| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Our knowledge of the factors that induce aberrant DNA methylation is limited. The present study aimed to develop an assay system to detect aberrant DNA methylation in vivo. The vector, in which LacI was inserted into downstream of the promoter CpG island, and the vector, in which EGFP as the reporter gene was inserted into downstream of lacO, were stably introduced into a human colon cancer cell line. The cells induced aberrant methylation of the promoter with green fluorescence during xenograft tumor formation in nude mice. In the tumor without fluorescence, no methylation was induced. These results indicate that cell lines constructed in this study may have the ability to detect induction of aberrant methylation. In the next step, construction of transgenic mouse using this strategy would be expected.
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