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| Research Abstract |
We experimented on rat model to simulate disuse atrophy, analyzed the blood flow and the gene expression pattern in the model utilizing immobilization. Male Wistar rats, aged 20 weeks, were used in this study. 10days before the experiment day, right hind-limbs of all rats were immobilized in a plaster cast (left contra-lateral hind-limbs were analyzed as controls). On the experiment day, all rats were anesthetized and muscles (extensor digitorum longus muscle: EDL, and Soleus mucle: SM) were removed and weighed. Muscles were prepared by documented method and were analyzed for gene expression pattern difference on DNA arrays (Agilent Technology Inc.). 10days immobilization resulted in significant decrease of muscles weight in right (immobilized) muscles. This was more pronounced in SM than in EDL. This was also observed in DNA array analysis and Real-time PCR.
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