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Analysis of all forms of vitamin B6 contents in foods by new enzyme-HPLC method

Research Project

Project/Area Number 23650483
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Eating habits, studies on eating habits
Research InstitutionKochi University

Principal Investigator

YAGI Toshiharu  高知大学, 教育研究部自然科学系, 教授 (90110759)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsビタミンB6 / 食品 / 母乳 / 味噌 / 寿司ネタ / 刺身 / 糖尿病合併症 / 食品成分表
Research Abstract

Contents of six forms and a storage form of vitamin B6 in foods were determined by the highly sensitive method (new enzyme-HPLC method). The conditions for preparation of analytical samples of foods were firstly developed. The contents in several animal and plant foods were determined. Then, some sashimi used for sushi toppings, fermented soy bean foods, and Japanese women’s milk were analyzed.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (23 results)

All 2013 2012 2011

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (9 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] Contents of all forms of vitamin B6, pyridoxine-β-glucoside and 4-pyridoxic acid in mature milk of Japanese women by 4-pyridoxolactone-conversion high performance chromatography2013

    • Author(s)
      Yagi T., Iwamoto S., Mizuseki R., Furuya M. Nakayama K.
    • Journal Title

      J. Nutr. Sci. Vitaminol

      Volume: Vol. 59, No.1 Pages: 9-15

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of pyridoxine 4-oxidase from Mesorhizobium loti2013

    • Author(s)
      Mugo A.N., Kobayashi J., Yamasaki T., Mikami B., Ohnishi K., Yoshikane Y., Yagi T
    • Journal Title

      Biochim. Biophys. Acta -Proteins and Protemics

      Volume: Vol. 1834 Pages: 953-963

    • URL

      http://dx.doi.org/10.1016/j.bbapap.2013.03.004

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Contents of all forms of vitamin B6, pyridoxine-β-glucoside and 4-pyridoxic acid in mature milk of Japanese women by 4-pyridoxolactone-conversion high performance chromatography.2013

    • Author(s)
      Yagi T., Iwamoto S., Mizuseki R., Furuya M., Nakayama K.
    • Journal Title

      J. Nutr. Sci. Vitaminol.

      Volume: 59 Pages: 9-15

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of pyridoxine 4-oxidase from Mesorhizobium loti.2013

    • Author(s)
      Mugo A.N., Kobayashi J., Yamasaki T., Mikami B., Ohnishi K., Yoshikane Y., Yagi T.
    • Journal Title

      Biochim. Biophys. Acta

      Volume: in press Issue: 6 Pages: 953-963

    • DOI

      10.1016/j.bbapap.2013.03.004

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] All-enzymatic HPLC method for determination of individual and total contents of vitamin B6 in foods.2012

    • Author(s)
      Do H.T.V., Ide Y., Mugo A.N., Yagi T.
    • Journal Title

      Food & Nutr. Res.

      Volume: 56 Issue: 1 Pages: 5409-5417

    • DOI

      10.3402/fnr.v56i0.5409

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Individual vitamin B6 contents in selected Japanese sushi toppings.2012

    • Author(s)
      Do H.T.V., Yagi, T.
    • Journal Title

      Int. J. Vitam. Nutr. Res.

      Volume: 63 Issue: 2 Pages: 249-249

    • DOI

      10.3109/09637486.2011.639350

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Abnormality in expression levels of gluconeogenesis-related genes by high-dose supplementation with pyridoxamine in mice2012

    • Author(s)
      Do, H.T.V., Toda, K., Saibara, T., Yagi, T.
    • Journal Title

      Int. J. Vitam. Nutr. Res

      Volume: Vol. 82, No. 1 Issue: 1 Pages: 30-40

    • DOI

      10.1024/0300-9831/a000092

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The mll6786 gene encodes a repressor protein controlling the degradation pathway for vitamin B6 in Mesorhizobium loti.2012

    • Author(s)
      Nagase T., Mugo A.N., Chu H.N., Yoshikane Y., Ohnishi K., Yagi T.
    • Journal Title

      FEMS Microbiol. Lett.

      Volume: 329 Issue: 2 Pages: 116-122

    • DOI

      10.1111/j.1574-6968.2012.02510.x

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Abnormality in expression levels of gluconeogenesis-related genes by high-dose supplementation with pyridoxamine in mice.2012

    • Author(s)
      Do, H.T.V., Toda, K., Saibara, T., Yagi, T.
    • Journal Title

      Int. J. Vitam. Nutr. Res.

      Volume: 82 Pages: 34-40

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] All-enzymatic HPLC method for determination of individual and total contents of vitamin B6 in foods.2012

    • Author(s)
      Do, H. T. V., Y. Ide, Mugo, A. N., Yagi, T.
    • Journal Title

      Food & Nutrition Research

      Volume: 55 Pages: 5409-5416

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] ビタミンB6測定法の現状と課題2011

    • Author(s)
      八木年晴
    • Journal Title

      ビタミン

      Volume: 85 Pages: 116-126

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of pyridoxine 4-oxidase, the first enzyme in pyridoxine degradation pathway I2011

    • Author(s)
      Mugo A.N., Kobayashi J., Mikami B., Ohnishi K., Yagi T.
    • Journal Title

      Acta Crystallographica Section F

      Volume: 68 Issue: 1 Pages: 66-68

    • DOI

      10.1107/s1744309111048688

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Crystal structure of pyridoxine 4-oxidase from Mesorhizobium loti2013

    • Author(s)
      Mugo Andrew Njagi
    • Organizer
      日本農芸化学会中四国支部第35回講演会(例会)
    • Place of Presentation
      高知大学
    • Year and Date
      2013-01-26
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] ピリドキシン 4-オキシダーゼの活性中心残基とその変異2013

    • Author(s)
      渡邊江里子
    • Organizer
      日本農芸化学会中四国支部第35回講演会(例会)
    • Place of Presentation
      高知大学
    • Year and Date
      2013-01-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 4-ピリドキシン酸転換HPLC法によるヒト赤血球・血漿中のピリドキサール・ピリドキサール 5'-リン酸定量条件の検討と乳中 4-ピリドキシン酸定量法2013

    • Author(s)
      水関梨江
    • Organizer
      日本農芸化学会中四国支部第35回講演会(例会)
    • Place of Presentation
      高知大学
    • Year and Date
      2013-01-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 大豆発酵食品中の全ビタミンB6 化合物含有量2013

    • Author(s)
      大下浩子
    • Organizer
      日本農芸化学会中四国支部第35回講演会(例会)
    • Place of Presentation
      高知大学
    • Year and Date
      2013-01-26
    • Related Report
      2012 Final Research Report
  • [Presentation] ビタミンB6酵素の基礎と応用2013

    • Author(s)
      八木年晴
    • Organizer
      日本農芸化学会中四国支部第35回講演会(例会)
    • Place of Presentation
      高知大学(招待講演)
    • Year and Date
      2013-01-26
    • Related Report
      2012 Final Research Report
  • [Presentation] ピリドキシン4-オキシダーゼの活性中心残基とその変異2013

    • Author(s)
      渡邊江里子
    • Organizer
      日本農芸化学会中四国支部第35回講演会(例会)
    • Place of Presentation
      高知大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 4-ピリドキシン酸転換HPLC法によるヒト赤血球・血漿中のピリドキサール・ピリドキサール5'-リン酸定量条件の検討と乳中4-ピリドキシン酸定量法2013

    • Author(s)
      水関梨江
    • Organizer
      日本農芸化学会中四国支部第35回講演会(例会)
    • Place of Presentation
      高知大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 大豆発酵食品中の全ビタミンB6化合物含有量2013

    • Author(s)
      大下浩子
    • Organizer
      日本農芸化学会中四国支部第35回講演会(例会)
    • Place of Presentation
      高知大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ビタミンB6酵素の基礎と応用2013

    • Author(s)
      八木年晴
    • Organizer
      日本農芸化学会中四国支部第35回講演会(例会)
    • Place of Presentation
      高知大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] The Royal Society of Chemistry, Enzymatic HPLC assay for all six vitamin B6 forms. In Food and Nutritional Components in Focus No. 4 B Vitamins and Folate: Chemistry, Analysis, Function and Effects2013

    • Author(s)
      Yagi T
    • Related Report
      2012 Final Research Report
  • [Book] Enzymatic HPLC assay for all six vitamin B6 forms. In Food and Nutritional Components in Focus No. 4 B Vitamins and Folate: Chemistry, Analysis, Function and Effects2013

    • Author(s)
      Yagi T.
    • Publisher
      The Royal Society of Chemistry
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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