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Effect of Eccentric Contraction on Satellite Cell Activation in Human Vastus Lateralis Muscle

Research Project

Project/Area Number 23650434
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied health science
Research InstitutionYamaguchi University

Principal Investigator

MIYATA Hirofumi  山口大学, 医学(系)研究科(研究院), 教授 (90190793)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsエキセントリック収縮 / サテライトセル / 活性化サイトカイン / 外側広筋 / HGF / 抗重力筋
Research Abstract

We investigated the time-course of SC activation in vastus lateralis (VL) muscle after step exercise. Eleven non-trained young adults participated in a 30-minute step up/down exercise protocol involving mainly concentric contractions with right leg and mainly eccentric contractions with left leg. Biopsy samples were taken from the both side of VL muscles before exercise, and 2 and 5 days after exercise. The numbers of SCs for each fiber type were determined by using immunofluorescence staining. The expressions of several mRNA were analyzed using real-time RT-PCR system. The numbers of SCs in both side VL muscles did not changed in all fiber types after exercise. The expressions of HGF mRNA increased significantly at 2-5 days and the expression of myostatin mRNA also increased at 5 days after exercise in left VL, but not in right VL muscles. The expressions of IL-6, MyoD, and IGF-I mRNA slightly increased in left VL, but not in right VL muscles after exercise.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (11 results)

All 2014 2013 2012 2011

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (8 results)

  • [Journal Article] 競走馬のトレーニングおよび脱トレーニングが骨格筋サテライトセルの反応性に及ぼす影響2014

    • Author(s)
      今岡洋子, 河合美菜子, 向井和隆, 大村一, 高橋敏之, 平賀敦, 宮田浩文
    • Journal Title

      体力科学

      Volume: 63(1) Pages: 177-187

    • NAID

      130003394102

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Training and Detraining Effects on Satellite Cell Response after Exhaustive Exercise in Thoroughbred Horses2014

    • Author(s)
      今岡洋子
    • Journal Title

      Japanese Journal of Physical Fitness and Sports Medicine

      Volume: 63 Issue: 1 Pages: 177-187

    • DOI

      10.7600/jspfsm.63.177

    • NAID

      130003394102

    • ISSN
      0039-906X, 1881-4751
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 筋力トレーニングとしてのロングストライド歩行の可能性2011

    • Author(s)
      丸山昭男, 久津見学, 宮田浩文
    • Journal Title

      臨床スポーツ医学

      Volume: 28(4) Pages: 469-475

    • NAID

      40018773589

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Effect of Eccentric Contraction on Satellite Cell Activation in Human Vastus Lateralis Muscle2014

    • Author(s)
      Miyata H, Imaoka Y, Kawai M, Mori F
    • Organizer
      第61回アメリカスポーツ医学会
    • Place of Presentation
      オーランド(USA)
    • Year and Date
      2014-05-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Effect of Eccentric Contraction on Satellite Cell Activation in Human Vastus Lateralis Muscle2014

    • Author(s)
      Hirofumi Miyata
    • Organizer
      第61回アメリカスポーツ医学会
    • Place of Presentation
      オーランド(USA)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 踏み台昇降エキセントリック収縮による骨格筋サテライトセルの活性化2013

    • Author(s)
      今岡洋子, 福田智亮, 河合美菜子, 森大志, 宮田浩文
    • Organizer
      第72回日本体力医学会中国四国地方会
    • Place of Presentation
      ふれあいの里(米子市)
    • Year and Date
      2013-11-10
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒトの骨格筋線維特性は他の哺乳類と異なるか2013

    • Author(s)
      宮田浩文
    • Organizer
      第21回日本運動生理学会
    • Place of Presentation
      東京国際大学(川越市)
    • Year and Date
      2013-07-28
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト外側広筋微量サンプルのmRNA発現相対量を用いた筋線維組成の推定2013

    • Author(s)
      福田智亮
    • Organizer
      第72回日本体力医学会中国四国地方会
    • Place of Presentation
      ふれあいの里(米子市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 競走馬のミオスタチン遺伝子多型骨格筋線維特性2013

    • Author(s)
      菊野理佳
    • Organizer
      第72回日本体力医学会中国四国地方会
    • Place of Presentation
      ふれあいの里(米子市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒト外側広筋微量サンプルのmRNA 発現相対量を用いた筋線維組成の推定2012

    • Author(s)
      宮田浩文, 河合美菜子, 森大志
    • Organizer
      第70回日本体力医学会中国四国地方会
    • Place of Presentation
      吉備国際大(高梁市)
    • Year and Date
      2012-11-25
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト外側広筋微量サンプルのmRNA発現相対量を用いた筋線維組成の推定2012

    • Author(s)
      宮田浩文
    • Organizer
      第69回日本体力医学会、中国四国地方会
    • Place of Presentation
      吉備国際大学(高梁市)
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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