Development of anti-metastatic strategy based on analysis of premetastatic niche

• Project/Area Number: 23650601
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Keio University
• Principal Investigator: SAYA Hideyuki 慶應義塾大学, 医学部, 教授 (80264282)
• Project Period (FY): 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 癌 / 遺伝子 / 細胞・組織 / 病理学 / シグナル伝達

Research Abstract

The step which cancer cells establish their metastatic lesions in other remote organs is dependent on the survival activity of cancer cells and the microenvironment of the secondary lesion. This project was conducted to clarify the molecular mechanism of the interaction between cancer cells and the premetastatic niche. We injected human breast cancer cells into mouse heart and obtained the brain metastasis lesions followed by the RNA-seq analysis of mRNA derived from both mouse cells and human cells. This experiments allowed us to identify the specific gene sets which may play a role in colonization of cancer cells in the secondary lesion.

Research Products

• [Journal Article] Induction of ZEB Proteins by Inactivation of RB Protein Is Key Determinant of Mesenchymal Phenotype of Breast Cancer (2012)
  • Author(s): Yoshimi Arima, Hidemi Hayashi, Mikako Sasaki, Mari Hosonaga, Takaaki M. Goto, Tatsuyuki Chiyoda, Shinji Kuninaka, Tatsuhiro Shibata, Hirokazu Ohata, Hitoshi Nakagama, Yoichi Taya, Hideyuki Saya
  • Journal Title: J Biol Chem Volume: 287 Pages: 7896-7906
• [Journal Article] Induction of ZEB by inactivation of RB is a key determinant of the mesenchymal phenotype of breast cancer (2012)
  • Author(s): Arima Y, Saya H, et al
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 11 Pages: 7896-7906
  • DOI: 10.1074/jbc.m111.313759
  • Peer Reviewed
• [Journal Article] Loss of p16 expression is associated with the stem cell characteristics of surface markers and therapeutic resistance in estrogen receptor-negative breast cancer (2012)
  • Author(s): Arima Y, Saya H, et al
  • Journal Title: InternationalJournal of Cancer Volume: 130 Issue: 11 Pages: 2568-2579
  • DOI: 10.1002/ijc.26271
  • Peer Reviewed
• [Journal Article] Loss of p16 expression is associated with the stem cell characteristics of sureface markers and therapeutic resistance in estrogen receptor-negative breast cancer (2011)
  • Author(s): Arima Y, Naoki Hayashi, Hidemi Hayashi, Mikako Sasaki, Kazuharu Kai, Eiji Sugihara, Eriko Abe, Atsushi Yoshida, Shuji Mikami, Seigo Nakamura, Hideyuki Saya
  • Journal Title: Int J Cancer Volume: 130 Pages: 2568-2579
• [Presentation] Role of CD44 in cancer stem cells and metastasis. Prenary Lecture5 (2011)
  • Author(s): Saya H
  • Organizer: 8^<th> International Symposium on Residual Cancer
  • Place of Presentation: Osaka
  • Year and Date: 2011-09-23
• [Presentation] Role of CD44 in cancer stem cells and metastasis (2011)
  • Author(s): Saya H
  • Organizer: Plenary Lecture 5, 8^<th> International Symposium on Minmal Residual Cancer
  • Place of Presentation: Osaka International Convention Center, Osaka(招待講演)
  • Year and Date: 2011-09-23
• [Remarks]
  • URL: http://www.genereg.jp