Study of the memory of genome stress

• Project/Area Number: 23650588
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Carcinogenesis
• Research Institution: Hiroshima University
• Principal Investigator: TASHIRO Satoshi 広島大学, 原爆放射線医科学研究所, 教授 (20243610)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 放射線 / 抗がん剤 / ゲノム損傷 / クロマチン / クロマチン免疫沈降シーケンス法 / クロマチン免疫沈降シーケンス報 / RAD51 / H2Aバリアント

Research Abstract

The purpose of this study is to test whether there is a system to memory the information of genome damage induced by ionizing radiation or anti-cancer drugs in human cells or not. We performed genome-wide analysis of the binding of DNA repair protein RAD51 to chromatin after induction of DNA damage. We could detect the binding of RAD51 to damaged sites and sub-telomeric regions at 2 hours after induction of DNA damage. The RAD51 binding to the damaged sites were reduced at two weeks after induction of DNA damage. We are now examining the binding of other repair factors and histones to the damaged sites at two weeks after induction of DNA damage.

Research Products

• [Journal Article] Involvement of ribonucleotide reductase-M1 in 5-fluorouracil-induced DNA damage in esophageal cancer cell lines. (2013)
  • Author(s): Aoki Y
  • Journal Title: Int J Oncol Volume: in press
• [Journal Article] A functional deficiency of TERA/VCP/p97 contributes to impaired DNA damage repair in multiple polyglutamine diseases. (2013)
  • Author(s): Fujita K
  • Journal Title: Nature commun Volume: in press
• [Journal Article] Shi L, Fujioka K, Sun J, Kinomura A, Inaba T, Ikura T, Ohtaki M, Yoshida M, Kodama Y, Livingston GK, Kamiya K, Tashiro S (2012)
  • Author(s): Shi L, Fujioka K, Sun J, Kinomura A, Inaba T, Ikura T, Ohtaki M, Yoshida M, Kodama Y, Livingston GK, Kamiya K, Tashiro S
  • Journal Title: Radiat Res Volume: 177(5) Pages: 533-538
  • Peer Reviewed
• [Journal Article] Investigation on circular asymmetry of geographical distribution in cancer mortality of Hiroshima atomic bomb survivors based on risk maps: analysis of spatial survival data (2012)
  • Author(s): Tonda T, Satoh K, Otani K, Sato Y, Maruyama H, Kawakami H, Tashiro S, Hoshi M, Ohtaki M
  • Journal Title: Radiat Environ Biophys Volume: 51(2) Pages: 133-141
  • Peer Reviewed
• [Journal Article] A modified system for analyzing ionizing radiation-induced chromosome abnormalities. (2012)
  • Author(s): Shi L
  • Journal Title: Radiat Res. Volume: -
  • Peer Reviewed
• [Journal Article] Synaptonemal complex protein SYCP3 impairs mitotic recombination by interfering with BRCA2 (2012)
  • Author(s): Hosoya N
  • Journal Title: EMBO Rep Volume: 13 Issue: 1 Pages: 44-51
  • DOI: 10.1038/embor.2011.221
  • Peer Reviewed
• [Journal Article] Synaptonemal complex protein SYCP3 impairs mitotic recombination by interfering with BRCA2 (2011)
  • Author(s): Hosoya N, Okajima M, Kinomura A, Fujii Y, Hiyama T, Sun J, Tashiro S, Miyagawa K
  • Journal Title: EMBO Rep Volume: 13(1) Pages: 44-51
  • Peer Reviewed
• [Journal Article] Halenaquinone, a chemical compound that specifically inhibits the secondary DNA binding of RAD51 (2011)
  • Author(s): Takaku M, Kainuma T, Ishida-Takaku T, Ishigami S, Suzuki H, Tashiro S, van Soest RW, Nakao Y, Kurumizaka H
  • Journal Title: Genes Cells Volume: 16(4) Pages: 427-436
  • Peer Reviewed
• [Journal Article] Halenaquinone, a chemical compound that specifically inhibits the secondary DNA binding of RAD51 (2011)
  • Author(s): Takaku M, Kainuma T, Ishida-Takaku T, Ishigami S, Suzuki H, Tashiro S, van Soest RWM, Nakao Y, Kurumizaka H
  • Journal Title: Genes Cells Volume: 6 Issue: 4 Pages: 427-436
  • DOI: 10.1111/j.1365-2443.2011.01494.x
  • Peer Reviewed
• [Journal Article] Involvement of ribonucleotide reductase-M1 in 5-fluorouracil-induced DNA damage in esophageal cancer cell lines
  • Author(s): Aoki Y, Sakogawa K, Hihara J, Emi M, Hmamai Y, Kono K, Shi L, Sun J, Kitao H, Ikura T, Niida H, Nakanishi M, Okada M, Tashiro S
  • Journal Title: Int J Oncol Volume: (in press)
  • Peer Reviewed
• [Journal Article] A functional deficiency of TERA/VCP/p97 contributes to impaired DNA damage repair in multiple polyglutamine diseases
  • Author(s): Fujita K, Nakamura Y, Oka T, Ito H, Tamura T, Tagawa K, Sasabe T, Katsuta A, Motoki K, Shiwaku H, Sone M, Yoshida C, Katsuno M, Sishi Y, Murata M, Taylor JP, Wanker EE, Kono K, Tashiro S, Sobue G, La Spada AR, Okazawa H
  • Journal Title: Nature commun Volume: (in press)
  • Peer Reviewed
• [Presentation] Mechanism of the radiation-induced repair focus formation (2012)
  • Author(s): Satoshi Tashiro, Jiying Sun, Kazuteru Kono, Hidekazu Suzuki, Tsuyoshi Ikura
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] Analysis of ionizing radiation-induced chromosome abnormalities by PNA-FISH (2012)
  • Author(s): Lin Shi, Kurumi Fujioka, Jiying Sun, Tsuyoshi Ikura, Megu Ohtaki, Mitsuaki Yoshida, Yoshiaki Kodama, Gordon K. Livingston, Kenji Kamiya, Satoshi Tashiro
  • Organizer: The 10thInternational Symposium on Chromosomal Aberrations (ISCA10)
  • Place of Presentation: Amalfi(イタリア)
  • Year and Date: 2012-10-19
• [Presentation] 放射線の健康影響と小児 (2012)
  • Author(s): 田代 聡
  • Organizer: 第115回日本小児科学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2012-04-20
• [Presentation] 放射線誘発核内フォーカス形成のエピゲノム制御 (2012)
  • Author(s): 田代 聡
  • Organizer: 日本農芸化学会2012 年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-22
• [Presentation] Analysis of ionizing radiation-induced chromosome abnormalities by PNA-FISH. (2012)
  • Author(s): Satoshi Tashiro
  • Organizer: The 10th International Symposium on Chromosomal Aberrations
  • Place of Presentation: Amalfi（イタリア）
• [Presentation] DNA 損傷応答におけるヒストンバリアント H2A.Z isoform の関与 (2011)
  • Author(s): 西淵いくの, 田代 聡, 原田昌彦, 深川竜郎, 井倉毅, 鈴木秀和, 木野村愛子, 孫継英
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] A new system for analyzing ionizing radiation-induced chromosome abnormalities (2011)
  • Author(s): 時林,藤岡来実,孫継英,木野村愛子,稲葉俊哉,井倉毅,大瀧慈,吉田光明,児玉吉明, Gordon K. Livingston, 神谷研二,田代聡
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] 5-Fluorouracil誘導性 DNA 障害におけるribonucleotide reductase-1 の役割に関する検討 (2011)
  • Author(s): 青木義朗,田代 聡
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: v
  • Year and Date: 2011-12-13
• [Presentation] 放射線被ばくによるゲノム損傷と小児の健康影響 (2011)
  • Author(s): 田代 聡
  • Organizer: 第53回日本小児血液・がん学会学術集会
  • Place of Presentation: 前橋
  • Year and Date: 2011-11-25
• [Presentation] DNA 損傷応答におけるヒストンバリアント H2A.Z isoform の関与 (2011)
  • Author(s): 西淵いくの,田代聡,原田昌彦,木村智樹,村上祐司,権丈雅浩,兼安祐子,永田靖
  • Organizer: 日本放射線腫瘍学会第24回大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-17
• [Presentation] A new system for analyzing ionizing radiation-induced chromosome abnormalities (2011)
  • Author(s): 時林,藤岡来実,孫継英,木野村愛子,稲葉俊哉,井倉毅,大瀧慈,吉田光明,児玉吉明, Gordon K. Livingston,神谷研二,田代聡
  • Organizer: 日本放射線影響学会 第54回大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-17
• [Presentation] ATM modulates the loading of recombination proteins onto a chromosomal translocation breakpoint hotspot (2011)
  • Author(s): Jiying Sun, Yukako Oma, Masahiko Harata, Kazuteru Kono, Hiroki Shima, Aiko Kinomura, Tsuyoshi Ikura, Hidekazu Suzuki, Shuki Mizutani, Roland Kanaar and Satoshi Tashiro
  • Organizer: 第54回放射線影響学会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-17
• [Presentation] 放射線により誘発される DNA修復蛋白質による核内高次構造体形成、福島原子力発電所事故における現地での活動報告 (2011)
  • Author(s): 田代 聡
  • Organizer: 日本薬物動態学会 第26回年会
  • Place of Presentation: 広島
  • Year and Date: 2011-11-17
• [Presentation] The dynamics of histone variant H2A.Z upon DNA damage (2011)
  • Author(s): Ikuno Nishibuchi, Satoshi Tashiro,Hiroki Shima, Tomoki Kimura, Yuji Murakami, Masahiro Kenjo, Yuko Kaneyasu, Yasushi Nagata
  • Organizer: ASTRO's 53rdAnnual Meeting
  • Place of Presentation: Miami, USA
  • Year and Date: 2011-11-02
• [Presentation] DNA 二重鎖切断に着目した 5-Fluorouracil の作用機序の解明 (2011)
  • Author(s): 青木義朗,檜原 淳,迫川賢士,恵美 学,浜井洋一,岡田守人, 田代 聡
  • Organizer: 第65回食道学会学術集会
  • Place of Presentation: 仙台
  • Year and Date: 2011-09-26
• [Presentation] A new system for analyzing ionizing radiation-induced chromosome abnormalities (2011)
  • Author(s): 時林,藤岡来実,孫継英,木野村愛子,稲葉俊哉,井倉毅,大瀧慈,吉田光明,児玉吉明, Gordon K. Livingston, 神谷研二, 田代 聡
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-21
• [Presentation] Mechanism of 11q23 chromosomal translocation after etoposide treatment (2011)
  • Author(s): Jiying Sun, Aiko Kinomura, Hidekazu Suzuki, Tsuyoshi Ikura, Dipanjan Chowdhury and Satoshi Tashiro
  • Organizer: 第84回生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-21
• [Presentation] 放射線発がん分子メカニズム研究の新展開 (2011)
  • Author(s): 田代 聡
  • Organizer: 第28回日本医学会総会特別企画
  • Place of Presentation: 京都
  • Year and Date: 2011-09-17
• [Presentation] 原発事故と小児放射線健康影響 (2011)
  • Author(s): 田代 聡
  • Organizer: 第114回日本小児科学会
  • Place of Presentation: 東京
  • Year and Date: 2011-08-12
• [Presentation] DNA二重鎖切断に着目した 5-Fluorouracilの作用機序の解明 (2011)
  • Author(s): 青木義朗,迫川賢士,檜原淳,恵美学,浜井洋一,岡田守人,田代聡
  • Organizer: 第66回日本消化器外科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-07-13
• [Presentation] 福島県における小児甲状腺被ばく調査 (2011)
  • Author(s): 名古屋
  • Organizer: 日本小児科学会緊急フォーラム
  • Place of Presentation: 東京
  • Year and Date: 2011-04-17
• [Presentation] 原発事故と小児放射線健康影響（シンポジウム） (2011)
  • Author(s): 田代 聡
  • Organizer: 第114回日本小児科学会(招待講演)
  • Place of Presentation: 東京都
• [Presentation] A new system for analyzing the ionizing irradiation-induced chromosome abnormalities. (2011)
  • Author(s): Shi L
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: ポーランド
• [Presentation] 放射線発がん分子メカニズム研究の新展開（シンポジウム） (2011)
  • Author(s): 田代 聡
  • Organizer: 第28回日本医学会総会特別企画(招待講演)
  • Place of Presentation: 東京都
• [Presentation] Mechanism of 11q23 chromosomal translocation after etoposide treatment (2011)
  • Author(s): 孫 継英
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都市
• [Presentation] A new system for analyzing the ionizing irradiation-induced chromosome abnormalities. (2011)
  • Author(s): 時 林
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都市
• [Presentation] 放射線による染色体異常の解析のための新しい方法 (2011)
  • Author(s): 時 林
  • Organizer: 日本放射線影響学会 第54回大会
  • Place of Presentation: 神戸市
• [Presentation] 放射線被ばくによるゲノム損傷と小児の健康影響（イブニングセミナ） (2011)
  • Author(s): 田代 聡
  • Organizer: 第53回日本小児血液・がん学会学術集会(招待講演)
  • Place of Presentation: 高崎市
• [Presentation] DNA損傷応答におけるヒストンバリアントH2A.Z isoformの関与 (2011)
  • Author(s): 西淵 いくの
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市
• [Presentation] 放射線誘発がんと染色体異常
  • Author(s): 田代 聡
  • Organizer: 第35回日本がん疫学・分子疫学研究会総会(招待講演)
  • Place of Presentation: 広島
• [Presentation] 細胞核高次構造に基づいた生物学的線量評価指標の開発
  • Author(s): 田代 聡
  • Organizer: 日本放射線影響学会第55回大会
  • Place of Presentation: 仙台
• [Presentation] Detection of radiation-induced chromosome abnormalities using FISH with PNA probes
  • Author(s): Satoshi Tashiro
  • Organizer: 第71回日本癌学会総会
  • Place of Presentation: 札幌
• [Presentation] Mechanism of the radiation-induced repair focus formation.
  • Author(s): Satoshi Tashiro
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/cellbio/cell_biology/Welcome_%28youkoso%29.html