Chemical analyses of microflora and feces in lifestyle-related disease animals for the identification of intestinal inflammatory biomarkers
| Project/Area Number |
23650486
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | University of Shizuoka |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 健康と食生活 / 炎症 / 生活習慣病 / 質量分析 / バイオマーカー |
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| Research Abstract |
Recent pioneering studies have revealed that intestinal flora was an important factor to determine the host phenotype associated with lifestyle-related diseases. Moreover low level but continuous inflammation derived from the alteration of intestinal flora appears to contribute to the pathogenesis of these serious diseases. Therefore, in this study, we performed chemical and statistical analysis of intestinal flora and feces to identify the inflammation biomarkers. We examined conditions for the breeding, sample preparations, MS analyses, multivariate analyses, and etc. Using optimum condition, harvested samples were subjected to UPLC-TOF-MS or GC-EI-MS. Several MS ion peaks were detected as a candidate for inflammation biomarker. Based on these results, further study is in progress to identify inflammation biomarker in intestinal tract.
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Report
(3 results)
Research Products
(47 results)
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[Journal Article] Development and application of a method for identification of isothiocyanate-targeted molecules in colon cancer cells2012
Author(s)
Miyoshi, N., Yonemochi, T., Tomono, S., Fukutomi, R., Nakamura, Y., Ohshima, H.
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Journal Title
Anal. Biochem.
Volume: 429(2)
Issue: 2
Pages: 124-131
DOI
Related Report
Peer Reviewed
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