Bioimaging technique to observe skeletal muscle metabolites in vivo
| Project/Area Number |
23650403
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Sports science
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| Research Institution | The University of Electro-Communications |
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Principal Investigator |
KANO Yutaka 電気通信大学, 情報理工学研究科, 教授 (90293133)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | バイオイメージング / 骨格筋 / ホメオスタシス / 筋収縮 / ミトコンドリア / 細胞内pH |
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| Research Abstract |
Using an in vivo bioimaging model and microinjection technique, we tested homeostasis of intracellular when low pH solution was loaded in single fiber by microinjection. The intact spinotrapezius muscle of adult male Wistar rats was exteriorized and loaded with the fluorescent probe BCECF-AM. Muscle was loaded low pH solution by microinjection. This model offers a unique opportunity to investigate the consequences of experimental manipulations of pH on muscle buffering dynamics under close-to-physiological conditions
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Report
(3 results)
Research Products
(12 results)
