Establishment of simple detection method of invasive ant species applying DNA bar coding and application of environmental education

• Project/Area Number: 23650494
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Science education
• Research Institution: Hokkaido University of Education
• Principal Investigator: MURAKAMI Takahiro 北海道教育大学, 教育学部, 准教授 (40374706)
• Co-Investigator(Kenkyū-buntansha): MIYATA Hiroki 株式会社竹中工務店, 技術研究所, 研究員 (90416628)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: DNA bar coding / 簡易PCRマシーン / 外来アリ / 環境教育 / PCR / 多様性 / 理科教育 / アリ / DNAバーコーディング / 外来種 / CAP-PCR / 簡易PCR機器 / アリ類 / モニタリング / バイオロギング

Research Abstract

According to this research, we could establish a simple and useful PCR device. Until then, the cheapest thermal cycler is around 300,000 Japanese yen, but this new developed PCR device is only 100,000 (in fact 80,000 yen). This is the most valuable accomplishment in our study. Furthermore, this newly established device could detect unique DNA bar cording band patterns of invasive ant species derived from Central America. This is much easier than that of typical DNA bar coding using DNA sequencing process. We implemented to the Sapporo Ohtani high school, Hokkaido Science Educational Center, and Chiba Oihama high school with its teachers and it confirmed that this device and experimental procedure are fully effective against environmental education in high school curriculum.

Research Products

• [Journal Article] ポーラ美術館における虫害管理について (2013)
  • Author(s): 宮田弘樹
  • Journal Title: 文化財の虫被害 Volume: 64巻 Pages: 20-23
  • NAID: 40019548115
• [Journal Article] ポーラ美術館における虫害管理について (2013)
  • Author(s): 宮田弘樹
  • Journal Title: 文化財の虫菌害 Volume: 64 Pages: 20-23
  • NAID: 40019548115
• [Presentation] Isolation and invasion of Siberian chipmunks (Tamias sibiricus) in Mt. Hakodate, Hokkaido (2014)
  • Author(s): Iwashita K., and Murakmai T
  • Organizer: 第61回日本生態学会
  • Place of Presentation: 広島県広島市
  • Year and Date: 2014-03-16
• [Presentation] Where have the Cervus nippon in Southern Hokkaido been coming from? - genetic structures, distirbutions,and conservation ecology- (2014)
  • Author(s): Honda H., and Murakami T
  • Organizer: 第61回日本生態学会
  • Place of Presentation: 広島県広島市
  • Year and Date: 2014-03-16
• [Presentation] 防虫エンジニアリングによる異物混入防止対策 (2013)
  • Author(s): 宮田弘樹
  • Organizer: 最新のフードセーフティ情報
  • Place of Presentation: 東京都千代田区
  • Year and Date: 2013-06-21
• [Presentation] The kin recognition behaviors and genetic structures of 8 Argentine ant (Linepithema humile) super colonies in western Japan (2013)
  • Author(s): Sato K., Sakamoto H., Kobayashi M., Ozaki M., Higashi S., and Murakami T
  • Organizer: 第60回生態学会
  • Place of Presentation: 静岡県静岡市
  • Year and Date: 2013-03-06
• [Presentation] The kin recognition behaviors and genetic structures of 8 Argentine ant (Linepithema humile) super colonies in western Japan (2013)
  • Author(s): Kazuki Sato, Hironori Sakamoto, MIdori Kobayashi, Mamiko Ozaki , Seigo Higashi, Takahiro Murakami
  • Organizer: 第60回日本生態学会
  • Place of Presentation: 静岡県静岡市
• [Presentation] DNA バーコーディング法を応用したアリ種の簡易検出法の開発と生物教育への応用 (2012)
  • Author(s): 佐藤一樹、宮田弘樹、村上貴弘
  • Organizer: 第62回理科教育学会
  • Place of Presentation: 鹿児島県鹿児島市
  • Year and Date: 2012-08-11
• [Presentation] Kin recognition behaviors and genetic diversity of invasive ant, Linepithema humile in Japan and Argentina (2012)
  • Author(s): Sato K., Sakamoto H., Hirata M., Kamada S., Higashi S., and Murakami T
  • Organizer: Joint meeting of the 59^<th> annual meeting of ESJ and the 5^<th> EAFES international congress
  • Place of Presentation: 龍谷大学(滋賀県大津市)
  • Year and Date: 2012-03-19
• [Presentation] 種の多様性を規定する都市の景観構造 (2012)
  • Author(s): 宮田弘樹、伊勢紀、三輪隆、北野正人、三橋弘宗、伊東憲正、鈴木明子
  • Organizer: Joint meeting of the 59^<th> annual meeting of ESJ and the 5^<th> EAFES international congress
  • Place of Presentation: 龍谷大学(滋賀県大津市)
  • Year and Date: 2012-03-18
• [Presentation] Unusual chromosome numbers and polyploidy in invasive fire ant populations (2012)
  • Author(s): Murakami T
  • Organizer: Joint meeting of the 59^<th> annual meeting of ESJ and the 5^<th> EAFES international congress
  • Place of Presentation: 龍谷大学(滋賀県大津市)
  • Year and Date: 2012-03-18
• [Presentation] DNAバーコーディング法を応用したアリ種の簡易検出法の開発と生物教育への応用 (2012)
  • Author(s): 佐藤一樹，宮田弘樹，村上貴弘
  • Organizer: 第62回理科教育学会
  • Place of Presentation: 鹿児島県鹿児島市
• [Presentation] Unusual chromosome numbers and polyploidy in invasive fire ant populations. (2012)
  • Author(s): Murakami Takahiro
  • Organizer: Joint meeting of the 59th annual meeting of ESJ and the 5th EAFES international congress
  • Place of Presentation: 龍谷大学（滋賀県大津市）
• [Presentation] Kin recognition behaviors and genetic diversity of invasive ant, Linepithema humile in Japan and Argentina. (2012)
  • Author(s): Sato Kazuki, Sakamoto Hironori, Hirata Masanori, Kamada Shoko, Higashi Seigo, and Murakami Takahiro
  • Organizer: Joint meeting of the 59th annual meeting of ESJ and the 5th EAFES international congress
  • Place of Presentation: 龍谷大学（滋賀県大津市）
• [Presentation] 種の多様性を規定する都市の景観構造 (2012)
  • Author(s): 宮田弘樹、伊勢紀、三輪隆、北野正人、三橋弘宗、伊東憲正、鈴木明子
  • Organizer: Joint meeting of the 59th annual meeting of ESJ and the 5th EAFES international congress
  • Place of Presentation: 龍谷大学（滋賀県大津市）
• [Presentation] Isolation and invasion of Siberian Chipmunks (Tamias sibiricus) in Mt.Hakodate, Hokkaido.
  • Author(s): Kumari Iwashita, Takahiro Murakami
  • Organizer: ESJ61
  • Place of Presentation: 広島県広島市
• [Presentation] Where have the Cervus nippon in Southern Hokkaido been coming from? ~genetic structures, distributions, and conservation ecology~
  • Author(s): Honda Hiroshi, Takahiro Murakami
  • Organizer: ESJ61
  • Place of Presentation: 広島県広島市
• [Presentation] 防虫エンジニアリング』による異物混入防止対策
  • Author(s): 宮田弘樹
  • Organizer: 最新のフードセーフティ情報
  • Place of Presentation: 東京都千代田区
  • Invited
• [Book] アリの生物学 (2015)
  • Author(s): 村上貴弘
  • Total Pages: 300
  • Publisher: 東海大学出版会
• [Book] アリの生物学 (2014)
  • Author(s): 坂本洋典、村上貴弘, 編著
  • Total Pages: 300
  • Publisher: 東海大学出版会
• [Book] パワー・エコロジー (2013)
  • Author(s): 佐藤宏明、村上貴弘, 編著
  • Total Pages: 480
  • Publisher: 海游舎
• [Book] パワー・エコロジー (2013)
  • Author(s): 村上貴弘
  • Total Pages: 480
  • Publisher: 海游舎