| Project/Area Number |
23650494
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science education
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| Research Institution | Hokkaido University of Education |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MIYATA Hiroki 株式会社竹中工務店, 技術研究所, 研究員 (90416628)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | DNA bar coding / 簡易PCRマシーン / 外来アリ / 環境教育 / PCR / 多様性 / 理科教育 / アリ / DNAバーコーディング / 外来種 / CAP-PCR / 簡易PCR機器 / アリ類 / モニタリング / バイオロギング |
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| Research Abstract |
According to this research, we could establish a simple and useful PCR device. Until then, the cheapest thermal cycler is around 300,000 Japanese yen, but this new developed PCR device is only 100,000 (in fact 80,000 yen). This is the most valuable accomplishment in our study. Furthermore, this newly established device could detect unique DNA bar cording band patterns of invasive ant species derived from Central America. This is much easier than that of typical DNA bar coding using DNA sequencing process. We implemented to the Sapporo Ohtani high school, Hokkaido Science Educational Center, and Chiba Oihama high school with its teachers and it confirmed that this device and experimental procedure are fully effective against environmental education in high school curriculum.
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