Bioimaging technique to observe skeletal muscle metabolites in vivo

• Project/Area Number: 23650403
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Sports science
• Research Institution: The University of Electro-Communications
• Principal Investigator: KANO Yutaka 電気通信大学, 情報理工学研究科, 教授 (90293133)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: バイオイメージング / 骨格筋 / ホメオスタシス / 筋収縮 / ミトコンドリア / 細胞内pH

Research Abstract

Using an in vivo bioimaging model and microinjection technique, we tested homeostasis of intracellular when low pH solution was loaded in single fiber by microinjection. The intact spinotrapezius muscle of adult male Wistar rats was exteriorized and loaded with the fluorescent probe BCECF-AM. Muscle was loaded low pH solution by microinjection. This model offers a unique opportunity to investigate the consequences of experimental manipulations of pH on muscle buffering dynamics under close-to-physiological conditions

Research Products

• [Journal Article] Mechanisms of exercise-induced muscle damage and fatigue: Intracellular calcium accumulation (2012)
  • Author(s): Yutaka Kano, Takashi Sonobe, Tadakatsu Inagaki, Mizuki Sudo and David C Poole
  • Journal Title: The Journal of Physical Fitness and Sports Medicine Volume: 1 Issue: 3 Pages: 505-512
  • DOI: 10.7600/jpfsm.1.505
  • NAID: 10031158982
  • ISSN: 2186-8123, 2186-8131
  • Peer Reviewed
• [Presentation] In vivo measurement of intercellular pH and contractile performance during repetitive stimulation in rat skeletal muscle (2012)
  • Author(s): Yoshinori Tanaka, Tadakatsu Inagaki, Takuma Kimura, David C Poole, Yutaka Kano
  • Organizer: Med. Sci. Sport Exer
  • Place of Presentation: San Francisco, USA
• [Presentation] In vivo imaging of intracellular sodium ion following eccentric contractions in rat skeletal muscle (2012)
  • Author(s): Takuma Kimura, Tadakatsu Inagaki, Yoshinori Tanaka, David C Poole, Yutaka Kano
  • Organizer: Med. Sci. Sport Exer
  • Place of Presentation: San Francisco, USA
• [Presentation] In vivo measurement of intercellular pH and contractile performance during repetitive stimulation in rat skeletal muscle (2012)
  • Author(s): Yoshinori Tanaka, Tadakatsu Inagaki, Takuma Kimura, David C Poole, Yutaka Kano
  • Organizer: The Systems Biology of Exercise: Cardiorespiratory & Metabolic Integration
  • Place of Presentation: Univ. of Leeds
• [Presentation] 筋細胞内ナトリウムイオンに対する血流の影響 (2012)
  • Author(s): 木村卓磨,稲垣薫克,田中嘉法,狩野豊
  • Organizer: 第67回体力医学会
  • Place of Presentation: 岐阜
• [Presentation] Buffering capacity of single muscle fiber under in vivo environment (2012)
  • Author(s): Yoshinori Tanaka, Tadakatsu Inagaki, Takuma Kimura, David C Poole, Yutaka Kano
  • Organizer: The Systems Biology of Exercise: Cardiorespiratory & Metabolic Integration
  • Place of Presentation: University of Leeds, UK
  • Invited
• [Presentation] 筋収縮時の筋細胞内pH変化に対する膜輸送体の関与 (2012)
  • Author(s): 田中嘉法，稲垣薫克，木村卓磨，狩野豊
  • Organizer: 第６７回日本体力医学会
  • Place of Presentation: 岐阜
• [Presentation] 筋細胞内Na+に対する血流の影響 (2012)
  • Author(s): 木村卓磨，田中嘉法，狩野豊
  • Organizer: 第６７回日本体力医学会
  • Place of Presentation: 岐阜
• [Presentation] 筋収縮時の筋細胞内pH変化に対する膜輸送体の関与 (2011)
  • Author(s): 田中嘉法,稲垣薫克,木村卓磨,狩野豊
  • Organizer: 第67回体力医学会
  • Place of Presentation: 岐阜
• [Presentation] 単一筋線維レベルでのinvivopHイメージング (2011)
  • Author(s): 田中嘉法,稲垣薫克,木村卓磨,狩野豊
  • Organizer: 第66回体力医学会
  • Place of Presentation: 山口
• [Presentation] 伸張性筋収縮後の細胞内ナトリウムイオンの可視化 (2011)
  • Author(s): 木村卓磨,稲垣薫克,田中嘉法,狩野豊
  • Organizer: 第66回体力医学会
  • Place of Presentation: 山口
• [Presentation] 単一筋線維レベルでのin vivo pHイメージング (2011)
  • Author(s): 田中嘉法，稲垣薫克，木村卓磨，狩野豊
  • Organizer: 第66回 日本体力医学会
  • Place of Presentation: 山口県，海峡メッセ下関