Specific recovery of exhausted T cells against HTLV-1

• Project/Area Number: 23650606
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor immunology
• Research Institution: Hokkaido University
• Principal Investigator: SHIDA Hisatoshi 北海道大学, 遺伝子病制御研究所, 教授 (00144395)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: HTLV-1 / 消耗T細胞 / 免疫療法 / ターゲッティング / CTL

Research Abstract

We constructed the molecule that specifically recognizes antiHTLV-1 Tax CTL. The cells that express both this molecule and measles virus F proteinare able to fuse with Tax CTL. The vector that possesses both this molecule and F proteinon its surface may be expected to specifically introduce the genes into Tax CTL.

Research Products

• [Journal Article] Elicitation of both anti HIV-1 Env humoral and cellular immunities by replicating Vaccinia prime Sendai virus boost regimen and boosting by CD40Lm. (2012)
  • Author(s): Xianfeng Zhang, Tomoyoshi Sobue, Mao Isshiki, Shun-ichi Makino, Makoto Inoue, Kazunori Kato, Tatsuo Shioda, Takashi Ohashi, Jun Komano, Hideji Hanabusa Hisatoshi Shida
  • Journal Title: Plos One Volume: 7
  • Peer Reviewed
• [Journal Article] Role of nucleocytoplasmic RNA transport during thelife cycle of retroviruses. (2012)
  • Author(s): Hisatoshi Shida
  • Journal Title: Frontiers in Microbiology. Volume: 3
  • Peer Reviewed
• [Journal Article] Flow cytometry based identification of simian immunodeficiency virus Env-specific B lymphocytes. (2011)
  • Author(s): Fofana IB, Colantonio AD, Reeves RK, Connole MA, Gillis JM, Hall LR, Sato S, Audin CR, Evans DT, Shida H, Johnson RP, Johnson WE
  • Journal Title: J Immunol Methods. Volume: 370 Pages: 75-85
  • Peer Reviewed
• [Journal Article] MicroRNA Regulation of Glycoprotein B5R in Oncolytic Vaccinia Virus (2011)
  • Author(s): Mina Hikichi, Minoru Kidokoro, Takeshi Haraguchi, Hideo Iba, Hisatoshi Shida, Hideaki Tahara, Takafumi Nakamura
  • Journal Title: Reduces Viral Pathogenicity without Impairing its Antitumor Efficacy. Mol. Ther. Volume: 19 Pages: 1107-15
  • Peer Reviewed
• [Journal Article] Nuclear and Cytoplasmic Effects of Human CRM1 on HIV-1 (2011)
  • Author(s): Mika Nagai-Fukataki, Takashi Ohashi, Iwao Hashimoto, Tominori Kimura, Yoshiyuki Hakata, Hisatoshi Shida
  • Journal Title: Production in Rat Cells. Genes to Cells Volume: 16 Pages: 203-216
  • Peer Reviewed
• [Journal Article] Nuclear and Cytoplasmic Effects of Human CRM1 on HIV-1 Production in Rat Cells. Genes to Cells (2011)
  • Author(s): Mika Nagai-Fukataki, Takashi Ohashi, Iwao Hashimoto, Tominori Kimura, Yoshiyuki Hakata, Hisatoshi Shida
  • Journal Title: Genes to Cells Volume: 16 Pages: 203-216
• [Presentation] 弱毒ワクシニアプライム、センダイウイルスブーストエイズワクチン法の免疫評価およびhCD40Lmアジュバント効果の検討。 (2012)
  • Author(s): 張険峰
  • Organizer: 日本エイズ学会
  • Place of Presentation: 横浜慶応義塾大学日吉キャンパス
  • Year and Date: 2012-11-24
• [Presentation] Protection against highly pathogenic SIV by BCG-SIV recombinant priming and attenuated replicating vaccinia-SIV recombinant boosting (2012)
  • Author(s): Xianfeng Zhang, Hiromi Sakawaki, Tomoyuki Miura, Tatsuhiko Igarashi, Shigeo Horibata, Kaori Yokomizo, Kazuhiro Matsuo, Naoki Yamamoto, Ismael Ben Fofana, Welkin Johnson, Takashi Ohashi, Hisatoshi Shida
  • Organizer: AIDS VCCINE2012.
  • Place of Presentation: Convention Center,Boston, USA
• [Presentation] Enhancement of CD40Lm on Vaccine Elicited Anti-HIV-1 Immunity (2011)
  • Author(s): Xianfeng Zhang, Tomoyoshi Sobue, Hisatoshi Shida
  • Organizer: 6th IAS Conference on HIV Pathogenesis, Treatment and Prevention.
  • Place of Presentation: Rome, Italy.
• [Presentation] Immunogenicity of Lc16M8〓 Vaccinia Prime/Sendai Virus Vector Boost Targeting the Envelope Glycoprotein of HIV-1 and Contribution of CD40Lm (2011)
  • Author(s): Tomoyoshi Sobue, Shun-Ichi Makino, Xianfeng Zhang, Takashi Ohashi, Kazunori Kato, Tatsuo Shioda, Makoto Inoue, Mamoru Hasegawa, Hisatoshi Shida
  • Organizer: XV International Congress of Virology.
  • Place of Presentation: Sapporo Convention Center, Sapporo, Japan.
• [Presentation] Immunogenicity of LC16m8〓 Vaccinia Virus/Sendai Virus Vector Expressing the gp160 of HIV-1 and Effect of CD40Lm (2011)
  • Author(s): H. Shida
  • Organizer: AIDS VCCINE 2011
  • Place of Presentation: Intercontinental hotel, Bangkok Thailand
• [Presentation] ラットT細胞へのHIV-1感染におけるCSA,As203の増強効果
  • Author(s): 宮崎かや、張険峰、大橋貴、志田壽利
  • Organizer: 第２５回日本エイズ第学会学術総会
  • Place of Presentation: ハイアットリージェンシー東京 東京
• [Remarks]
  • URL: http://www.igm.hokudai.ac.jp/molvir
• [Patent(Industrial Property Rights)] ワクシニアウイルスベクターおよびセンダイウイルスベクターからなるプライム/ブーストワクチン用ウイルスベクター (2013)
  • Inventor(s): 志田壽利、祖父江友芳、加藤和則、井上誠、長谷川護
  • Industrial Property Rights Holder: 国立大学法人北海道大学、北海道公立大学法人札幌医科大学、ディナベック株式会社
  • Filing Date: 2013-04-17
  • Overseas