Development of cancer stem cell (CSC) culturing system and identification of CSC-specific marker
| Project/Area Number |
23650597
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Tottori University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YUGE Rui 広島大学, 保健学研究科, 教授 (20263676)
KAWAHARA Yumi 広島大学, 保健学研究科, 研究員 (20457279)
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| Research Collaborator |
ITO Hisao 鳥取大学, 理事 (60127610)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 幹細胞 / がん幹細胞 / 微小重力 |
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| Research Abstract |
We cultured cancer cells with high drug-resistant ability, using a three-dimentional (3D) clinostat, a device which produces an environment with an average of 10-3G over the time, as a culture method possibly capable of maintaining stemness of cancer stem cells. By culturing in the microgravity environment, cell growth was promoted compared to that in the normal gravity environment. We inoculated 1ｘ102～103cells cultured in the environment subcutaneously in nude mice, and found no tumorigenicity maintained, indicating that the culture under the microgravity environment was not sufficient to maintain highly purified cancer stem cells.
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Report
(3 results)
Research Products
(5 results)
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[Journal Article] MicroRNA-143 Regulates Human Osteosarcoma Metastasis by Regulating Matrix Metalloprotease-13 Expression2011
Author(s)
Osaki M, Takeshita F, Sugimoto Y, Kosaka N, Yamamoto Y, Yoshioka Y, Kobayashi E, Yamada T, Kawai A, Inoue T, Ito H, Oshimura M, Ochiya T
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Journal Title
Molecular Therapy
Volume: 19(6)
Issue: 6
Pages: 1123-1130
DOI
Related Report
Peer Reviewed
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