| Project/Area Number |
23650407
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Sports science
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| Research Institution | Tokai Gakuen University (2012)
Aichi Prefectural University (2011) |
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Principal Investigator |
KANAO Yoji 東海学園大学, スポーツ健康科学部, 教授 (10177488)
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| Co-Investigator(Renkei-kenkyūsha) |
KUROSU Masahiro 東海学園大学, スポーツ健康科学部, 助教 (60469054)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 運動性貧血 / 赤血球膜 / 膜脆弱性 / 浸透圧変化 / 二重遠心分離 / SDS-PAGE / 質量分析法 / LC-MS/MS分析 / ウエスタンブロッティング / アンキリン / バンド3 |
|---|
| Research Abstract |
The purpose of this study is to clarify the differences between fragile red cells and not fragile ones through the differences of proteins which compose those red cell. In the analysis of SDS-PAGE , thick bands are found in tough red cells in four bands such as 120 ,80 , 66 , 60 , (kDa). Under the analysis of LC-MS/MS , ankyrin is clearly found . Thus that result suggests that the red cell membrane is fragile due to loss of a part of ankyrin.
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