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Development of cancer stem cell (CSC) culturing system and identification of CSC-specific marker

Research Project

Project/Area Number 23650597
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Tumor biology
Research InstitutionTottori University

Principal Investigator

OSAKI Mitsuhiko  鳥取大学, 医学部, 准教授 (40325006)

Co-Investigator(Renkei-kenkyūsha) YUGE Rui  広島大学, 保健学研究科, 教授 (20263676)
KAWAHARA Yumi  広島大学, 保健学研究科, 研究員 (20457279)
Research Collaborator ITO Hisao  鳥取大学, 理事 (60127610)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords幹細胞 / がん幹細胞 / 微小重力
Research Abstract

We cultured cancer cells with high drug-resistant ability, using a three-dimentional (3D) clinostat, a device which produces an environment with an average of 10-3G over the time, as a culture method possibly capable of maintaining stemness of cancer stem cells. By culturing in the microgravity environment, cell growth was promoted compared to that in the normal gravity environment. We inoculated 1x102~103cells cultured in the environment subcutaneously in nude mice, and found no tumorigenicity maintained, indicating that the culture under the microgravity environment was not sufficient to maintain highly purified cancer stem cells.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (5 results)

All 2012 2011

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] マイクロRNAによるがん転移予防への展開2012

    • Author(s)
      尾崎充彦
    • Journal Title

      臨床・創薬が見えてきたmicroRNA

      Volume: 23 Pages: 151-156

    • Related Report
      2012 Final Research Report
  • [Journal Article] マイクロRNAによるがん転移予防への展開2012

    • Author(s)
      尾崎充彦
    • Journal Title

      臨床・創薬利用が見えてきたmicroRNA

      Volume: 23 Pages: 151-156

    • Related Report
      2012 Annual Research Report
  • [Journal Article] MicroRNA-143 Regulates Human Osteosarcoma Metastasis by Regulating Matrix Metalloprotease-13 Expression2011

    • Author(s)
      Osaki M, Takeshita F, Sugimoto Y, Kosaka N, Yamamoto Y, Yoshioka Y, Kobayashi E, Yamada T, Kawai A, Inoue T, Ito H, Oshimura M, Ochiya T
    • Journal Title

      Molecular Therapy

      Volume: 19(6) Issue: 6 Pages: 1123-1130

    • DOI

      10.1038/mt.2011.53

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒト骨肉腫肺転移抑制効果を示すmiR-143の標的遺伝子の同定2011

    • Author(s)
      尾崎充彦、杉本結衣、知念日菓利、竹下文隆、落谷孝広、井藤久雄、押村光雄
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2011-10-05
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト骨肉腫肺転移抑制効果を示すmiR-143の標的遺伝子の同定2011

    • Author(s)
      尾崎充彦、杉本結衣、知念日菓利、竹下文隆、落谷孝広、井藤久雄、押村光雄
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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