Establishment of screening system for the candidate compounds for anti-cancer drugs with iPS cell
Project/Area Number |
23650587
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Carcinogenesis
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Research Institution | Tohoku University |
Principal Investigator |
FUKUDA Tomokazu 東北大学, (連合)農学研究科(研究院), 准教授 (40321640)
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | エピジェネティクス / 人工多能性幹細胞 / ゲノム / サイレンシング / 脱メチル化 |
Research Abstract |
We focused on the common biological feature between cancer and iPS cell, and tried to establish the screening system for de-methylating agents for the genome. We introduced reprogramming four factors into E6/E7 immortalized mouse embryonic fibroblasts (MEF). The infected MEF showed iPS transformation. We successfully established mouse iPS cells, and found that reported activity is dramatically dropped down. We are trying to screen the mow molecular compounds, which causes the re-activation of the promoter activity.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Efficient establishment of pig embryonic fibroblast cell lines with conditional expression of simian vacuolating virus 40 large T fragment.2012
Author(s)
Fukuda, T., Katayama, M., Yoshizawa, T., Eitsuka, T., Mizukami, H., Nakagawa, K., Ito, H., Komagata, H., Song, S., Roh, S., Sato, E., Hanada, H., Nishimori, K., Miyazawa, T., and Uchida, T
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Journal Title
Bioscience Biotechnology and Biochemistry
Volume: in press
NAID
Related Report
Peer Reviewed
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[Journal Article] Antiproliferative effect of cathelicidin family peptide LL-37 analogue on colon cancer HCT116 p53+/+ and p53-/- cells.2012
Author(s)
Kuroda, K., Fukuda, T., Yoneyama, H., Katayama, M., Isogai, H., Okumura, K., and Isogai, M
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Journal Title
Oncology Rep
Volume: in press
Related Report
Peer Reviewed
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