Establishment of screening system for the candidate compounds for anti-cancer drugs with iPS cell

• Project/Area Number: 23650587
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Carcinogenesis
• Research Institution: Tohoku University
• Principal Investigator: FUKUDA Tomokazu 東北大学, (連合)農学研究科(研究院), 准教授 (40321640)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: エピジェネティクス / 人工多能性幹細胞 / ゲノム / サイレンシング / 脱メチル化

Research Abstract

We focused on the common biological feature between cancer and iPS cell, and tried to establish the screening system for de-methylating agents for the genome. We introduced reprogramming four factors into E6/E7 immortalized mouse embryonic fibroblasts (MEF). The infected MEF showed iPS transformation. We successfully established mouse iPS cells, and found that reported activity is dramatically dropped down. We are trying to screen the mow molecular compounds, which causes the re-activation of the promoter activity.

Research Products

• [Journal Article] Low-molecular-weight inhibitors of cell differentiation enable efficient growth of mouse iPS cells under feeder-free conditions (2014)
  • Author(s): Donai K, Inagaki A, So KH, Kuroda K, Sone H, Kobayashi M, Nishimori K, Fukuda T
  • Journal Title: Cytotechnology Volume: (in press) Issue: 2 Pages: 191-197
  • DOI: 10.1007/s10616-013-9686-8
  • Peer Reviewed
• [Journal Article] Low-Molecular-Weight Inhibitors of Cell Differentiation Enable Efficient Growth of Mouse iPS Cells under Feeder-Free Conditions (2014)
  • Author(s): Donai, K. Inagaki, A. So, HK. Kuroda, K. Sone, H. Kobayashi, M. Nishimori, K. Fukuda, T
  • Journal Title: Cytotechnology Volume: in press
  • Peer Reviewed
• [Journal Article] Establishment of a Reporter System to Monitor Silencing Status in induced Pluripotent Stem Cell Lines (2013)
  • Author(s): Donai K, Kuroda K, Guo Y, So K-H, Sone H, Kobayashi M, Nishimori K and Fukuda T
  • Journal Title: Anal Biochem Volume: 443 Issue: 1 Pages: 104-112
  • DOI: 10.1016/j.ab.2013.08.014
  • Peer Reviewed
• [Journal Article] Establishment of Induced Pluripotent Stem Cell Lines to Monitor the Silencing Status of Genomes (2013)
  • Author(s): Kenichiro Donai, Kengo Kuroda, Yijie Guo, Kyoung-Ha So, Hideko Sone, Masayuki Kobayashi, Katsuhiko Nishimori, Tomokazu Fukuda
  • Journal Title: Analytical Biochemistry Volume: 20
  • Peer Reviewed
• [Journal Article] Efficient establishment of pig embryonic fibroblast cell lines with conditional expression of simian vacuolating virus 40 large T fragment. (2012)
  • Author(s): Fukuda, T., Katayama, M., Yoshizawa, T., Eitsuka, T., Mizukami, H., Nakagawa, K., Ito, H., Komagata, H., Song, S., Roh, S., Sato, E., Hanada, H., Nishimori, K., Miyazawa, T., and Uchida, T
  • Journal Title: Bioscience Biotechnology and Biochemistry Volume: in press
  • NAID: 10030982228
  • Peer Reviewed
• [Journal Article] Antiproliferative effect of cathelicidin family peptide LL-37 analogue on colon cancer HCT116 p53+/+ and p53-/- cells. (2012)
  • Author(s): Kuroda, K., Fukuda, T., Yoneyama, H., Katayama, M., Isogai, H., Okumura, K., and Isogai, M
  • Journal Title: Oncology Rep Volume: in press
  • Peer Reviewed
• [Presentation] 人工多能性幹細胞におけるLTRプロモーターのサイレンシング状態を培養上清から簡便にモニタリングできるレポーターシステムの樹立 (2013)
  • Author(s): 土内憲一郎, 黒田健吾, 郭熠洁, 蘇敬夏, 小林正之, 西森克彦, 福田智一
  • Organizer: 日本繁殖生物学会総会
  • Place of Presentation: 東京農工大学
• [Presentation] 人工多能性幹細胞におけるLTRプロモーターのサイレンシング状態を培養上清から簡便にモニタリングできるレポーターシステムの樹立 (2013)
  • Author(s): 土内憲一郎、黒田健吾、小林正之、西森克彦、福田智一
  • Organizer: 106回日本繁殖生物学会
  • Place of Presentation: 東京農工大学
• [Presentation] Establishment of induced pluripotent stem cell lines that allow monitoring of the re-programming status of genomes (2012)
  • Author(s): Donai K., Kuroda K., So KH., Inagaki A., Nishimori K., Fukuda T
  • Organizer: Annual and International Meeting of the Japanese Association for Animal Cell Technology
  • Place of Presentation: Nagoya
• [Presentation] Application of induced pluripotent stem cells for the screening of low molecular demethylating compounds (2012)
  • Author(s): Fukuda T
  • Organizer: Annual and International Meeting of the Japanese Association for Animal Cell Technology
  • Place of Presentation: Nagoya
• [Presentation] Application of induced pluripotent stem cells for the screening of low-molecular demethylating compounds (2012)
  • Author(s): Tomokazu Fukuda
  • Organizer: International Symposium of Japanese Association for Animal Cell Technology
  • Place of Presentation: Nagoya
  • Invited