| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
In order to visualize Epithelial to Mesenchymal Transition (EMT) in vivo, I tried to develop a fluorescent probe for EMT by combination ofEMT-specific promoters and a Bimolecular Fluorescence Complementation (BiFC) method. I showed that a BiFC method worked in vivo using LZA and LZB in cells. Moreover, I established an in vivo imaging system for mKO, mCherry, CFP and Venus in living mice. Finally, I screened EMT related promoters and showed that promoters for twist and snail are good candidate for the system.
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